Abcc2 (NM_013806) Mouse Tagged ORF Clone

CAT#: MG227613

Abcc2 (tGFP-tagged) - Mouse ATP-binding cassette sub-family C (CFTR/MRP) member 2 (Abcc2), (10ug)

Product Images

Circular map for MG227613

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abcc2
• Synonyms: Abc30; AI173996; Cmoat; cMRP; Mrp2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227613 representing NM_013806
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAATTCTGCAACTCTACTTTTTGGAATCTCTCATTATTGAAAAGTCCGGAAGCCGACCTGCCTC
  TTTGTTTTGAACAAACTGTTCTTGTGTGGATTCCCTTGGGCTTTCTTTGGCTCTTGGCGCCTTGGCAACT
  TTACAGAATATACAGATCCAGGACCAAGAGATTTGCTATAACTAAATTCTACCTTGCCAAGCAGGTGTTC
  GTTGTGTGTCTTCTTATTTTAGCAGCCATAGACCTGTCTCTTGCACTCACAGAAGATACTGGACAAGCCA
  CAATTCCTCCTGTTAAATATACAAATCCAATTCTCTACCTATGCACTTGGCTCCTGGTGTTGGTGATCCA
  ACACTGCAGGCAATGCTGTATACAGAAGAATTCTTGGTTCCTGTCCATGTTCTGGATTCTCTCTCTATTA
  TGTGGCATATTCCAGTTTCAGACTCTGATACGAGCACTCCTGCAGGATAGCAAGTCCAACATGACCTATT
  CCTGCCTGTTCTTCGTCTCCTATGGTTTCCAGATTGTCATTTTGATCCTTTCAGCCTTTTCAGAATCAAG
  CGACTCAACACACGCCCCATCAGCTACAGCTTCATTTCTGAGTAGCGTTACGTTTAGTTGGTATGACAGC
  ACTGTTCTGAAAGGTTACAAGCATCCATTGACAATAGAAGATGTGTGGGATATTGAAGAAAATCTTAAAG
  CCAAGTCACTCACCAGCAAGTTTAAGACGATCATGACAAAGGACCTGCAGAAAGCCAGGCAGGCTCTTCA
  GAGGCGGCTGAAGAAGTCCCAGCAGAGCCCTGAGGGCACGTCACACGGATTGACCAAGAAGCAGAGTCAG
  AGCCAAGATGTCCTTGTCCTGGAAGACTCGAAAAAGAAGAAAAAGAAGTCTGAGGCCACCAAAGACTTTC
  CCAAATCCTGGTTGGTGAAGGCTCTCTTCAAAACGTTCTACGTGGTGATCCTGAAGTCATTTATTCTGAA
  ATTAGCACATGATATTCTTTTGTTTCTGAATCCTCAGCTGCTGAAATTTCTCATTGGCTTCGTGAAAGAC
  CCTGACTCATATCCGTGGGTTGGATATATCTATGCAATCCTAATGTTCTCTGTGACTCTCATCCAGTCTT
  TCTTCCTTCAGTGTTATTTTCAATTTTGTTTTGTGTTGGGAATGACTGTACGGACAACCATCATAGCTTC
  CGTATATAAGAAGGCACTAACCCTATCTAACTTGGCTAGGAGGCAGTACACGATTGGAGAGACGGTGAAC
  TTGATGTCTGTAGATTCCCAGAAGCTAATGGACGTGACCAACTACATACACTTGCTGTGGTCCAGTGTTT
  TACAGATTGCTTTGTCCATTTTCTTCCTGTGGAGAGAGTTGGGACCTTCGATTTTAGCAGGTGTTGGGCT
  TATGGTTCTCCTGGTCCCAGTTAATGGCGTCCTGGCTACCAAGATCAGGAAAATTCAGGTCCAGAATATG
  AAGAATAAAGACAAACGCTTAAAAATCATGAATGAGATTCTTAGTGGGATTAAGATCCTGAAATACTTTG
  CCTGGGAACCTTCCTTTAAAGAGCAAGTGAACAGCATTCGGAAGAAAGAACTCAGGAACTTGCTGCGCTT
  CAGCCAGCTGCAGACGATTTTGATTTTCATCTTACACCTAACTCCAACCCTGGTGTCCGTGATCACATTT
  TCTGTTTACGTCCTGGTGGATAGTCAAAATGTTTTGAACGCGGAGAAGGCTTTCACCTCCATCACCCTCT
  TCAATATCCTGCGCTTTCCTCTGGCCATGCTTCCCATGGTGATCTCTTCAGTCATCCAGGCCAGCGTCTC
  TGTAGACCGGCTGGAGCAGTACCTGGGAAGCGATGATCTGGACTTGTCTGCCATTCGCCATGTCTGCCAT
  TTTGATAAAGCTGTGCAGTTCTCAGAAGCCTCTTTTACTTGGGACCGGGACTTGGAAGCCACAATCCAAG
  ATGTGAACCTGGACATAAAACCAGGCCAACTGGTGGCTGTGGTGGGCACTGTAGGCTCTGGGAAGTCCTC
  TTTGATATCAGCCATGCTGGGAGAAATGGAGAATGTCCATGGGCATATCACCATCAAGGGCTCCATAGCC
  TACGTCCCTCAGCAGGCCTGGATTCAGAACGGGACCATCAAAGATAACATCCTGTTTGGGTCAGAATACG
  ATGAAAAGAAGTACCAGCGAGTTATCGAAGCGTGCGCTCTCCTCCCAGACTTGGAAATGTTGCCTGGAGG
  AGACATGGCTGAGATCGGAGAGAAGGGTATAAATCTCAGTGGCGGTCAGAAGCATCGAGTCAGCCTGGCC
  CGGGCCACCTATCAAGATGCAGACATCTATATTCTAGACGACCCCCTGTCGGCTGTGGACACTCACGTGG
  GAAAACACATTTTCAACAAGGTTGTGGGCCCCAATGGCCTGCTGAGCGGCAAGACTCGGATCTTGGTTAC
  ACATGGCATTCACTTCCTTCCCCAAGTGGATGAGATTGTCGTTCTGGGGAAAGGCACCATCTTAGAGAAG
  GGATCCTATAGTGACCTGATGGACAAGAAAGGAGTGTTCGCTAAGAACTGGAAGACATTCATGAAGCATT
  CGGGACCTGAAGGAGAGGCTACAGTCGATAACGACAGTGAGGAAGAGGATGGTGACTGTGGGCTGATCCC
  AACTGTGGAGGAAATTCCTGACGATGCAGCTTCCTTGACCATGAGAAGGGAGAACAGCCTTCGCCGAACA
  CTGAGCCGCAGCTCTAGGTCCGGCAGCAGGCGTGGGAAGTCCCTCAAAAGCTCCTTGAAAATTAAAAGTG
  TGAATGCCTTGAATAAGAAGGAGGAAGTAGTGAAAGGACAAAAACTAATTAAGAAGGAATTTGTGGAAAC
  TGGGAAGGTAAAGTTCTCCATCTACCTGAAATATCTGCAGGCGGTAGGATGGTGGTCCCTGCTCTTCATC
  GTCATCTTCTACGTATTGAACTATGTGGCTTTTATTGGCACTAACCTCTGGCTGAGTGCTTGGACCAGTG
  ACTCTGAAAAACAGAATGGGACCGACAATTCACCTTCTCAGAGGGACATGAGAATCGGGGTCTTTGGAGC
  TCTGGGAATAGCACAAGGTATATTTTTGCTTAGTTCAAGTCTATGGAGTATATATGCTTGCAGAAATGCA
  TCCAAAACTTTGCACAGGCAGCTGTTAACCAACATTCTCCGCGCACCCATGAGTTTTTTTGACACAACTC
  CCACAGGCCGGATTGTGAACAGATTTGCCGGGGATATTTCTACGGTGGACGACACACTCCCCCAGACTTT
  GCGAAGCTGGCTGCTGTGTTTCTTCGGCATAGTTAGCACTCTCGTGATGATCTGCATGGCCACCCCCATC
  TTCATCATCATCATCATCCCTCTCAGCATTCTCTATGTATCTGTGCAGGTGTTTTATGTGGCTACATCCC
  GCCAGCTGAGACGGCTGGATTCTGTCACCAAATCTCCGATCTATTCTCACTTTAGTGAGACTGTCTCTGG
  TTTGCCTGTTATTCGGGCCTTTGAGCACCAGCAGAGATTTCTAGCAAACAGTGAGAAGCAGATTGACACC
  AACCAGAAATGCGTCTTTTCCTGGATTACCTCCAACCGGTGGCTTGCCATTCGCCTGGAGCTGGTTGGAA
  ACCTGATTGTCTTCTGCTCGGCCTTGCTTCTGGTTATTTATAAGAATTCCTTAACCGGGGACACTGTGGG
  CTTTGTTCTGTCCAACGCCCTCAACATCACACAAACCCTGAACTGGCTAGTGAGGATGACGTCAGAAGTA
  GAGACCAACATCGTGGCTGTTGAGCGAATAAATGAATACATAAATGTGGATAATGAGGCGCCGTGGGTGA
  CCGACAAGAAGCCTCCGGCAGATTGGCCCAAAAAAGGTGAGATCCAGTTCAACAACTATCAAGTGCGGTA
  TCGGCCGGAGCTGGATCTGGTCCTGAAAGGGATTACTTGTAACATCAAGAGCACCGAGAAGGTCGGTGTG
  GTGGGCAGGACCGGAGCTGGAAAATCATCCCTCACAAACTGCCTCTTCAGAATCTTAGAGTCTGCAGGTG
  GCCAGATCATCATTGATGGGATTGATATTGCCTCCATTGGACTGCACGACCTTCGAGGGAGACTGACCAT
  CATTCCCCAGGACCCCATTTTGTTCTCTGGGAATCTGAGGATGAATCTCGACCCTTTCAACAAATACTCG
  GATGAGGAGATCTGGAGGGCCCTGGAATTGGCTCATCTCAAATCCTTTGTGGCTGGCCTGCAACTTGGGT
  TGCTCCATGAAGTGACAGAGGGCGGTGACAACCTGAGCATAGGGCAGAGGCAGCTCCTATGCCTGGGTAG
  GGCCGTGCTTCGAAAATCCAAAATCCTGGTCCTGGACGAAGCCACAGCCGCAGTGGATCTAGAGACGGAT
  AGCCTCATTCAGACGACCATCCGGAACGAGTTCTCCCAGTGCACGGTCATCACTATCGCACACAGGCTGC
  ACACCATCATGGACAGTGACAAGATAATGGTCCTAGACAGCGGCAAGATTGTTGAATACGGCAGTCCTGA
  AGAACTGCTGTCCAATATGGGTCCCTTCTACTTGATGGCCAAGGAAGCCGGCATTGAAAGTGTGAACCAC
  ACGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227613 representing NM_013806
  Red=Cloning site Green=Tags(s)
  
  MDEFCNSTFWNLSLLKSPEADLPLCFEQTVLVWIPLGFLWLLAPWQLYRIYRSRTKRFAITKFYLAKQVF
  VVCLLILAAIDLSLALTEDTGQATIPPVKYTNPILYLCTWLLVLVIQHCRQCCIQKNSWFLSMFWILSLL
  CGIFQFQTLIRALLQDSKSNMTYSCLFFVSYGFQIVILILSAFSESSDSTHAPSATASFLSSVTFSWYDS
  TVLKGYKHPLTIEDVWDIEENLKAKSLTSKFKTIMTKDLQKARQALQRRLKKSQQSPEGTSHGLTKKQSQ
  SQDVLVLEDSKKKKKKSEATKDFPKSWLVKALFKTFYVVILKSFILKLAHDILLFLNPQLLKFLIGFVKD
  PDSYPWVGYIYAILMFSVTLIQSFFLQCYFQFCFVLGMTVRTTIIASVYKKALTLSNLARRQYTIGETVN
  LMSVDSQKLMDVTNYIHLLWSSVLQIALSIFFLWRELGPSILAGVGLMVLLVPVNGVLATKIRKIQVQNM
  KNKDKRLKIMNEILSGIKILKYFAWEPSFKEQVNSIRKKELRNLLRFSQLQTILIFILHLTPTLVSVITF
  SVYVLVDSQNVLNAEKAFTSITLFNILRFPLAMLPMVISSVIQASVSVDRLEQYLGSDDLDLSAIRHVCH
  FDKAVQFSEASFTWDRDLEATIQDVNLDIKPGQLVAVVGTVGSGKSSLISAMLGEMENVHGHITIKGSIA
  YVPQQAWIQNGTIKDNILFGSEYDEKKYQRVIEACALLPDLEMLPGGDMAEIGEKGINLSGGQKHRVSLA
  RATYQDADIYILDDPLSAVDTHVGKHIFNKVVGPNGLLSGKTRILVTHGIHFLPQVDEIVVLGKGTILEK
  GSYSDLMDKKGVFAKNWKTFMKHSGPEGEATVDNDSEEEDGDCGLIPTVEEIPDDAASLTMRRENSLRRT
  LSRSSRSGSRRGKSLKSSLKIKSVNALNKKEEVVKGQKLIKKEFVETGKVKFSIYLKYLQAVGWWSLLFI
  VIFYVLNYVAFIGTNLWLSAWTSDSEKQNGTDNSPSQRDMRIGVFGALGIAQGIFLLSSSLWSIYACRNA
  SKTLHRQLLTNILRAPMSFFDTTPTGRIVNRFAGDISTVDDTLPQTLRSWLLCFFGIVSTLVMICMATPI
  FIIIIIPLSILYVSVQVFYVATSRQLRRLDSVTKSPIYSHFSETVSGLPVIRAFEHQQRFLANSEKQIDT
  NQKCVFSWITSNRWLAIRLELVGNLIVFCSALLLVIYKNSLTGDTVGFVLSNALNITQTLNWLVRMTSEV
  ETNIVAVERINEYINVDNEAPWVTDKKPPADWPKKGEIQFNNYQVRYRPELDLVLKGITCNIKSTEKVGV
  VGRTGAGKSSLTNCLFRILESAGGQIIIDGIDIASIGLHDLRGRLTIIPQDPILFSGNLRMNLDPFNKYS
  DEEIWRALELAHLKSFVAGLQLGLLHEVTEGGDNLSIGQRQLLCLGRAVLRKSKILVLDEATAAVDLETD
  SLIQTTIRNEFSQCTVITIAHRLHTIMDSDKIMVLDSGKIVEYGSPEELLSNMGPFYLMAKEAGIESVNH
  TEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013806
• ORF Size: 4629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013806.2, NP_038834.2
• RefSeq Size: 5389 bp
• RefSeq ORF: 4632 bp
• Locus ID: 12780
• UniProt ID: Q8VI47
• Cytogenetics: 19 36.67 cM
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the canalicular surface of the hepatocyte and in biliary transport, and appears to contribute to drug resistance in mammalian cells. Several different mutations in the human gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. Alternative splice variants have been observed for this gene; however, they have not been fully described. [provided by RefSeq, Jul 2008]