CIKS (TRAF3IP2) (NM_147686) Human Tagged ORF Clone

CAT#: RG201106

TRAF3IP2 (tGFP-tagged) - Human TRAF3 interacting protein 2 (TRAF3IP2), transcript variant 2

Product Images

Circular map for RG201106

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CIKS
• Synonyms: ACT1; C6orf2; C6orf4; C6orf5; C6orf6; CANDF8; CIKS; PSORS13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201106 representing NM_147686
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGAAGCATTCCTGTGGAGGTTGATGAATCAGAACCATACCCAAGTCAGTTGCTGAAACCAATCC
  CAGAATATTCCCCGGAAGAGGAATCAGAACCACCTGCTCCAAATATAAGGAACATGGCACCCAACAGCTT
  GTCTGCACCCACAATGCTTCACAATTCCTCCGGAGACTTTTCTCAAGCTCACTCAACCCTGAAACTTGCA
  AATCACCAGCGGCCTGTATCCCGGCAGGTCACCTGCCTGCGCACTCAAGTTCTGGAGGACAGTGAAGACA
  GTTTCTGCAGGAGACACCCAGGCCTGGGCAAAGCTTTCCCTTCTGGGTGCTCTGCAGTCAGCGAGCCTGC
  GTCTGAGTCTGTGGTTGGAGCCCTCCCTGCAGAGCATCAGTTTTCATTTATGGAAAAACGTAATCAATGG
  CTGGTATCTCAGCTTTCAGCGGCTTCTCCTGACACTGGCCATGACTCAGACAAATCAGACCAAAGTTTAC
  CTAATGCCTCAGCAGACTCCTTGGGCGGTAGCCAGGAGATGGTGCAACGGCCCCAGCCTCACAGGAACCG
  AGCAGGCCTGGATCTGCCAACCATAGACACGGGATATGATTCCCAGCCCCAGGATGTCCTGGGCATCAGG
  CAGCTGGAAAGGCCCCTGCCCCTCACCTCCGTGTGTTACCCCCAGGACCTCCCCAGACCTCTCAGGTCCA
  GGGAGTTCCCTCAGTTTGAACCTCAGAGGTATCCAGCATGTGCACAGATGCTGCCTCCCAATCTTTCCCC
  ACATGCTCCATGGAACTATCATTACCATTGTCCTGGAAGTCCCGATCACCAGGTGCCATATGGCCATGAC
  TACCCTCGAGCAGCCTACCAGCAAGTGATCCAGCCGGCTCTGCCTGGGCAGCCCCTGCCTGGAGCCAGTG
  TGAGAGGCCTGCACCCTGTGCAGAAGGTTATCCTGAATTATCCCAGCCCCTGGGACCAAGAAGAGAGGCC
  CGCACAGAGAGACTGCTCCTTTCCGGGGCTTCCAAGGCACCAGGACCAGCCACATCACCAGCCACCTAAT
  AGAGCTGGTGCTCCTGGGGAGTCCTTGGAGTGCCCTGCAGAGCTGAGACCACAGGTTCCCCAGCCTCCGT
  CCCCAGCTGCTGTGCCTAGACCCCCTAGCAACCCTCCAGCCAGAGGAACTCTAAAAACAAGCAATTTGCC
  AGAAGAATTGCGGAAAGTCTTTATCACTTATTCGATGGACACAGCTATGGAGGTGGTGAAATTCGTGAAC
  TTTTTGTTGGTAAATGGCTTCCAAACTGCAATTGACATATTTGAGGATAGAATCCGAGGCATTGATATCA
  TTAAATGGATGGAGCGCTACCTTAGGGATAAGACCGTGATGATAATCGTAGCAATCAGCCCCAAATACAA
  ACAGGACGTGGAAGGCGCTGAGTCGCAGCTGGACGAGGATGAGCATGGCTTACATACTAAGTACATTCAT
  CGAATGATGCAGATTGAGTTCATAAAACAAGGAAGCATGAATTTCAGATTCATCCCTGTGCTCTTCCCAA
  ATGCTAAGAAGGAGCATGTGCCCACCTGGCTTCAGAACACTCATGTCTACAGCTGGCCCAAGAATAAAAA
  AAACATCCTGCTGCGGCTGCTGAGAGAGGAAGAGTATGTGGCTCCTCCACGGGGGCCTCTGCCCACCCTT
  CAGGTGGTTCCCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201106 representing NM_147686
  Red=Cloning site Green=Tags(s)
  
  MNRSIPVEVDESEPYPSQLLKPIPEYSPEEESEPPAPNIRNMAPNSLSAPTMLHNSSGDFSQAHSTLKLA
  NHQRPVSRQVTCLRTQVLEDSEDSFCRRHPGLGKAFPSGCSAVSEPASESVVGALPAEHQFSFMEKRNQW
  LVSQLSAASPDTGHDSDKSDQSLPNASADSLGGSQEMVQRPQPHRNRAGLDLPTIDTGYDSQPQDVLGIR
  QLERPLPLTSVCYPQDLPRPLRSREFPQFEPQRYPACAQMLPPNLSPHAPWNYHYHCPGSPDHQVPYGHD
  YPRAAYQQVIQPALPGQPLPGASVRGLHPVQKVILNYPSPWDQEERPAQRDCSFPGLPRHQDQPHHQPPN
  RAGAPGESLECPAELRPQVPQPPSPAAVPRPPSNPPARGTLKTSNLPEELRKVFITYSMDTAMEVVKFVN
  FLLVNGFQTAIDIFEDRIRGIDIIKWMERYLRDKTVMIIVAISPKYKQDVEGAESQLDEDEHGLHTKYIH
  RMMQIEFIKQGSMNFRFIPVLFPNAKKEHVPTWLQNTHVYSWPKNKKNILLRLLREEEYVAPPRGPLPTL
  QVVPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_147686
• ORF Size: 1695 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147686.1, NP_679211.1
• RefSeq Size: 2514 bp
• RefSeq ORF: 1698 bp
• Locus ID: 10758
• UniProt ID: O43734
• Cytogenetics: 6q21
• Gene Summary: This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Several alternative transcripts encoding different isoforms have been identified. Another transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene. [provided by RefSeq, Aug 2009]