Dand5 (NM_201227) Mouse Tagged ORF Clone

CAT#: MG226975

Dand5 (tGFP-tagged) - Mouse DAN domain family member 5 (Dand5) transcript variant 1, (10ug)

Product Images

Circular map for MG226975

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dand5
• Synonyms: Cerl-2; Cerr2; coco; Dte; GREM3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226975 representing NM_201227
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGTAGCCAGTTCACCACGCTGCTGGGTCTGTTCAGTGGGGCCTGGCTGCCCACAGGCTCAGGGA
  GGCCTGGGGCCCCAGCGACCCCTGTTCAGTCCGGGACTGCTATCAATCAGAGCTGGACTCTGGATCCCCT
  GGTGCCCATCTCTGCCCTGGGTAGCTGGGAGGCCTTCCTGGGCCTGCAGAACAAACAGCAGGGGACAGGT
  GAGCTGCAGGGAGGAGGGCAGAGAGTAGCTGCTGGTGTGCCTTTGCCCTTGGCTCCTCAAGAAGTGCTTC
  AGGAGACTTGTAAAGCTCTGTCCTTTGTTCAGGTGATCTCCAGGCCTGGTTGCACAAGTGCCCGGGTCCT
  TAATCATCTCTGTTTTGGCCGCTGTTCCTCCTTCTACATTCCCAGCTCAGATCCCACCCCTGTAGTCTTC
  TGCAACAGCTGTGTGCCGGCTCGAAAGCGCTGGACATCGGTGACGCTGTGGTGTGGAGCTGGCCAATTAG
  CCTCCCCTCGGCGGGTGAGGATTTCCACGGTATTGGTCCAGAAGTGTCAGTGCCGCCCGAAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226975 representing NM_201227
  Red=Cloning site Green=Tags(s)
  
  MFRSQFTTLLGLFSGAWLPTGSGRPGAPATPVQSGTAINQSWTLDPLVPISALGSWEAFLGLQNKQQGTG
  ELQGGGQRVAAGVPLPLAPQEVLQETCKALSFVQVISRPGCTSARVLNHLCFGRCSSFYIPSSDPTPVVF
  CNSCVPARKRWTSVTLWCGAGQLASPRRVRISTVLVQKCQCRPKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201227
• ORF Size: 555 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201227.1
• RefSeq Size: 1584 bp
• RefSeq ORF: 558 bp
• Locus ID: 23863
• UniProt ID: Q76LW6
• Cytogenetics: 8 C2
• Gene Summary: Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, and tissue growth and differentiation.[UniProtKB/Swiss-Prot Function]