Kars (NM_053092) Mouse Tagged Lenti ORF Clone
CAT#: MR217682L3
- LentiORF®
Lenti ORF clone of Kars (Myc-DDK-tagged) - Mouse lysyl-tRNA synthetase (Kars), transcript variant 2
"NM_053092" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Kars |
Synonyms | AA589550; AL024334; AL033315; AL033367; D8Ertd698e; D8Wsu108e; LysRS; mKIAA0070 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR217682).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_053092 |
ORF Size | 1788 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053092.2, NP_444322.1 |
RefSeq Size | 2045 bp |
RefSeq ORF | 1788 bp |
Locus ID | 85305 |
UniProt ID | Q99MN1 |
Cytogenetics | 8 58.27 cM |
Gene Summary | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219549 | Kars (untagged) - Mouse lysyl-tRNA synthetase (Kars), transcript variant 2, (10ug) |
USD 610.00 |
|
MG217682 | Kars (tGFP-tagged) - Mouse lysyl-tRNA synthetase (Kars) transcript variant 2, (10ug) |
USD 755.00 |
|
MR217682 | Kars (Myc-DDK-tagged) - Mouse lysyl-tRNA synthetase (Kars), transcript variant 2 |
USD 555.00 |
|
MR217682L4 | Lenti ORF clone of Kars (mGFP-tagged) - Mouse lysyl-tRNA synthetase (Kars), transcript variant 2 |
USD 855.00 |
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