Actc1 (NM_009608) Mouse Tagged ORF Clone
Lenti ORF clone of Actc1 (Myc-DDK-tagged) - Mouse actin, alpha, cardiac muscle 1 (Actc1)
|Product Name||Actc1 (NM_009608) Mouse Tagged ORF Clone|
|Type||Mouse Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1134 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1390 bp|
|RefSeq ORF||1134 bp|
|Cytogenetics||2 57.55 cM|
|Gene Summary||Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.[UniProtKB/Swiss-Prot Function]|
|cDNA Clone Resources|
|MC208103||Actc1 (untagged) - Mouse actin, alpha, cardiac muscle 1 (Actc1), (10ug)||
|MR225387||Actc1 (Myc-DDK-tagged) - Mouse actin, alpha, cardiac muscle 1 (Actc1)||
|MG225387||Actc1 (GFP-tagged) - Mouse actin alpha cardiac muscle 1 (Actc1), (10ug)||
|MR225387L4||Lenti ORF clone of Actc1 (mGFP-tagged) - Mouse actin, alpha, cardiac muscle 1 (Actc1)||