Ntrk3 (NM_182809) Mouse Tagged ORF Clone

CAT#: MG226356

Ntrk3 (tGFP-tagged) - Mouse neurotrophic tyrosine kinase receptor type 3 (Ntrk3) transcript variant 2, (10ug)

Product Images

Circular map for MG226356

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ntrk3
• Synonyms: AW125844; Ntrk3_tv3; TrkC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226356 representing NM_182809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTCTCTCTTTGCCCAGCCAAGTGTAGTTTCTGGCGGATTTTCTTGCTGGGAAGCGTCTGGCTGG
  ACTATGTGGGCTCCGTGCTGGCTTGCCCTGCAAATTGTGTCTGCAGCAAGACTGAGATCAATTGCCGGCG
  GCCGGACGATGGGAACCTCTTCCCTCTCCTGGAAGGGCAGGATTCAGGGAACAGCAATGGGAACGCCAGC
  ATCAACATTACGGACATCTCAAGGAATATCACTTCCATACACATAGAGAACTGGCGAGGCCTGCACACAC
  TCAATGCTGTGGACATGGAGCTCTACACGGGACTCCAGAAGCTGACCATCAAGAATTCTGGACTCCGGAA
  CATCCAGCCCAGAGCCTTCGCCAAGAACCCCCACTTGCGTTATATAAACTTGTCAAGTAACCGGCTCACC
  ACACTCTCCTGGCAGCTCTTCCAGACGCTGAGCCTTCGGGAATTGAGACTGGAGCAGAACTTCTTCAACT
  GCAGCTGTGACATCCGCTGGATGCAGCTGTGGCAGGAACAGGGGGAGGCGCGGCTGGACAGCCAGAGCCT
  TTACTGCATCAGTGCTGATGGCTCCCAACTCCCCCTCTTCCGCATGAACATCAGTCAGTGTGATCTCCCA
  GAGATCAGTGTGAGCCACGTCAACCTGACTGTCCGAGAAGGAGACAATGCCGTGATCACTTGCAATGGCT
  CTGGCTCTCCTTTGCCTGATGTGGACTGGATAGTCACTGGGCTGCAGTCCATCAACACCCACCAGACCAA
  TCTGAACTGGACCAATGTACATGCCATCAACTTGACCCTGGTGAACGTGACGAGCGAGGACAATGGCTTC
  ACCCTGACGTGCATTGCAGAGAACGTGGTGGGCATGAGCAATGCCAGTGTTGCTCTCACTGTCTACTACC
  CTCCACGTGTGGTGAGCCTGGTGGAGCCTGAGGTACGCCTGGAACATTGCATTGAGTTTGTGGTGCGTGG
  CAACCCGACACCCACGCTCCACTGGCTGTACAATGGACAGCCATTGAGGGAGTCCAAGATCATTCACATG
  GACTACTACCAGGAGGGGGAGGTCTCAGAGGGCTGCCTGCTCTTCAACAAGCCCACCCACTACAACAATG
  GCAACTACACCCTCATTGCTAAGAATGCCCTGGGCACGGCCAACCAGACCATCAACGGCCACTTCCTGAA
  GGAGCCCTTTCCAGAGAGCACAGATTTCTTTGACTTTGAGTCTGATGCGAGCCCTACACCTCCTATCACT
  GTGACCCACAAACCAGAGGAAGACACTTTTGGGGTGTCCATAGCAGTCGGACTTGCTGCCTTTGCCTGCG
  TCCTTCTGGTGGTTCTCTTTATCATGATCAACAAGTATGGTCGCCGGTCCAAATTTGGAATGAAGGGTCC
  TGTGGCTGTTATCAGTGGAGAGGAGGACTCAGCCAGCCCACTGCATCACATCAACCATGGCATCACTACA
  CCATCATCGTTGGATGCTGGGCCTGACACAGTGGTCATTGGCATGACCCGCATCCCAGTCATTGAGAACC
  CCCAGTACTTCCGTCAGGGTCACAATTGCCACAAGCCAGACACATGGGTCTTTTCAAACATAGACAATCA
  TGGGATATTAAACTTGAAGGACAATAGAGATCATCTAGTCCCATCAACTCACTATATATATGAGGAACCT
  GAGGTCCAGAGTGGGGATGTGTCTTACCCAAGGTCACATGGTTTCAGAGAAATTATGTTGAATCCAATAA
  GCCTTTCCGGACATTCCAAGCCTCTTAACCATGGCATCTATGTTGAGGATGTCAATGTTTATTTCAGCAA
  AGGACGTCATGGCTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226356 representing NM_182809
  Red=Cloning site Green=Tags(s)
  
  MDVSLCPAKCSFWRIFLLGSVWLDYVGSVLACPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNAS
  INITDISRNITSIHIENWRGLHTLNAVDMELYTGLQKLTIKNSGLRNIQPRAFAKNPHLRYINLSSNRLT
  TLSWQLFQTLSLRELRLEQNFFNCSCDIRWMQLWQEQGEARLDSQSLYCISADGSQLPLFRMNISQCDLP
  EISVSHVNLTVREGDNAVITCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNGF
  TLTCIAENVVGMSNASVALTVYYPPRVVSLVEPEVRLEHCIEFVVRGNPTPTLHWLYNGQPLRESKIIHM
  DYYQEGEVSEGCLLFNKPTHYNNGNYTLIAKNALGTANQTINGHFLKEPFPESTDFFDFESDASPTPPIT
  VTHKPEEDTFGVSIAVGLAAFACVLLVVLFIMINKYGRRSKFGMKGPVAVISGEEDSASPLHHINHGITT
  PSSLDAGPDTVVIGMTRIPVIENPQYFRQGHNCHKPDTWVFSNIDNHGILNLKDNRDHLVPSTHYIYEEP
  EVQSGDVSYPRSHGFREIMLNPISLSGHSKPLNHGIYVEDVNVYFSKGRHGF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182809
• ORF Size: 1836 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182809.2, NP_877961.1
• RefSeq Size: 3847 bp
• RefSeq ORF: 1839 bp
• Locus ID: 18213
• UniProt ID: Q6VNS1
• Cytogenetics: 7 44.01 cM
• Gene Summary: Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.[UniProtKB/Swiss-Prot Function]