Arginyl tRNA synthetase (RARS) (NM_002887) Human Tagged ORF Clone

CAT#: RG201261

  • TrueORF®

RARS (tGFP-tagged) - Human arginyl-tRNA synthetase (RARS)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_002887" in other vectors (6)

Reconstitution Protocol

USD 814.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Other products for "Arginyl tRNA synthetase"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol Arginyl tRNA synthetase
Synonyms ArgRS; DALRD1; HLD9; RARS
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG201261 representing NM_002887
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTACTGGTGTCTGAGTGCTCCGCGCGGCTGCTGCAGCAGGAAGAAGAGATTAAATCTCTGACTG
CTGAAATTGACCGGTTGAAAAACTGTGGCTGTTTAGGAGCTTCTCCAAATTTGGAGCAGTTACAAGAAGA
AAATTTAAAATTAAAGTATCGACTGAATATTCTTCGAAAGAGTCTTCAGGCAGAAAGGAACAAACCAACT
AAAAATATGATTAACATTATTAGCCGCCTACAAGAGGTCTTTGGTCATGCAATTAAGGCTGCATATCCAG
ATTTGGAAAATCCTCCTCTGCTAGTGACACCAAGTCAGCAGGCCAAGTTTGGGGACTATCAGTGTAATAG
TGCTATGGGTATTTCTCAGATGCTCAAAACCAAGGAACAGAAAGTTAATCCAAGAGAAATTGCTGAAAAC
ATTACCAAACACCTCCCAGACAATGAATGTATTGAAAAAGTTGAAATTGCTGGTCCTGGTTTTATTAATG
TCCACTTAAGAAAGGATTTTGTATCAGAACAATTGACCAGTCTTCTAGTGAATGGAGTTCAACTACCTGC
TCTGGGAGAGAATAAAAAGGTTATAGTTGACTTTTCCTCCCCTAATATAGCTAAAGAGATGCATGTAGGC
CACCTGAGGTCAACTATCATAGGAGAGAGTATAAGCCGCCTCTTTGAATTTGCAGGGTATGACGTGCTCA
GGTTAAATCATGTAGGAGACTGGGGGACCCAGTTTGGCATGCTCATCGCTCACCTGCAAGACAAATTTCC
AGATTATCTAACAGTTTCACCTCCTATTGGGGATCTTCAGGTCTTTTATAAGGAATCTAAGAAGAGGTTT
GATACTGAGGAGGAATTTAAGAAGCGAGCATATCAGTGTGTAGTTCTGCTCCAGGGTAAAAACCCAGATA
TTACAAAAGCTTGGAAGCTTATCTGTGATGTCTCCCGCCAAGAGTTAAATAAAATCTATGATGCATTGGA
CGTCTCTTTAATAGAGAGAGGGGAATCCTTCTATCAAGATAGGATGAATGATATTGTAAAGGAATTTGAA
GATAGAGGATTTGTGCAGGTGGATGATGGCAGAAAGATTGTATTTGTCCCAGGGTGTTCCATACCATTAA
CCATAGTAAAATCAGATGGAGGTTATACCTATGATACATCTGACCTGGCTGCTATTAAACAAAGACTATT
TGAGGAAAAAGCAGATATGATTATCTATGTTGTGGACAATGGACAATCTGTGCACTTCCAGACAATATTT
GCTGCTGCTCAAATGATTGGTTGGTATGACCCTAAAGTAACTCGAGTCTTCCATGCTGGATTTGGTGTGG
TGCTAGGGGAAGACAAGAAAAAGTTTAAAACACGTTCGGGTGAAACAGTGCGCCTCATGGATCTTCTGGG
AGAAGGACTAAAACGATCCATGGACAAGTTGAAGGAAAAAGAAAGAGACAAGGTCTTAACTGCAGAGGAA
TTGAATGCTGCTCAGACATCCGTTGCATATGGCTGCATCAAATATGCTGACCTTTCCCATAACCGGTTGA
ATGACTACATCTTCTCCTTTGACAAAATGCTAGATGACAGAGGAAATACAGCTGCTTACTTGTTGTATGC
CTTCACTAGAATCAGGTCTATTGCACGTCTGGCCAATATTGATGAAGAAATGCTCCAAAAAGCTGCTCGA
GAAACCAAGATTCTTTTGGATCATGAGAAGGAATGGAAACTAGGCCGGTGCATTTTACGGTTCCCTGAGA
TTCTGCAAAAGATTTTAGATGACTTATTTCTCCACACTCTCTGTGATTATATATATGAGCTGGCAACTGC
TTTCACAGAGTTCTATGATAGCTGCTACTGTGTGGAGAAAGATAGACAGACTGGAAAAATATTGAAGGTG
AACATGTGGCGTATGCTGCTATGTGAAGCAGTAGCTGCTGTCATGGCCAAGGGGTTTGATATCCTGGGAA
TAAAACCTGTCCAAAGGATG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG201261 representing NM_002887
Red=Cloning site Green=Tags(s)

MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQLQEENLKLKYRLNILRKSLQAERNKPT
KNMINIISRLQEVFGHAIKAAYPDLENPPLLVTPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAEN
ITKHLPDNECIEKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPALGENKKVIVDFSSPNIAKEMHVG
HLRSTIIGESISRLFEFAGYDVLRLNHVGDWGTQFGMLIAHLQDKFPDYLTVSPPIGDLQVFYKESKKRF
DTEEEFKKRAYQCVVLLQGKNPDITKAWKLICDVSRQELNKIYDALDVSLIERGESFYQDRMNDIVKEFE
DRGFVQVDDGRKIVFVPGCSIPLTIVKSDGGYTYDTSDLAAIKQRLFEEKADMIIYVVDNGQSVHFQTIF
AAAQMIGWYDPKVTRVFHAGFGVVLGEDKKKFKTRSGETVRLMDLLGEGLKRSMDKLKEKERDKVLTAEE
LNAAQTSVAYGCIKYADLSHNRLNDYIFSFDKMLDDRGNTAAYLLYAFTRIRSIARLANIDEEMLQKAAR
ETKILLDHEKEWKLGRCILRFPEILQKILDDLFLHTLCDYIYELATAFTEFYDSCYCVEKDRQTGKILKV
NMWRMLLCEAVAAVMAKGFDILGIKPVQRM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002887
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002887.2
RefSeq Size 2154 bp
RefSeq ORF 1983 bp
Locus ID 5917
UniProt ID P54136
Cytogenetics 5q34
Domains tRNA-synt_1d, N-Arg
Protein Families Druggable Genome
Protein Pathways Aminoacyl-tRNA biosynthesis
Gene Summary Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008]

Other Versions

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