Kcnk18 (NM_207261) Mouse Tagged ORF Clone

CAT#: MG226304

Kcnk18 (tGFP-tagged) - Mouse potassium channel subfamily K member 18 (Kcnk18), (10ug)

Product Images

Circular map for MG226304

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcnk18
• Synonyms: Gm781; Tresk; Tresk-2; Trik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226304 representing NM_207261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTGAGGAGCCACCTGAGGCCAGGAGATGCTGTCCCGAGGCCCTGGGGAAGGCCAGGGGATGCT
  GCCCCGAAGCCCTGGGCAAGCTTCTGCCCGGCCTCTGCTTCCTTTGCTGCCTGGTGACCTATGCGCTGGT
  GGGTGCTGCTCTCTTCTCCGCTGTCGAGGGCCGCCCTGACCCAGAAGCAGAGGAGAACCCTGAGTTGAAG
  AAGTTCCTGGACGATCTGTGCAACATCCTGAAATGTAACCTGACAGTGGTTGAAGGTAGCAGGAAGAACT
  TGTGTGAGCATCTGCAACACCTCAAGCCCCAGTGGCTCAAGGCGCCCCAGGACTGGTCCTTCCTGAGCGC
  TCTCTTCTTCTGCTGCACAGTGTTCAGCACAGTGGGTTATGGCCACATGTACCCTGTCACCAGGCTCGGT
  AAGTTCCTGTGCATGCTGTATGCGCTCTTTGGAATCCCTCTAATGTTCCTGGTCCTCACAGACATAGGAG
  ATATCCTGGCCACCATCTTATCCAGGGCTTACAGTCGGTTCCAGGCTCTCCTTTGCCTCCCCCACGATAT
  CTTCAAATGGCGCTCCCTCCCGCTCTGCCGGAAGCAGCCTGACAGCAAACCGGTGGAGGAAGCCATCCCT
  CAGATTGTCATTGATGCTGGTGTGGATGAACTCCTAAACCCGCAGCCCAGCAAGGACCCCCCCTCTCCGA
  GCTGCAATGTGGAGCTGTTTGAGAGATTAGTTGCCCGTGAGAAAAAGAACAAGCTACAACCACCCACGCG
  TCCCGTGGAGAGGAGCAACTCCTGTCCCGAGCTGGTGCTGGGGCGACTGTCCTGTTCTATTCTCAGCAAT
  CTGGATGAAGTGGGCCAGCAGGTGGAGAGGCTGGACATCCCTCTCCCCGTCATCGCCCTGGTCGTCTTTG
  CATACATCTCCTGCGCGGCTGCTATCCTCCCCTTCTGGGAGACCGAGCTAGGCTTCGAGGATGCTTTCTA
  CTTCTGCTTTGTGACACTGACCACCATCGGGTTTGGGGACATCGTGCTGGTGCACCCTCATTTCTTCCTC
  TTCTTCTCCATCTACATCATCGTGGGCATGGAGATCCTGTTCATTGCCTTCAAGCTGATGCAGAACCGGC
  TCCTGCACACCTACAAAACCCTCATGCTGTTTGTTTGCCAAAGGGAAGTTTCGCTACCTTGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226304 representing NM_207261
  Red=Cloning site Green=Tags(s)
  
  MEAEEPPEARRCCPEALGKARGCCPEALGKLLPGLCFLCCLVTYALVGAALFSAVEGRPDPEAEENPELK
  KFLDDLCNILKCNLTVVEGSRKNLCEHLQHLKPQWLKAPQDWSFLSALFFCCTVFSTVGYGHMYPVTRLG
  KFLCMLYALFGIPLMFLVLTDIGDILATILSRAYSRFQALLCLPHDIFKWRSLPLCRKQPDSKPVEEAIP
  QIVIDAGVDELLNPQPSKDPPSPSCNVELFERLVAREKKNKLQPPTRPVERSNSCPELVLGRLSCSILSN
  LDEVGQQVERLDIPLPVIALVVFAYISCAAAILPFWETELGFEDAFYFCFVTLTTIGFGDIVLVHPHFFL
  FFSIYIIVGMEILFIAFKLMQNRLLHTYKTLMLFVCQREVSLPW
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207261
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207261.3, NP_997144.1
• RefSeq Size: 3032 bp
• RefSeq ORF: 1185 bp
• Locus ID: 332396
• UniProt ID: Q6VV64
• Cytogenetics: 19 D3
• Gene Summary: Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation.[UniProtKB/Swiss-Prot Function]