AP2B1 (NM_001030006) Human Tagged ORF Clone

CAT#: RG201129

AP2B1 (tGFP-tagged) - Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1

Product Images

Circular map for RG201129

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AP2B1
• Synonyms: ADTB2; AP2-BETA; AP105B; CLAPB1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201129 representing NM_001030006
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACTCCAAGTATTTCACAACCAATAAAAAAGGAGAAATATTTGAACTAAAAGCTGAACTCAACA
  ATGAAAAGAAAGAAAAGAGAAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGATGT
  TAGTTCTCTCTTTCCAGACGTAGTGAACTGTATGCAGACTGACAATCTGGAACTAAAGAAGCTTGTGTAT
  CTCTACTTGATGAACTACGCCAAGAGTCAGCCAGACATGGCCATCATGGCTGTAAACAGCTTTGTGAAGG
  ACTGTGAAGATCCTAATCCTTTGATTCGAGCCTTGGCAGTCAGAACCATGGGGTGCATCCGGGTAGACAA
  AATTACAGAATATCTCTGTGAGCCGCTCCGCAAGTGCTTGAAGGATGAGGATCCCTATGTTCGGAAAACA
  GCAGCAGTCTGCGTGGCAAAACTCCATGATATCAATGCCCAAATGGTGGAAGATCAGGGATTTCTGGATT
  CTCTACGGGATCTCATAGCAGATTCAAATCCAATGGTGGTGGCTAATGCCGTAGCGGCATTATCTGAAAT
  CAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCACAGAACATTAATAAGCTGCTGACAGCC
  CTGAATGAATGCACTGAATGGGGCCAGATTTTCATCCTGGACTGCCTGTCTAATTACAACCCTAAAGATG
  ATCGGGAGGCTCAGAGCATCTGTGAGCGGGTAACTCCCCGGCTATCCCATGCCAACTCAGCAGTGGTGCT
  TTCAGCGGTAAAAGTCCTAATGAAGTTTCTAGAATTGTTACCTAAGGATTCTGACTACTACAATATGCTG
  CTGAAGAAGTTAGCCCCTCCACTTGTCACTTTGCTGTCTGGGGAGCCAGAAGTGCAGTATGTCGCCCTGA
  GGAACATCAACTTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAAATCAAAGTCTTCTTTGTGAA
  GTACAATGATCCCATCTATGTTAAACTAGAGAAGTTGGACATCATGATTCGTTTGGCATCTCAAGCCAAC
  ATTGCTCAGGTTCTGGCAGAACTGAAAGAATATGCTACAGAGGTGGATGTTGACTTTGTTCGAAAAGCTG
  TGCGGGCCATTGGACGGTGTGCCATCAAGGTGGAGCAATCTGCAGAGCGCTGTGTAAGCACATTGCTTGA
  TCTAATCCAGACCAAAGTGAATTATGTGGTCCAAGAAGCAATTGTTGTCATCAGGGACATCTTCCGCAAA
  TACCCCAACAAGTATGAAAGTATCATCGCCACTCTGTGTGAGAACTTAGACTCGCTGGATGAGCCAGATG
  CTCGAGCAGCTATGATTTGGATTGTGGGAGAATATGCTGAAAGAATTGACAATGCAGATGAGTTACTAGA
  AAGCTTCCTGGAGGGTTTTCACGATGAAAGCACCCAGGTGCAGCTCACTCTGCTTACTGCCATAGTGAAG
  CTGTTTCTCAAGAAACCATCAGAAACACAGGAGCTAGTCCAGCAGGTCTTGAGTTTGGCAACACAGGATT
  CTGATAATCCTGACCTTCGAGACCGGGGCTATATTTATTGGCGCCTTCTCTCAACTGACCCTGTTACAGC
  TAAAGAAGTAGTCTTGTCTGAGAAGCCACTGATCTCTGAGGAGACGGACCTTATTGAGCCAACTCTGCTG
  GATGAGCTAATCTGCCACATTGGTTCTTTGGCCTCTGTGTATCATAAGCCTCCCAATGCTTTTGTGGAAG
  GAAGTCATGGAATTCATCGTAAACACTTGCCAATTCATCATGGGAGCACTGATGCAGGTGACAGCCCTGT
  TGGCACTACCACTGCAACGAACCTGGAACAGCCTCAGGTTATCCCCTCTCAAGGTGATCTTCTAGGGGAT
  CTTTTAAACCTTGACCTCGGTCCCCCAGTCAATGTGCCACAGGTGTCCTCCATGCAGATGGGAGCAGTGG
  ATCTCCTAGGAGGAGGACTAGATAGTCTGCTTGGCAGTGACCTTGGCGGGGGCATTGGAGGAAGTCCGGC
  AGTGGGACAATCCTTCATCCCATCATCGGTCCCTGCAACCTTTGCTCCTTCACCTACACCTGCTGTGGTC
  AGCAGTGGACTGAATGACCTGTTTGAACTCTCCACAGGGATAGGCATGGCACCTGGTGGATATGTGGCTC
  CTAAGGCTGTCTGGCTACCTGCAGTAAAGGCTAAAGGCTTGGAGATTTCCGGAACATTTACTCACCGCCA
  AGGGCACATCTATATGGAAATGAACTTCACCAATAAAGCTCTGCAGCACATGACAGATTTTGCAATCCAG
  TTTAACAAAAATAGCTTTGGTGTCATCCCCAGCACTCCTCTGGCCATCCATACACCACTGATGCCAAACC
  AGAGCATTGATGTCTCCCTGCCTCTCAATACCTTGGGCCCAGTCATGAAGATGGAACCTCTGAATAACCT
  CCAGGTGGCTGTGAAAAACAATATCGATGTCTTCTACTTCAGCTGCCTCATCCCACTCAATGTGCTTTTT
  GTAGAAGATGGCAAAATGGAGCGCCAGGTCTTCCTTGCAACATGGAAGGATATTCCCAATGAAAATGAAC
  TTCAGTTTCAGATTAAGGAATGTCATTTAAATGCTGACACTGTTTCCAGCAAGTTGCAAAACAACAATGT
  TTATACTATTGCCAAGAGGAATGTGGAAGGGCAGGACATGCTGTACCAATCCCTGAAGCTCACTAATGGC
  ATTTGGATTTTGGCCGAACTACGTATCCAGCCAGGAAACCCCAATTACACGCTGTCACTGAAGTGTAGAG
  CTCCTGAAGTCTCTCAATACATCTATCAGGTCTACGACAGCATTTTGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201129 representing NM_001030006
  Red=Cloning site Green=Tags(s)
  
  MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVY
  LYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT
  AAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTA
  LNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNML
  LKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQAN
  IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRK
  YPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVK
  LFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLL
  DELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTATNLEQPQVIPSQGDLLGD
  LLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLLGSDLGGGIGGSPAVGQSFIPSSVPATFAPSPTPAVV
  SSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQ
  FNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLF
  VEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNG
  IWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001030006
• ORF Size: 2853 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030006.1, NP_001025177.1
• RefSeq Size: 5772 bp
• RefSeq ORF: 2856 bp
• Locus ID: 163
• UniProt ID: P63010
• Cytogenetics: 17q12
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]