Usp19 (NM_001168372) Mouse Tagged ORF Clone

CAT#: MG226427

Usp19 (tGFP-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19) transcript variant 3, (10ug)

Product Images

Circular map for MG226427

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Usp19
• Synonyms: 8430421I07Rik; AI047774; Zmynd9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226427 representing NM_001168372
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCAGGGGCCAGTGCTACAGGGCCCAGGAGGGGGCCGCCAGGACTGGAAGAGGCCACTAGTAAGA
  AGAAACAGAAGGATCGAGCAAACCTGGAAAGTAAAGATGGAGATGCTAGGAGAGTGTCCCTTCCTCGAAA
  GGAACCAACCAAAGATGAATTGTTGCTCGATTGGAGGCAGAGTGCAGATGAGGTGATTGTTAAGCTGCGC
  GTGGGAACAGGTCCCGTACGTCTGGAGGATGTAGATGCTGCGTTCACAGACACGGACTGTGTGGTGAGGC
  TTCCAGATGGTCGGCAGTGGGGTGGTGTCTTCTTTGCTGAAATACAAAGTTCTTGCACCAAAGTGCAGGC
  TCGCAAGGGTGGTCTTCTACAGCTAGTACTACCCAAGAAGGTGCCTCTGCTCACGTGGCCCTCTCTCCTG
  AAACCTCTGGGAACCCAAGAGCTGGTGCCAGGTTTGCAGTGCCAGGAGAACGGGCAAGAGCTGTCTCCCA
  TTGCCCTGGAGCCAGGCTCTGAGCCCCGCAGAGCTAAACAGGAAGCCCGAAACCAGAAGCGGGCCCAGGG
  CCGTGGTGAGGTAGGCTCGGGGGCTGGCCCTGGGACACAGGCAGGGCCCAGCGCCAAGAGGGCTGTTCAC
  CTCCGCAGAGGGCCAGAAGGGGAAGGGTCCATGGATGGCCCCGGCCCCCAGGGTGATGCCCCGTCTTTCC
  TGTCTGACTCAGCTACCCAGGTTGAGGCTGAGGAGAAGCTCTGTGCTCCACCAATGAACACTCAAACAAG
  TCTCTTGAGCTCAGAGAAGAGTTTAGCCCTTCTGACAGTAGAGAAGACAGTGTCCCCCAGGAATGACCCA
  GTCGCCCCGGTTATGGTCCAGGACAGAGACCCTGAGCCTGAGCAAGAAGACCAAGTCAAAGAGGAGATGG
  CACTTGGGGCTGATCCTACAGCCTTGGTGGAGGAACCAGAGTCTATGGTGAACCTGGCATTTGTCAAGAA
  CGACTCGTATGAGAAGGGCCCGGATTCGGTGGTGGTGCACGTGTACGTGAAGGAGATCCGCAGGGACAGC
  TCCCGAGTGCTCTTCCGAGAGCAGGACTTCACACTGATCTTCCAGACCAGGGACGGAAACTTTCTGAGGC
  TGCATCCGGGCTGTGGGCCCCACACCATCTTCCGATGGCAGGTGAAGCTCAGAAACTTGATTGAACCAGA
  GCAGTGTACGTTCTGTTTCACGGCCTCTCGAATCGATATCTGCCTCCGGAAGCGGCAGAGTCAGCGCTGG
  GGGGGACTGGAGGCCCCTGCTACACGAGGTGCAGTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGCCCAA
  CCCCTTTGGATTCAACCCCTCCAGGAGGTGGCCCCCACCCCCTGACAGGCCAGGAGGAAGCCAGGGCTGT
  GGAGAAGGAAAAACCCAAGGCTCGATCAGAGGACTCAGGGCTGGATGGTGTGGTGGCCCGCACCCCCTTG
  GAGCATGTAGCCCCAAAGCCAGACCCACACTTGGCCTCGCCCAAACCCACGTGTATGGTGCCTCCAATGC
  CGCACAGTCCAGTTAGTGGGGATAGTGTGGAGGAGGACGAAGAGGAAGAGAAGAAGGTGTGCCTGCCAGG
  CTTCACTGGCCTTGTCAACTTAGGGAACACCTGCTTCATGAATAGCGTCATTCAGTCTTTGTCCAACACT
  CGGGAACTTCGTGACTTCTTTCACGACCGATCCTTTGAAGCTGAGATTAACTACAATAACCCATTGGGGA
  CTGGTGGGCGCCTCGCCATTGGCTTTGCTGTGCTGCTCCGGGCCCTATGGAAGGGTACTCACCAAGCCTT
  TCAGCCCTCCAAGCTAAAGGCCATTGTGGCAAGCAAGGCCAGCCAGTTCACAGGCTATGCACAGCATGAT
  GCTCAAGAGTTCATGGCTTTCTTGTTGGATGGGCTACATGAAGACCTCAATCGAATCCAAAACAAACCCT
  ACACAGAGACTGTGGACTCGGACGGGCGGCCCGATGAGGTGGTAGCCGAGGAAGCATGGCAGCGGCACAA
  GATGAGAAATGATTCATTCATTGTGGACCTGTTTCAGGGCCAGTACAAGTCAAAGCTGGTGTGCCCTGTG
  TGTGCCAAGGTCTCCATCACTTTTGACCCGTTCCTTTATCTGCCGGTACCCTTGCCACAAAAGCAAAAGG
  TTCTCCCCATATTTTATTTTGCCAGGGAGCCCCACAGCAAGCCCATCAAGTTCCTGGTGAGTGTCAGCAA
  GGAGAACTCCAGCGCGAGTGAAGTGTTGGACTCCCTCTCTCAGAGTGTCCACGTGAAGCCTGAGAACCTG
  CGCCTAGCCGAGGTAATTAAGAACCGTTTCCACCGTGTTTTCTTGCCCTCCCACTCACTGGACGCTGTGT
  CCCCCACGGACGTGCTCCTCTGCTTTGAGCTGCTCTCCCCAGAACTGGCTAAGGAGCGGGTAGTAGTGCT
  GGAGGTGCAGCAGCGCCCCCAGGTACCCAGCATCCCTATCTCCAAGTGCGCAGCCTGCCAGCGGAAGCAG
  CAATCAGAAGAAGAAAAGCTGAAGCGCTGTACCCGTTGCTACCGTGTGGGCTACTGCAACCAGTTCTGCC
  AGAAAACCCATTGGCCTGACCACAAAGGCCTCTGCCGCCCTGAGAACATTGGCTACCCCTTCCTGGTCAG
  TGTGCCTGCTTCACGCCTCACTTATGCCCGTCTTGCTCAGCTACTAGAAGGTTATGCCCGGTACTCTGTG
  AGTGTATTCCAACCGCCCTTCCAGCCTGGCCGCATGGCTTTGGAATCGCAGAGCCCTGGCTGTACCACGT
  TGCTTTCAACCAGCTCTCTGGAGGCTGGGGACAGTGAAAGAGAACCCATTCAGCCTTCTGAGCTCCAGCT
  GGTGACCCCTGTGGCTGAAGGGGATACAGGGGCTCACCGAGTATGGCCGCCTGCTGATAGGGGTCCTGTG
  CCTAGCACCAGTGGACTCTCTTCTGAGATGCTGGCCAGTGGGCCTATCGAAGGTTGTCCCTTGCTTGCTG
  GTGAGAGGGTATCTCGGCCTGAAGCTGCTGTGCCTGGGTACCAACACTCAAGTGAATCTGTGAATACCCA
  CACGCCCCAGTTCTTCATCTATAAAATTGATGCATCAAACCGTGAGCAGCGGCTGGAGGACAAAGGGGAG
  ACACCATTGGAGCTAGGTGATGACTGTAGCCTGGCTCTGGTGTGGCGGAACAATGAACGCCTGCAGGAGT
  TTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAAGATCCAGGCTCTGCTGGTGAGGCTGCCCGTGC
  TGGCCACTTCACCCTGGACCAGTGCCTCAACCTCTTTACACGGCCTGAAGTGCTGGCACCTGAGGAGGCC
  TGGTACTGCCCACAGTGCAAACAGCATCGTGAGGCCTCCAAACAGCTGCTGTTGTGGCGCCTACCGAACG
  TGCTGATTGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTCATTTGGCGAGACAAGATCAATGACTTGGT
  GGAGTTTCCTGTTCGGAACCTGGACTTGAGCAAGTTCTGTATCGGTCAGAAAGAGGAGCAGCTGCCTAGC
  TATGACCTGTATGCTGTCATCAACCACTACGGAGGCATGATCGGTGGCCACTATACTGCTTGTGCACGGC
  TGCCCAATGATCGCAGTAGCCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACGGTGACAAC
  AGTAGACGAAAGCCAGGTCGTGACGCGCTATGCCTATGTTCTCTTCTACCGTCGTCGGAACTCTCCTGTG
  GAGAGACCCCCCAGGGCAAGTCACTCTGAACACCACCCAGACCTAGGCCCTGCAGCTGAGGCTGCTGCCA
  GCCAGGGACTAGGCCCTGGCCAGGCCCCCGAGGTGGCCCCCACGCGGACAGCCCCTGAACGCTTCGCCCC
  CCCTGTGGACCGCCCAGCCCCCACGTACAGCAACATGGAGGAGGTCGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226427 representing NM_001168372
  Red=Cloning site Green=Tags(s)
  
  MSAGASATGPRRGPPGLEEATSKKKQKDRANLESKDGDARRVSLPRKEPTKDELLLDWRQSADEVIVKLR
  VGTGPVRLEDVDAAFTDTDCVVRLPDGRQWGGVFFAEIQSSCTKVQARKGGLLQLVLPKKVPLLTWPSLL
  KPLGTQELVPGLQCQENGQELSPIALEPGSEPRRAKQEARNQKRAQGRGEVGSGAGPGTQAGPSAKRAVH
  LRRGPEGEGSMDGPGPQGDAPSFLSDSATQVEAEEKLCAPPMNTQTSLLSSEKSLALLTVEKTVSPRNDP
  VAPVMVQDRDPEPEQEDQVKEEMALGADPTALVEEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEIRRDS
  SRVLFREQDFTLIFQTRDGNFLRLHPGCGPHTIFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRW
  GGLEAPATRGAVGGAKVAVPTGPTPLDSTPPGGGPHPLTGQEEARAVEKEKPKARSEDSGLDGVVARTPL
  EHVAPKPDPHLASPKPTCMVPPMPHSPVSGDSVEEDEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSNT
  RELRDFFHDRSFEAEINYNNPLGTGGRLAIGFAVLLRALWKGTHQAFQPSKLKAIVASKASQFTGYAQHD
  AQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPV
  CAKVSITFDPFLYLPVPLPQKQKVLPIFYFAREPHSKPIKFLVSVSKENSSASEVLDSLSQSVHVKPENL
  RLAEVIKNRFHRVFLPSHSLDAVSPTDVLLCFELLSPELAKERVVVLEVQQRPQVPSIPISKCAACQRKQ
  QSEEEKLKRCTRCYRVGYCNQFCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYSV
  SVFQPPFQPGRMALESQSPGCTTLLSTSSLEAGDSEREPIQPSELQLVTPVAEGDTGAHRVWPPADRGPV
  PSTSGLSSEMLASGPIEGCPLLAGERVSRPEAAVPGYQHSSESVNTHTPQFFIYKIDASNREQRLEDKGE
  TPLELGDDCSLALVWRNNERLQEFVLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEEA
  WYCPQCKQHREASKQLLLWRLPNVLIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPS
  YDLYAVINHYGGMIGGHYTACARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSPV
  ERPPRASHSEHHPDLGPAAEAAASQGLGPGQAPEVAPTRTAPERFAPPVDRPAPTYSNMEEVD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168372
• ORF Size: 3969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168372.2, NP_001161844.1
• RefSeq Size: 4725 bp
• RefSeq ORF: 3972 bp
• Locus ID: 71472
• UniProt ID: Q3UJD6
• Cytogenetics: 9 F2
• Gene Summary: Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates (By similarity). Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing thier ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Exhibits a preference towards 'Lys-63'-linked ubiquitin chains (By similarity). Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis.[UniProtKB/Swiss-Prot Function]