Ddx58 (NM_172689) Mouse Tagged ORF Clone

CAT#: MG226477

Ddx58 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (Ddx58), (10ug)

Product Images

Circular map for MG226477

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ddx58
• Synonyms: 6430573D20Rik; C330021E21; RIG-I; RLR-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226477 representing NM_172689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCGGAGCAGCGGCAGAATCTGCAAGCATTCAGAGACTATATCAAGAAGATTCTGGACCCCACCT
  ACATCCTCAGCTACATGAGTTCCTGGCTCGAGGATGAGGAGGTGCAGTACATTCAGGCTGAGAAGAACAA
  CAAGGGCCCAATGGAAGCTGCCTCACTCTTCCTCCAGTACCTGTTGAAGCTGCAGTCAGAGGGCTGGTTC
  CAGGCCTTTTTGGATGCCCTGTACCATGCAGGTTACTGTGGACTTTGTGAAGCCATCGAAAGTTGGGACT
  TTCAAAAAATTGAAAAGTTAGAGGAACACAGATTACTTTTAAGACGTTTAGAACCAGAATTTAAGGCCAC
  AGTTGATCCAAATGATATCCTTTCTGAACTATCCGAATGTTTGATTAATCAGGAATGTGAAGAAATCAGA
  CAGATCCGAGACACTAAAGGGAGAATGGCAGGTGCGGAGAAGATGGCCGAATGTCTTATCAGATCCGACA
  AGGAAAACTGGCCAAAGGTCTTGCAACTTGCTTTGGAGAAAGACAACAGCAAGTTTAGTGAATTGTGGAT
  TGTTGATAAAGGTTTCAAAAGGGCTGAAAGCAAGGCTGATGAGGATGATGGAGCGGAGGCGTCCAGCATC
  CAGATTTTCATTCAGGAAGAGCCAGAGTGTCAGAATCTCAGTCAGAATCCCGGGCCTCCTTCAGAAGCGT
  CTTCTAATAATTTACACAGCCCATTGAAACCAAGAAATTACCAACTGGAGCTTGCCCTGCCTGCCAAGAA
  AGGGAAAAATACAATAATATGTGCCCCTACTGGTTGTGGAAAAACCTTTGTGTCGCTTCTTATATGTGAA
  CACCATCTTAAAAAATTCCCATGTGGACAAAAAGGGAAAGTGGTCTTCTTCGCTAACCAAATTCCTGTCT
  ATGAGCAGCAGGCAACTGTGTTCTCACGATATTTTGAAAGACTTGGGTACAACATTGCGAGCATTTCTGG
  GGCAACATCTGATAGCGTCTCAGTGCAGCACATCATTGAAGACAATGATATCATCATCCTGACACCCCAG
  ATTCTTGTGAACAATCTCAACAACGGAGCCATCCCCTCGTTGTCTGTCTTCACTCTGATGATATTTGATG
  AGTGTCATAACACTAGCAAAAACCACCCATACAATCAGATCATGTTCAGATACCTAGACCACAAACTTGG
  AGAGTCACGGGACCCACTGCCTCAGGTCGTTGGGCTGACTGCCTCCGTCGGCGTTGGAGATGCTAAGACC
  GCGGAGGAAGCCATGCAACATATCTGTAAACTCTGTGCCGCCCTGGATGCCTCCGTGATTGCCACAGTCA
  GAGACAACGTTGCAGAACTGGAACAGGTCGTTTATAAGCCCCAGAAAATTTCCAGGAAAGTGGCATCCCG
  GACTTCGAACACGTTTAAATGCATCATCTCTCAGCTGATGAAGGAGACAGAGAAGCTAGCCAAGGATGTC
  TCCGAGGAACTTGGAAAGCTTTTTCAAATTCAAAACAGAGAATTCGGCACCCAGAAATATGAACAGTGGA
  TTGTCGGCGTCCACAAAGCGTGCTCAGTGTTTCAGATGGCAGACAAAGAGGAGGAGAGCCGGGTCTGCAA
  AGCGCTCTTCCTGTACACATCACATTTGCGGAAATACAACGATGCACTCATCATCAGTGAGGATGCACAG
  ATGACAGACGCTCTAAATTACCTCAAAGCCTTCTTCCACGATGTCCGAGAAGCAGCATTCGATGAGACCG
  AGCGAGAGCTTACTCGGAGGTTTGAAGAAAAACTAGAGGAATTAGAAAAAGTTTCCAGGGATCCCAGCAA
  TGAGAATCCTAAACTAAGAGACCTCTACTTGGTCTTACAAGAAGAGTACCACTTAAAGCCAGAGACCAAG
  ACCATTCTCTTCGTGAAGACCAGAGCACTCGTGGATGCTCTGAAGAAATGGATTGAAGAAAATCCTGCAC
  TAAGCTTTCTAAAGCCTGGCATACTGACTGGGCGTGGCAGAACAAACCGGGCAACAGGAATGACGCTCCC
  GGCACAGAAGTGTGTGCTGGAGGCATTCAGAGCCAGCGGAGATAACAATATTCTGATTGCTACCTCGGTC
  GCTGATGAAGGCATTGACATTGCTGAGTGCAATCTCGTCATTCTCTATGAGTACGTGGGCAACGTCATCA
  AGATGATCCAAACCAGAGGCCGAGGAAGAGCACGAGATAGCAAGTGCTTCCTCCTGACCAGCAGCGCTGA
  CGTGATTGAAAAAGAAAAGGCGAACATGATCAAGGAAAAAATAATGAATGAATCCATCTTAAGACTGCAG
  ACATGGGATGAAATGAAATTTGGAAAGACGGTTCACCGCATACAGGTGAATGAAAAACTCCTCAGAGACA
  GTCAGCACAAACCACAACCTGTTCCTGACAAAGAAAACAAGAAACTGCTGTGTGGAAAGTGCAAGAATTT
  TGCGTGCTACACAGCTGACATTCGAGTGGTTGAGACGTCCCACTACACTGTCCTTGGAGACGCTTTTAAG
  GAGCGCTTTGTGTGTAAGCCACACCCTAAACCAAAGATCTATGACAATTTTGAGAAGAAAGCAAAGATAT
  TCTGCGCCAAACAGAACTGTAGCCACGACTGGGGAATTTTTGTGAGATACAAGACGTTCGAGATTCCAGT
  CATAAAAATTGAAAGTTTCGTCGTGGAAGATATTGTGAGCGGAGTTCAGAACCGGCACTCAAAGTGGAAG
  GACTTTCATTTTGAAAGGATACAGTTCGATCCTGCAGAAATGTCCGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226477 representing NM_172689
  Red=Cloning site Green=Tags(s)
  
  MTAEQRQNLQAFRDYIKKILDPTYILSYMSSWLEDEEVQYIQAEKNNKGPMEAASLFLQYLLKLQSEGWF
  QAFLDALYHAGYCGLCEAIESWDFQKIEKLEEHRLLLRRLEPEFKATVDPNDILSELSECLINQECEEIR
  QIRDTKGRMAGAEKMAECLIRSDKENWPKVLQLALEKDNSKFSELWIVDKGFKRAESKADEDDGAEASSI
  QIFIQEEPECQNLSQNPGPPSEASSNNLHSPLKPRNYQLELALPAKKGKNTIICAPTGCGKTFVSLLICE
  HHLKKFPCGQKGKVVFFANQIPVYEQQATVFSRYFERLGYNIASISGATSDSVSVQHIIEDNDIIILTPQ
  ILVNNLNNGAIPSLSVFTLMIFDECHNTSKNHPYNQIMFRYLDHKLGESRDPLPQVVGLTASVGVGDAKT
  AEEAMQHICKLCAALDASVIATVRDNVAELEQVVYKPQKISRKVASRTSNTFKCIISQLMKETEKLAKDV
  SEELGKLFQIQNREFGTQKYEQWIVGVHKACSVFQMADKEEESRVCKALFLYTSHLRKYNDALIISEDAQ
  MTDALNYLKAFFHDVREAAFDETERELTRRFEEKLEELEKVSRDPSNENPKLRDLYLVLQEEYHLKPETK
  TILFVKTRALVDALKKWIEENPALSFLKPGILTGRGRTNRATGMTLPAQKCVLEAFRASGDNNILIATSV
  ADEGIDIAECNLVILYEYVGNVIKMIQTRGRGRARDSKCFLLTSSADVIEKEKANMIKEKIMNESILRLQ
  TWDEMKFGKTVHRIQVNEKLLRDSQHKPQPVPDKENKKLLCGKCKNFACYTADIRVVETSHYTVLGDAFK
  ERFVCKPHPKPKIYDNFEKKAKIFCAKQNCSHDWGIFVRYKTFEIPVIKIESFVVEDIVSGVQNRHSKWK
  DFHFERIQFDPAEMSV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172689
• ORF Size: 2778 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172689.3, NP_766277.3
• RefSeq Size: 4943 bp
• RefSeq ORF: 2781 bp
• Locus ID: 230073
• UniProt ID: Q6Q899
• Cytogenetics: 4 A5
• Gene Summary: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: newcastle disease virus (NDV) and Sendai virus (SeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and orthoreovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.[UniProtKB/Swiss-Prot Function]