Tnfsf10 (NM_009425) Mouse Tagged ORF Clone

CAT#: MG226180

Tnfsf10 (tGFP-tagged) - Mouse tumor necrosis factor (ligand) superfamily member 10 (Tnfsf10), (10ug)

Product Images

Circular map for MG226180

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tnfsf10
• Synonyms: A330042I21Rik; AI448571; APO-2L; Ly81; TL2; Tnlg6a; Trail
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226180 representing NM_009425
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCCTCAGGGGCCCTGAAGGACCTCAGCTTCAGTCAGCACTTCAGGATGATGGTGATTTGCATAG
  TGCTCCTGCAGGTGCTCCTGCAGGCTGTGTCTGTGGCTGTGACTTACATGTACTTCACCAACGAGATGAA
  GCAGCTGCAGGACAATTACTCCAAAATTGGACTAGCTTGCTTCTCAAAGACGGATGAGGATTTCTGGGAC
  TCCACTGATGGAGAGATCTTGAACAGACCCTGCTTGCAGGTTAAGAGGCAACTGTATCAGCTCATTGAAG
  AGGTGACTTTGAGAACCTTTCAGGACACCATTTCTACAGTTCCAGAAAAGCAGCTAAGTACTCCTCCCTT
  GCCCAGAGGTGGAAGACCTCAGAAAGTGGCAGCTCACATTACTGGGATCACTCGGAGAAGCAACTCAGCT
  TTAATTCCAATCTCCAAGGATGGAAAGACCTTAGGCCAGAAGATTGAATCCTGGGAGTCCTCTCGGAAAG
  GGCATTCATTTCTCAACCACGTGCTCTTTAGGAATGGAGAGCTGGTCATCGAGCAGGAGGGCCTGTATTA
  CATCTATTCCCAAACATACTTCCGATTTCAGGAAGCTGAAGACGCTTCCAAGATGGTCTCAAAGGACAAG
  GTGAGAACCAAACAGCTGGTGCAGTACATCTACAAGTACACCAGCTATCCGGATCCCATAGTGCTCATGA
  AGAGCGCCAGAAACAGCTGTTGGTCCAGAGATGCCGAGTACGGACTGTACTCCATCTATCAGGGAGGATT
  GTTCGAGCTAAAAAAAAATGACAGGATTTTTGTTTCTGTGACAAATGAACATTTGATGGACCTGGATCAA
  GAAGCCAGCTTCTTTGGAGCCTTTTTAATTAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226180 representing NM_009425
  Red=Cloning site Green=Tags(s)
  
  MPSSGALKDLSFSQHFRMMVICIVLLQVLLQAVSVAVTYMYFTNEMKQLQDNYSKIGLACFSKTDEDFWD
  STDGEILNRPCLQVKRQLYQLIEEVTLRTFQDTISTVPEKQLSTPPLPRGGRPQKVAAHITGITRRSNSA
  LIPISKDGKTLGQKIESWESSRKGHSFLNHVLFRNGELVIEQEGLYYIYSQTYFRFQEAEDASKMVSKDK
  VRTKQLVQYIYKYTSYPDPIVLMKSARNSCWSRDAEYGLYSIYQGGLFELKKNDRIFVSVTNEHLMDLDQ
  EASFFGAFLIN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009425
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009425.2, NP_033451.1
• RefSeq Size: 4944 bp
• RefSeq ORF: 876 bp
• Locus ID: 22035
• UniProt ID: P50592
• Cytogenetics: 3 A3
• Gene Summary: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.[UniProtKB/Swiss-Prot Function]