Cacna1c (NM_009781) Mouse Tagged ORF Clone

CAT#: MG226770

Cacna1c (tGFP-tagged) - Mouse calcium channel voltage-dependent L type alpha 1C subunit (Cacna1c) transcript variant 1, (10ug)

Product Images

Circular map for MG226770

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cacna1c
• Synonyms: Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226770 representing NM_009781
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCGGGCCTTTGTTCAGCCATCCACACCCCCATACCAGCCACTCTCCAGTCACTCGTCTGAGGAAA
  CAGAGAGGAAGTTCAAGGGCAAGGTGGTACATGAAGCTCAACTCAACTGTTTCTACATTTCTCCTGGAGG
  TTCCAACTATGGGAGCCCACGCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCC
  GAGCACATCCCTACTCCAGGGGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGC
  TCATGGGCAGTGCTGGCAACGCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGG
  GAAACCCAAGAAGCAGGGGGGCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAG
  AACCCTATCCGGAGGGCGTGCATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTA
  TTTTTGCCAATTGTGTGGCCTTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTC
  CAACCTGGAACGAGTGGAGTATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCC
  TACGGACTTCTCTTCCACCCCAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGG
  TTGTAGGGCTTTTTAGTGCAATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAA
  AGGAGCTGGATTCGACGTGAAGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGA
  GTCCCAAGTCTCCAGGTGGTCCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTC
  TTGTGCTCTTCGTCATCATCATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGAC
  CTGCTACAACCAGGAGGGCATAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACA
  GGCCATGGGCGACAGTGTCAGAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCA
  CCAACTTCGACAACTTCGCCTTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGA
  CGTGCTGTACTGGATGCAAGACGCTATGGGCTATGAGTTGCCCTGGGTGTATTTTGTCAGTCTGGTCATC
  TTTGGATCCTTTTTCGTTCTAAATCTGGTTCTCGGTGTTTTGAGCGGGGAGTTTTCCAAAGAGAGGGAGA
  AAGCCAAAGCCCGAGGAGATTTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTA
  CCTGGACTGGATCACCCAGGCAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAG
  CCTCGAAACATGAGCATGCCCACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGGTGACA
  TCGAGGGAGAAAACTGTGGAGCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTACTGGCG
  CAGGTGGAATCGATTCTGCAGAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGCTCGTG
  ATCTTCCTGGTGTTCCTCAACACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCTCACAG
  AAGTGCAAGACACAGCCAATAAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATGTACAG
  CCTGGGTCTTCAGGCCTATTTTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGGGCATC
  CTGGAGACCATCCTGGTGGAGACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGT
  TGCTCAGGATCTTCAAGATCACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTGAACTC
  AGTGCGCTCCATTGCCTCCCTGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATG
  CAGCTCTTTGGAGGGAAGTTCAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTTCCCGC
  AGTCTCTCCTCACTGTGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCAT
  GGCTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGA
  AATTATATCCTACTGAATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCT
  CAGCCCAAAAGGAGGAGGAAGAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAA
  ACAGGAGGTGATGGAGAAGCCAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCATTACA
  GCCGATGGAGAATCCCCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGAGGATA
  AGAGTCCCCACTCCAACCCAGACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTGGGGCC
  ACGCCCCCGGCCCCTGTCTGAGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTGCATTT
  TTCATCTTCAGCCCAAACAACAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTTCACCA
  ACCTCATCCTCTTCTTCATTCTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCACACCTC
  CTTCAGGAACCATATCCTAGGCAATGCAGACTATGTCTTCACTAGTATCTTTACATTAGAAATTATCCTT
  AAGATGACTGCTTACGGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACC
  TGCTGGTGGTTAGCGTGTCCCTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTT
  ACGAGTGCTGCGAGTCCTCAGACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAG
  TGCGTGTTTGTGGCCATCCGGACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCG
  CCTGCATTGGGGTCCAGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGC
  AGAATGCAAGGGTAACTATATCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGC
  TGGGAGAACAGCAAGTTTGACTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCT
  TCGAAGGGTGGCCAGAGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAA
  CTACCGTGTGGAGATCTCCATCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATC
  TTCGTGGGTTTCGTCATTGTCACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACA
  AGAACCAGAGACAATGTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCA
  GCACCAGTACAAAGTGTGGTACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTG
  CTCAACACCATCTGCCTGGCCATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATAC
  TCAACATGCTTTTCACCGGCCTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGG
  TTACTTTAGTGATCCCTGGAATGTTTTTGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCATTCTC
  AGTGAGACTAATCCAGCTGAACATACCCAATGCTCTCCCTCTATGAGTGCAGAGGAGAACTCCCGCATCT
  CCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGGAAGGCATCCG
  AACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGATTGTGATGCTG
  TTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCCCTGAATGACACCACAGAGATCA
  ATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCACCGGAGAGGC
  CTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCCCAGCAACAGC
  ACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCTCTGTG
  CCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACTACCTGACTAGGGATTGGTC
  TATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAGTATGACCCTGAAGCCAAGGGT
  CGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTTGGGAAATTGT
  GTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGAACAGCGATGGCACAGTCAT
  GTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAAAACAGAAGGGAACCTAGAGCAA
  GCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACTAGCATGAAGCTGTTGGACCAGG
  TGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATGCCACCTTCCTGATCCAAGAGTA
  CTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAATGCACTGTCC
  CTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGGCGGGCCATCTCTGGGGATCTGA
  CTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATGACATCTTCAG
  GAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGACAGCAGGGGCAACTTTCCTCAG
  ACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAACCAAGCTGACACTGAGTCACCGT
  CCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCATCCACGGGCTCCAATGCCAACAT
  CAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGGCTACTCCAGCACGGTCAGCACT
  GTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAGCTCAGCTCTA
  AGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGATATTTCCAGATGAGACCCGCAG
  CGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCTCTCCACAGATATGTTCTCCTAC
  CAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCATCTCCAAAGA
  GGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAAAGCGACAAAA
  GGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGTTCATCATCAGGCATTGGCA
  GTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACATTCCCCAGGCCGTGCCCCACAC
  CCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTACCCTACGGCTGGAGGGGGCAGA
  GTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTCCTGGTCTGAGGAGACGACAGCC
  TGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGCCAGGCTGGAG
  CTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAGAAGGACTGGG
  ACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGCTGACGCCTGCGACATGACA
  ATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCCAACGGCACCC
  TCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACGAGAGCTGCGC
  ATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAGCAACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226770 representing NM_009781
  Red=Cloning site Green=Tags(s)
  
  MIRAFVQPSTPPYQPLSSHSSEETERKFKGKVVHEAQLNCFYISPGGSNYGSPRPAHANMNANAAAGLAP
  EHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGGTTATRPPRALLCLTLK
  NPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIA
  YGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSG
  VPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALET
  GHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVI
  FGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDK
  PRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLV
  IFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGI
  LETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGM
  QLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICG
  NYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSIT
  ADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAF
  FIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIIL
  KMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQ
  CVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRS
  WENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNI
  FVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLIL
  LNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVIL
  SETNPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVML
  FFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNS
  TEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKG
  RIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQ
  ANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALS
  LQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVTYYQSDSRGNFPQ
  TFATQRPLHINKTGNNQADTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRFPHPAGYSSTVST
  VEGHGPPLSPAVRVQEAAWKLSSKRCHSRESQGATVNQEIFPDETRSVRMSEEAEYCSEPSLLSTDMFSY
  QEDEHRQLTCPEEDKREIQPSPKRSFLRSASLGRRASFHLECLKRQKDQGGDISQKTALPLHLVHHQALA
  VAGLSPLLQRSHSPTTFPRPCPTPPVTPGSRGRPLRPIPTLRLEGAESSEKLNSSFPSIHCSSWSEETTA
  CSGSSSMARRARPVSLTVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMT
  IEEMENAADNILSGGAQQSPNGTLLPFVNCRDPGQDRAVAPEDESCAYALGRGRSEEALADSRSYVSNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009781
• ORF Size: 6507 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009781.4, NP_033911.2
• RefSeq Size: 8301 bp
• RefSeq ORF: 6510 bp
• Locus ID: 12288
• Cytogenetics: 6 55.86 cM
• Gene Summary: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]