Il1r1 (NM_008362) Mouse Tagged ORF Clone

CAT#: MG227508

Il1r1 (tGFP-tagged) - Mouse interleukin 1 receptor type I (Il1r1) transcript variant 1, (10ug)

Product Images

Circular map for MG227508

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Il1r1
• Synonyms: CD121a; CD121b; IL-1R1; IL-iR; Il1r-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227508 representing NM_008362
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATATGAAAGTGCTACTGGGGCTCATTTGTCTCATGGTGCCTCTGCTGTCGCTGGAGATTGACG
  TATGTACAGAATATCCAAATCAGATCGTTTTGTTTTTATCTGTAAATGAAATTGATATTCGCAAGTGTCC
  TCTTACTCCAAATAAAATGCACGGCGACACCATAATTTGGTACAAGAATGACAGCAAGACCCCCATATCA
  GCGGACCGGGACTCCAGGATTCATCAGCAGAATGAACATCTTTGGTTTGTACCTGCCAAGGTGGAGGACT
  CAGGATATTACTATTGTATAGTAAGAAACTCAACTTACTGCCTCAAAACTAAAGTAACCGTAACTGTGTT
  AGAGAATGACCCTGGCTTGTGTTACAGCACACAGGCCACCTTCCCACAGCGGCTCCACATTGCCGGGGAT
  GGAAGTCTTGTGTGCCCTTATGTGAGTTATTTTAAAGATGAAAATAATGAGTTACCCGAGGTCCAGTGGT
  ATAAGAACTGTAAACCTCTGCTTCTTGACAACGTGAGCTTCTTCGGAGTAAAAGATAAACTGTTGGTGAG
  GAATGTGGCTGAAGAGCACAGAGGGGACTATATATGCCGTATGTCCTATACGTTCCGGGGGAAGCAATAT
  CCGGTCACACGAGTAATACAATTTATCACAATAGATGAAAACAAGAGGGACAGACCTGTTATCCTGAGCC
  CTCGGAATGAGACGATCGAAGCTGACCCAGGATCAATGATACAACTGATCTGCAACGTCACGGGCCAGTT
  CTCAGACCTTGTCTACTGGAAGTGGAATGGATCAGAAATTGAATGGAATGATCCATTTCTAGCTGAAGAC
  TATCAATTTGTGGAACATCCTTCAACCAAAAGAAAATACACACTCATTACAACACTTAACATTTCAGAAG
  TTAAAAGCCAGTTTTATCGCTATCCGTTTATCTGTGTTGTTAAGAACACAAATATTTTTGAGTCGGCGCA
  TGTGCAGTTAATATACCCAGTCCCTGACTTCAAGAATTACCTCATCGGGGGCTTTATCATCCTCACGGCT
  ACAATTGTATGCTGTGTGTGCATCTATAAAGTCTTCAAGGTTGACATAGTGCTTTGGTACAGGGACTCCT
  GCTCTGGTTTTCTTCCTTCAAAAGCTTCAGATGGAAAGACATACGATGCCTATATTCTTTATCCCAAGAC
  CCTGGGAGAGGGGTCCTTCTCAGACTTAGATACTTTTGTTTTTAAACTGTTGCCTGAGGTCTTGGAGGGA
  CAGTTTGGATACAAGCTGTTCATTTATGGAAGGGATGACTATGTTGGAGAAGATACCATCGAGGTTACTA
  ATGAAAATGTAAAGAAAAGCAGGAGGCTGATTATCATTCTAGTGAGAGATATGGGAGGCTTCAGCTGGCT
  GGGCCAGTCATCTGAAGAGCAAATAGCCATATACAATGCTCTCATCCAGGAAGGAATTAAAATCGTCCTG
  CTTGAGTTGGAGAAAATCCAAGACTATGAGAAAATGCCAGATTCTATTCAGTTCATTAAGCAGAAACACG
  GAGTCATTTGCTGGTCAGGAGACTTTCAAGAAAGACCACAGTCTGCAAAGACCAGGTTCTGGAAAAACTT
  AAGATACCAGATGCCAGCCCAACGGAGATCACCATTGTCTAAACACCGCTTACTAACCCTGGATCCTGTG
  CGGGACACTAAGGAGAAACTGCCGGCAGCAACACACTTACCACTCGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227508 representing NM_008362
  Red=Cloning site Green=Tags(s)
  
  MENMKVLLGLICLMVPLLSLEIDVCTEYPNQIVLFLSVNEIDIRKCPLTPNKMHGDTIIWYKNDSKTPIS
  ADRDSRIHQQNEHLWFVPAKVEDSGYYYCIVRNSTYCLKTKVTVTVLENDPGLCYSTQATFPQRLHIAGD
  GSLVCPYVSYFKDENNELPEVQWYKNCKPLLLDNVSFFGVKDKLLVRNVAEEHRGDYICRMSYTFRGKQY
  PVTRVIQFITIDENKRDRPVILSPRNETIEADPGSMIQLICNVTGQFSDLVYWKWNGSEIEWNDPFLAED
  YQFVEHPSTKRKYTLITTLNISEVKSQFYRYPFICVVKNTNIFESAHVQLIYPVPDFKNYLIGGFIILTA
  TIVCCVCIYKVFKVDIVLWYRDSCSGFLPSKASDGKTYDAYILYPKTLGEGSFSDLDTFVFKLLPEVLEG
  QFGYKLFIYGRDDYVGEDTIEVTNENVKKSRRLIIILVRDMGGFSWLGQSSEEQIAIYNALIQEGIKIVL
  LELEKIQDYEKMPDSIQFIKQKHGVICWSGDFQERPQSAKTRFWKNLRYQMPAQRRSPLSKHRLLTLDPV
  RDTKEKLPAATHLPLG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008362
• ORF Size: 1728 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008362.2, NP_032388.1
• RefSeq Size: 4739 bp
• RefSeq ORF: 1731 bp
• Locus ID: 16177
• UniProt ID: P13504
• Cytogenetics: 1 18.81 cM
• Gene Summary: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity).[UniProtKB/Swiss-Prot Function]