Apolipoprotein L 1 (APOL1) (NM_003661) Human Tagged ORF Clone
Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, Myc-DDK-tagged
|Type||Human Tagged ORF Clone|
|Synonyms||APO-L; APOL; APOL-I; FSGS4|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Mammalian Cell Selection||None|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1194 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2856 bp|
|RefSeq ORF||1197 bp|
|Protein Families||Secreted Protein, Transmembrane|
|Gene Summary||This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]|
|cDNA Clone Resources|
|RC211114||APOL1 (Myc-DDK-tagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 1||
|RC211114L2||Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, mGFP tagged||
|RC211114L3||Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, Myc-DDK-tagged||
|RC211114L4||Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, mGFP tagged||
|RG211114||APOL1 (GFP-tagged) - Human apolipoprotein L, 1 (APOL1), transcript variant 1||
|SC109941||APOL1 (untagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 1||