Ids (NM_010498) Mouse Tagged ORF Clone

CAT#: MG226638

Ids (tGFP-tagged) - Mouse iduronate 2-sulfatase (Ids) transcript variant 1, (10ug)

Product Images

Circular map for MG226638

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ids
• Synonyms: AW214631
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226638 representing NM_010498
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCGCCGCCGCCGCCGCCTATCTGGCGGCAGCTTAGCTTCAGCTTGTTGCTAGGCTCCTTCTGCA
  TCGCCCTGGAGTCCGCAGCGCAAGGCAACTCTGCTACAGATGCTCTGAACATCCTTCTGATCATTGTGGA
  TGATCTCCGCCCCTCCCTGGGCTGTTATGGAGACAAGCTAGTGAGGTCCCCGAACATTGACCAGCTGGCA
  TCCCATAGCGTTCTCTTTCAGAATGCCTTTGCTCAGCAAGCAGTGTGCGCACCAAGTCGAGTGTCCTTCC
  TCACCGGGAGGAGGCCTGACACCACCCGCCTGTATGACTTCAATTCCTACTGGAGGGTACATTCTGGAAA
  TTTTTCCACCATCCCCCAATACTTCAAGGAGAATGGCTACGTGACCATGTCGGTTGGGAAAGTCTTTCAC
  CCTGGTATATCTTCCAATCACAGTGATGATTATCCATATAGCTGGTCTTTTCCACCTTATCATCCATCCT
  CAGAGAAATATGAAAACACTAAGACGTGTAAGGGACAAGATGGAAAACTCCACGCCAATCTGCTTTGTCC
  TGTCGATGTGGCAGATGTGCCTGAAGGTACCTTGCCTGACAAACAGAGCACAGAAGAAGCCATCAGGTTG
  TTGGAAAAGATGAAGACATCAGCTAGTCCTTTCTTCCTGGCAGTTGGGTACCACAAGCCGCATATCCCCT
  TCAGATACCCCAAGGAATTTCAGAAGTTGTATCCCTTGGAAAACATAACCCTGGCTCCTGATCCCCATGT
  TCCTGATAGCCTACCACCTGTAGCCTACAACCCCTGGATGGATATCAGGGAGAGGGAAGATGTCCAAGCC
  TTAAACATCAGTGTTCCCTATGGACCAATTCCTGAGGATTTTCAGCGGAAAATCCGCCAGAGCTACTTTG
  CTTCTGTGTCCTATTTGGATACTCAGGTTGGACATGTTTTGAGTGCTTTGGATGATCTTCGTCTAGCCCA
  CAACACAATTATTGCTTTCACATCTGATCACGGATGGGCCCTAGGTGAACACGGAGAATGGGCCAAGTAC
  AGCAACTTTGATGTTGCTACCCGTGTGCCCCTGATGCTCTATGTCCCTGGAAGGACGGCCCCACTTCCTG
  CGGCAGGCCAGAAGCTCTTTCCATACCGGGACCCCTTTGATCCGGCCTCGGATTGGATGGATGCAGGCAG
  GCACACCGAGGACCTCGTAGAACTTGTATCTCTCTTCCCCACGCTCGCTGGACTTGCAGGACTGCCAGTT
  CCTCCTCGGTGCCCCATCCCTTCTTTTCATGTTGAGCTTTGCAGAGAAGGCCAGAATCTTCAGAAGCATT
  TGCAGCTCCATGACTTGGAAGAGGAGCCAGACTTGTTTGGTAATCCCCGGGAGTTGATCGCCTATAGCCA
  GTACCCCCGGCCTGCAGATTTCCCTCAGTGGAATTCTGACAAGCCAAGCTTAAATGATATAAAGGTCATG
  GGATATTCTATACGCACTGTAGACTACAGGTACACTGTGTGGGTTGGCTTCGATCCTAGTGAATTTCTGG
  CTAACTTTTCTGATATCCATGCCGGGGAACTCTATTTTGTCGATTCTGACCCACTGCAGGATCACAATGT
  ATATAATGACTCCCAACATGGAGGTCTTCTCCACTCACTGAGGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226638 representing NM_010498
  Red=Cloning site Green=Tags(s)
  
  MSPPPPPPIWRQLSFSLLLGSFCIALESAAQGNSATDALNILLIIVDDLRPSLGCYGDKLVRSPNIDQLA
  SHSVLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHSGNFSTIPQYFKENGYVTMSVGKVFH
  PGISSNHSDDYPYSWSFPPYHPSSEKYENTKTCKGQDGKLHANLLCPVDVADVPEGTLPDKQSTEEAIRL
  LEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPHVPDSLPPVAYNPWMDIREREDVQA
  LNISVPYGPIPEDFQRKIRQSYFASVSYLDTQVGHVLSALDDLRLAHNTIIAFTSDHGWALGEHGEWAKY
  SNFDVATRVPLMLYVPGRTAPLPAAGQKLFPYRDPFDPASDWMDAGRHTEDLVELVSLFPTLAGLAGLPV
  PPRCPIPSFHVELCREGQNLQKHLQLHDLEEEPDLFGNPRELIAYSQYPRPADFPQWNSDKPSLNDIKVM
  GYSIRTVDYRYTVWVGFDPSEFLANFSDIHAGELYFVDSDPLQDHNVYNDSQHGGLLHSLRP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010498
• ORF Size: 1656 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010498.3, NP_034628.2
• RefSeq Size: 4988 bp
• RefSeq ORF: 1659 bp
• Locus ID: 15931
• UniProt ID: Q08890
• Cytogenetics: X 36.01 cM
• Gene Summary: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.[UniProtKB/Swiss-Prot Function]