Tnfsf10 (NM_009425) Mouse Untagged Clone

CAT#: MC209584

Tnfsf10 (untagged) - Mouse tumor necrosis factor (ligand) superfamily, member 10 (Tnfsf10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnfsf10
• Synonyms: A330042I21Rik; AI448571; APO-2L; Ly81; TL2; Tnlg6a; Trail
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209584 representing NM_009425
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCCTCAGGGGCCCTGAAGGACCTCAGCTTCAGTCAGCACTTCAGGATGATGGTGATTTGCATAG
  TGCTCCTGCAGGTGCTCCTGCAGGCTGTGTCTGTGGCTGTGACTTACATGTACTTCACCAACGAGATGAA
  GCAGCTGCAGGACAATTACTCCAAAATTGGACTAGCTTGCTTCTCAAAGACGGATGAGGATTTCTGGGAC
  TCCACTGATGGAGAGATCTTGAACAGACCCTGCTTGCAGGTTAAGAGGCAACTGTATCAGCTCATTGAAG
  AGGTGACTTTGAGAACCTTTCAGGACACCATTTCTACAGTTCCAGAAAAGCAGCTAAGTACTCCTCCCTT
  GCCCAGAGGTGGAAGACCTCAGAAAGTGGCAGCTCACATTACTGGGATCACTCGGAGAAGCAACTCAGCT
  TTAATTCCAATCTCCAAGGATGGAAAGACCTTAGGCCAGAAGATTGAATCCTGGGAGTCCTCTCGGAAAG
  GGCATTCATTTCTCAACCACGTGCTCTTTAGGAATGGAGAGCTGGTCATCGAGCAGGAGGGCCTGTATTA
  CATCTATTCCCAAACATACTTCCGATTTCAGGAAGCTGAAGACGCTTCCAAGATGGTCTCAAAGGACAAG
  GTGAGAACCAAACAGCTGGTGCAGTACATCTACAAGTACACCAGCTATCCGGATCCCATAGTGCTCATGA
  AGAGCGCCAGAAACAGCTGTTGGTCCAGAGATGCCGAGTACGGACTGTACTCCATCTATCAGGGAGGATT
  GTTCGAGCTAAAAAAAAATGACAGGATTTTTGTTTCTGTGACAAATGAACATTTGATGGACCTGGATCAA
  GAAGCCAGCTTCTTTGGAGCCTTTTTAATTAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009425
• Insert Size: 876 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009425.2, NP_033451.1
• RefSeq Size: 4944 bp
• RefSeq ORF: 876 bp
• Locus ID: 22035
• UniProt ID: P50592
• Cytogenetics: 3 A3
• Gene Summary: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.[UniProtKB/Swiss-Prot Function]