Caps2 (NM_178278) Mouse Tagged ORF Clone

CAT#: MG227027

Caps2 (tGFP-tagged) - Mouse calcyphosphine 2 (Caps2), (10ug)

Product Images

Circular map for MG227027

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Caps2
• Synonyms: D630005B03Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227027 representing NM_178278
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCCACCACTTGATCTGGGAAGCCTTGTTGACTCTGATGAGGAGGATAATTTTTCACAAACACCAC
  GTGGTACAGTCCATGTTCATCTTAACCCACCGCGGTCACAGCCTGCTCTGGGCTGGGTTCTGCCATGTCA
  AAGACCTGACAGTCAGCATCGACTACAGGAAGTGGAACAGGACGTAATACCTGAGGATTTGCCAGCGCCA
  ACAGGCAAATACAGGCTAAAGTACCAGCAGTACGCAAGTGAAATGAAAGACGGCTACAAGCAGTATATTC
  AGAGGAGTACAGAGAAGCCAAAGGCAGCCAGCAGGCCAGAGGCAACCGAGAAGGTGGAAGGAGCCAGTCT
  GGATGACCTCATGACTTTGGATAGGAAGGCCCTCTTACAGCAAGGTTACGCAGATAGCCCTTACGGTAGA
  CAGAGCATCACGAGGAAATCAGATGTGGAGACTGTGGCTATAGAGAAGAAGAAACAGACCGTTGCAGAGC
  AGATGATGATGGACCACTTATCCAGGGCTGTCATCAGCGACCCAGAACAAGATTTAAACACCAAGAACCA
  AGAGAGTTCTCGTGTCCCTCCAGACTCAGAGAGGGCGCCACTTCGCGTCAGGAGACGAACTCTGCATGAA
  ACGAAGATAAGAACCAACTCTGCATTAACAGAGAATGACCTTTCTCAGAAAGTAGAATTTGACGGGAGGG
  TCTTATCAAGAAATGGACGCGATGCCTGCCGGGAACTAATTGGGTTTTTCTTTGCCCACGACCAGTCCCT
  AACTGTGTATGAATATCGGATGTTCGGGAAAAACAGAACAAGTGTGCTTCCTTTTATTAAAAAAGACATT
  TATCATCATCAGTGTGGACGAAGAAAAGGAAAGCAATATGAGCTTGGTGACGTCTATACTGGTGCAACTT
  TGACATTTTTGAGCTGTGATCAACCTAGCCTTCCAAAGACCATAAAAGAAAATGCATTGCTTAGACTTCG
  AATTACAAATATTGATCAAGTAGCTTTGAATTCTCTGAAAGCTGCCTCTGCGGAACATGGGGAAGAAGAG
  GCAGTCAGTCCGGAGGCTCATGATCAGTTGGTGCTACAGGCCATTCAGGATAAATTGAAAGAACAACTGC
  ACAAGAAGGGTGCTCGTATTTTGACTGGATTGGGAAGATACTTCCAAGGTTTGGACAAGGAAGGGAATGG
  GCTTTTAGAAAAGGCAGATTTTCAGCAAGCTCTGAAAACCTTTCACTTGGAAGTGTCTGAACAGGACTTT
  GAGTCTTTCTGGCTAATTCTGCAAGGATATGGCCATAGCAAGAACAAGGTTGACTATGGAGAATTCAAAC
  GTGCCATTTTTGGTGAAATGAATGAATATAGGAAGTCATTTGTTCGCAAGGCCTTCATGCAACTGGATTT
  TAACAAAACCGGCATTGTGTCTGTAATAGACATAAGAAAGTGTTACTGCGCCAAGAAGCATCCACGTGTG
  ATCTCAGGGCATTCAACAGAGGAGGAAATCAAATCATCTTTCCTAGAGACGTTAAAAGGCACCTGCAGCA
  AGTGCGATGAAGTTTCATACGGAGAATTCGAAGATTACTATGAAGGCCTAAGTATAGGGGTAGCAGGCGA
  TGAAGACTTTGTCAACATCTTACGTATTCCGTGGAGAATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227027 representing NM_178278
  Red=Cloning site Green=Tags(s)
  
  MVPPLDLGSLVDSDEEDNFSQTPRGTVHVHLNPPRSQPALGWVLPCQRPDSQHRLQEVEQDVIPEDLPAP
  TGKYRLKYQQYASEMKDGYKQYIQRSTEKPKAASRPEATEKVEGASLDDLMTLDRKALLQQGYADSPYGR
  QSITRKSDVETVAIEKKKQTVAEQMMMDHLSRAVISDPEQDLNTKNQESSRVPPDSERAPLRVRRRTLHE
  TKIRTNSALTENDLSQKVEFDGRVLSRNGRDACRELIGFFFAHDQSLTVYEYRMFGKNRTSVLPFIKKDI
  YHHQCGRRKGKQYELGDVYTGATLTFLSCDQPSLPKTIKENALLRLRITNIDQVALNSLKAASAEHGEEE
  AVSPEAHDQLVLQAIQDKLKEQLHKKGARILTGLGRYFQGLDKEGNGLLEKADFQQALKTFHLEVSEQDF
  ESFWLILQGYGHSKNKVDYGEFKRAIFGEMNEYRKSFVRKAFMQLDFNKTGIVSVIDIRKCYCAKKHPRV
  ISGHSTEEEIKSSFLETLKGTCSKCDEVSYGEFEDYYEGLSIGVAGDEDFVNILRIPWRI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178278
• ORF Size: 1776 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178278.4, NP_840062.2
• RefSeq Size: 2760 bp
• RefSeq ORF: 1779 bp
• Locus ID: 353025
• UniProt ID: Q8BUG5
• Cytogenetics: 10 D2