Fam120c (NM_198105) Mouse Tagged ORF Clone

CAT#: MG226693

Fam120c (tGFP-tagged) - Mouse family with sequence similarity 120 member C (Fam120c), (10ug)

Product Images

Circular map for MG226693

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fam120c
• Synonyms: D930001I21Rik; ORF34
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226693 representing NM_198105
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGTCCAGGGCTTCCAAGAGTTCCTGGAGAAGCGCTGTCCCGGGGCTGTGGTGCCTGTGGACCTCC
  TCAAACTCGCGCGCACCGTCTCGCGCCAGCAGCAGCAGCAGCACCTGCACCGCCAGCTGCCGCCAGCAGC
  CCTAGCGCCAGGGGCTCCACGCATCACCAGGGGCTCTGCTCCTCTGCCGCCGCCGCCGCTCCCGCCCGCT
  GCCTTTGGTGCTTACTCCGGGGGCGCGGGGCCGTCTCGGCACCATCACCCTGCTCACCATTTCCACCACC
  ATGGCCAAGCGCCGCCAGGGCTGCATCCGCCGCCGCCCCCTCCGCTGCCTGGGGCTCGGGTATTGGTAGA
  CGCGGGCTCGGCGCTGCCGCGGCTCTATGGTGGCTACCAGACGGATTGGGTTTGTGGCGGCCAGTGGAAT
  GCCATGCTGGGCTACTTGTCAGCGCTGTGCCAGGCTTGTGCCTATCCGGGCGGAGACGGCCTGGAGCTGG
  TGGTCATGTTCCCTGGGGGCCTGGGGAAGGACCGACTGGCCGAGTGGGGCCGTCGGTGCCAGGCGGAGCG
  GCAGACTGCGCAACTGATCGTGGGACACGTGGGCAACAAGGGCACCCCTCCACCGCGGGCCTGGTTCCTG
  CCACCTGCCTGTCTGAGCCACTGCGTGAGGCTAGCACTCATCCGCTTCCGGGTCAAGGTCTTTCAGAGCC
  TTGAAGACCACCATTTGGAAGTGGTGGCTTTTTTCAGAGAGAATGGCTTTCATGGTCTTCTTGCTCACGA
  CTCTGAGTATGCGCTTTACAATATTCCATCTTACTACAGTTCCCATGCGCTGAAGCTGAGCTGGAATGGC
  AAGAACCTTACAACCAACCAGTTCCTGATGCAGGAAGTGGCCAAGCAGCTGGGTTTGAAGCGAATGAATT
  TTCCCATATTTGCTGCACTGCTTGGTAACCACATTCTACCGGATGAAGACTTGGCTGCATTTCACTGGAG
  CCTTTTGGGACCAGAACATCCTCTTGCATCACTTAAGGTCCGAGCTCATCAGCTGGTTCTTCCCCCTTGT
  GACGTCGTGATCAAGGCTGTTTCTGAGTATGTTAGTTCCATCAAAGACCCCTCAAACCTGGATGTGGTTG
  GCAAGGATGTCTTCAAACAATCTCAGTCTAGGACAGAAGACAAAATAGAACGATTCAAGAAAGCAGTTGA
  GTACTATTCAGTCACAACCAAACTCTCTTCACTGCCAGTGGGTCCTTCCTTTCTAGGCTTTCGAAATAAT
  CGACTTGGAAATCCTCCCCTTCCACGGAATCAGATGGGCCCTATTTCTCCTGGAAAGCCAATGTTTTCTC
  GCCAGGTGCCCCAGAAAATGAAATATCCACCACCATTTCCAATGGGACCCAACTCGTCTCTTCTCTTCTC
  CCATAGTGTGGGGGAATCCCATGCTTTCTCTGAGGATGCCATGCTGCAGGACAATTCCTTTGCCAATTGG
  GCTGTTTCTTATGACTCTAATACATCCCAGTTTCCTAACTGCCTGACTTCCAAAACTTCACCTCCTTTGG
  GACCAGATTCTTCCCATTCCTCTTCCTCTGATGGTGACGAGGCAAATGGAGCTGGTTCTGAACAAATCAC
  AGAAGCAGTTCAACAACAGCCTGGATGGGAAGATCCCAATGGTGACAGAGGGGCTTGGGGACAGCCTGCT
  GATGCTGGAGTTTCGGAAACCACCGTAGCTGAAAGTGAACCTCATATCCCATCTCTGTTATCTATGTCTA
  CAAGGAACCACATGGATATCACCATTCCACCCTTACCTCCAGTAGCTCCAGAAGTCTTGAGGGTTGCTGA
  ACACAGACACCGAAGGGGTCTTATGTATCCATATATTTACCACGTCCTTACTAAGGGTGAGATTAAAATC
  CCTGTATGTATTGAGGATGAATGTAACATGGAGCTGCCTCCAGCTGCTCTCCTGTTTCGGTCAGCTCGCC
  AATATGTATATGGAGTTCTTTTTAGCCTAGCAGAGACACAGCGAAAAATGGAGCGTCTGGCCATACGGCG
  CCGGTTACCTATGGAAGTTCCTTCTGTGATCCTTAAAGAGTGGTCTGCATATAAAGGGAAGTCACCTCAG
  ACCCCCGAGCTGGTATCTGCACTCACTTTTCGAGAATGGACTTGCCCAAACCTCAAGAAGCTCTGGTTAG
  GCAAAGCAGTTGAGGACAAGAACAGGAGGATGCGGGCTTTCCTGGCCTGTATGAAGTCAGACACGCCCAG
  TATGCTCAATCCAGCTAATGTCCCCACCCACCTGCTGCTCATGTGCTGTGTACTCCGATACATGGTTCAG
  TGGCCTGGTGGCCGAATCCTGCATCGCCATGAACTTGACACCTTCCTTGCACAGGCAGTTTCTACTCAGC
  TTTATGAACCAGATCAGCTTCAAGAACTCAAGATTGAGAAACTGGATGCTCGAGGGATCCAGCTTGCTGC
  CCTCTTCATGAGTGGGGTCGACACAGCTCTGTTTGCCAATGATGCATGTGGACAGCCAGTCCCATGGGAG
  CACTGCTGCCCCTGGATTTACTTTGATGGCAAGCTGTTCCAGAGCAAACTCATTAAAGCAGGCCGAGAGC
  GAGTATCACTGGTTGAACTCTGTGATGGCCAGGCTGACCTGGCCAGCAAAGTGGAGAAGATGCGGCAGAG
  CATCCTTGAAGGCGTCAACATGAATCATCCGCCACCTTCTGCTCTGCTCCCCTCACCTACTTTCGTGCCC
  CCCATGGTGCCCTCTCTCTACCCTGTTTCTCTGTATTCCAGAGCCATGGGCTCATTTCCACCTCCCCCTC
  AAGCAAGGAGTCGAGGGTTTGCAGGTCTCCATCCAATCCCACCCCAAGGAGGAAAACTGGAAATTGCTGG
  CATGGTGGTGGGCCAGTGGGCTGGCAGCAGATCTTCCAGAAGTCGAGGATCCTTTGGCATGCAAGTGGTT
  TCTGTCGGTGGGCCAGGAAAAGGACACGGAAAAGAACAGGCTGGCAGAGGATCCAAAGGTCACAAAAAAG
  GAAATAAACAAGGCTCTTCAGATGTAATTTCCAAAGCCGTGGAGCTTCATCAGAGTCGGGCCCGCTCACA
  GGTGAATGGAAACAATGGCACATTGATTGTGGAGGAGAAGAGCGATCCCCTTCCAGCTCCATCCCAGTGC
  GCCTTATCCAGAGACAGTAATGAGTGTAATAATAGCGATGACCACTGCCTCCCTGTGAAGAATGGAGAGA
  AGAACCATGTACCAGAGCAAGAGCTGGAAGCTGTGGCCCAACAGAAAGAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226693 representing NM_198105
  Red=Cloning site Green=Tags(s)
  
  MGVQGFQEFLEKRCPGAVVPVDLLKLARTVSRQQQQQHLHRQLPPAALAPGAPRITRGSAPLPPPPLPPA
  AFGAYSGGAGPSRHHHPAHHFHHHGQAPPGLHPPPPPPLPGARVLVDAGSALPRLYGGYQTDWVCGGQWN
  AMLGYLSALCQACAYPGGDGLELVVMFPGGLGKDRLAEWGRRCQAERQTAQLIVGHVGNKGTPPPRAWFL
  PPACLSHCVRLALIRFRVKVFQSLEDHHLEVVAFFRENGFHGLLAHDSEYALYNIPSYYSSHALKLSWNG
  KNLTTNQFLMQEVAKQLGLKRMNFPIFAALLGNHILPDEDLAAFHWSLLGPEHPLASLKVRAHQLVLPPC
  DVVIKAVSEYVSSIKDPSNLDVVGKDVFKQSQSRTEDKIERFKKAVEYYSVTTKLSSLPVGPSFLGFRNN
  RLGNPPLPRNQMGPISPGKPMFSRQVPQKMKYPPPFPMGPNSSLLFSHSVGESHAFSEDAMLQDNSFANW
  AVSYDSNTSQFPNCLTSKTSPPLGPDSSHSSSSDGDEANGAGSEQITEAVQQQPGWEDPNGDRGAWGQPA
  DAGVSETTVAESEPHIPSLLSMSTRNHMDITIPPLPPVAPEVLRVAEHRHRRGLMYPYIYHVLTKGEIKI
  PVCIEDECNMELPPAALLFRSARQYVYGVLFSLAETQRKMERLAIRRRLPMEVPSVILKEWSAYKGKSPQ
  TPELVSALTFREWTCPNLKKLWLGKAVEDKNRRMRAFLACMKSDTPSMLNPANVPTHLLLMCCVLRYMVQ
  WPGGRILHRHELDTFLAQAVSTQLYEPDQLQELKIEKLDARGIQLAALFMSGVDTALFANDACGQPVPWE
  HCCPWIYFDGKLFQSKLIKAGRERVSLVELCDGQADLASKVEKMRQSILEGVNMNHPPPSALLPSPTFVP
  PMVPSLYPVSLYSRAMGSFPPPPQARSRGFAGLHPIPPQGGKLEIAGMVVGQWAGSRSSRSRGSFGMQVV
  SVGGPGKGHGKEQAGRGSKGHKKGNKQGSSDVISKAVELHQSRARSQVNGNNGTLIVEEKSDPLPAPSQC
  ALSRDSNECNNSDDHCLPVKNGEKNHVPEQELEAVAQQKEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198105
• ORF Size: 3273 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198105.2, NP_932773.2
• RefSeq Size: 7709 bp
• RefSeq ORF: 3276 bp
• Locus ID: 207375
• UniProt ID: Q8C3F2
• Cytogenetics: X F3