Ephb1 (NM_001168296) Mouse Tagged ORF Clone

CAT#: MG226017

Ephb1 (tGFP-tagged) - Mouse Eph receptor B1 (Ephb1) transcript variant 2, (10ug)

Product Images

Circular map for MG226017

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ephb1
• Synonyms: 9330129L11; AW488255; C130099E04Rik; Cek6; Elk; Elkh; ENSMUSG00000074119; Hek6; Net
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226017 representing NM_001168296
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGATTGCTTGCTGCTCTTCCTCCTGGCATCTGCAGTGGCCGCGATGGAAGAGACATTGATGG
  ACACAAGGACAGCCACTGCAGAGTTGGGATGGACGGCCAACCCTGCCTCTGGGTGGGAAGAAGTCAGTGG
  CTATGATGAAAACCTGAACACCATCCGTACTTACCAAGTGTGCAACGTCTTCGAACCCAACCAGAACAAC
  TGGCTGCTTACCACCTTTATCAACAGAAGGGGCGCCCATCGCATCTATACAGAGATGCGCTTCACTGTGA
  GGGACTGCAGCAGCCTTCCAAATGTCCCAGGCTCCTGCAAGGAGACCTTCAACTTGTACTACTATGAGAC
  TGACTCTGTGATTGCCACCAAGAAGTCAGCCTTCTGGTCTGAAGCCCCCTACCTCAAAGTGGACACCATT
  GCTGCAGATGAGAGCTTCTCCCAGGTGGATTTTGGGGGAAGGTTGATGAAAGTCAACACGGAAGTCAGGA
  GCTTTGGGCCTCTTACTAGGAACGGTTTTTACCTCGCTTTCCAGGATTATGGAGCCTGTATGTCTCTCCT
  TTCTGTCCGTGTCTTCTTCAAAAAGTGTCCCAGCATCGTGCAGAATTTTGCAGTGTTCCCAGAAACCATG
  ACAGGAGCAGAGAGCACATCTCTGGTGATTGCTCGGGGCACATGCATCCCAAATGCGGAAGAAGTGGATG
  TGCCCATAAAACTCTACTGCAACGGAGATGGAGAGTGGATGGTGCCCATTGGGCGCTGTACCTGTAAGCC
  TGGCTATGAGCCTGAGAACAGCGTGGCCTGCAAGGCCTGTCCTGCGGGGACCTTCAAGGCCAGCCAGGAA
  GCTGAAGGCTGCTCCCACTGCCCCTCCAACAGTCGCTCCCCTTCAGAGGCGTCTCCCATCTGCACCTGCC
  GGACTGGCTATTACCGAGCTGACTTTGATCCACCAGAGGTGGCGTGTACTAGTGTCCCATCGGGTCCTCG
  AAATGTCATCTCCATCGTGAATGAGACATCTATCATTCTAGAGTGGCACCCTCCAAGAGAGACTGGTGGG
  AGAGATGACGTGACGTACAACATCATCTGCAAGAAGTGCCGAGCAGACCGCCGCAGCTGCTCCCGCTGCG
  ATGACAATGTGGAGTTTGTCCCCAGGCAACTGGGCTTGACTGAGTGTCGTGTCTCCATCAGTAGCCTATG
  GGCCCACACCCCGTACACCTTTGATATCCAGGCCATCAATGGAGTCTCTAGCAAGAGTCCCTTTCCCCCA
  CAGCACGTCTCTGTCAACATCACCACAAACCAAGCTGCCCCCTCCACTGTTCCTATCATGCACCAGGTCA
  GTGCCACCATGAGGAGCATCACCTTGTCATGGCCTCAGCCGGAGCAACCCAATGGCATAATCCTGGACTA
  TGAGATCCGGTACTATGAGAAGGAACACAATGAGTTCAACTCTTCCATGGCCAGGAGCCAGACCAACACA
  GCACGTATCGATGGGCTACGGCCTGGCATGGTATACGTGGTCCAGGTGCGAGCTCGAACCGTGGCTGGCT
  ATGGCAAGTTCAGTGGCAAGATGTGTTTCCAGACTCTGACAGATGATGATTACAAGTCGGAGCTGAGAGA
  GCAGCTACCCCTGATTGCTGGCTCGGCAGCAGCTGGAGTCGTATTTGTTGTGTCTCTGGTGGCCATCTCT
  ATTGTCTGCAGCAGGAAACGAGCTTACAGCAAAGAGGCTGCGTACAGTGATAAGCTTCAACATTACAGCA
  CAGGCCGAGGGGAGTTCGGCGAGGTGTACAAGGGCCGTTTGAAGCTGCCAGGCAAGAGGGAAATCTATGT
  GGCCATCAAGACCCTGAAGGCTGGGTACTCAGAGAAACAGCGTCGGGATTTTCTGAGCGAGGCGAGCATC
  ATGGGCCAGTTTGACCATCCCAACATCATTCGCCTGGAGGGTGTCGTCACCAAGAGCCGGCCTGTCATGA
  TCATTACGGAGTTCATGGAGAACGGCGCTTTAGACTCTTTCCTCCGGCAAAATGATGGACAGTTCACCGT
  GATCCAGCTTGTGGGGATGCTGAGGGGCATCGCTGCTGGCATGAAGTACCTATCTGAGATGAATTATGTG
  CACCGGGACCTGGCTGCTAGAAACATTCTGGTCAACAGCAACCTGGTGTGCAAAGTTTCTGACTTTGGTC
  TCTCTCGCTACCTCCAGGATGACACCTCAGACCCCACCTACACCAGCTCCTTGGGAGGGAAGATCCCTGT
  GAGATGGACAGCTCCAGAGGCCATCGCCTACCGCAAGTTTACGTCAGCCAGCGATGTCTGGAGCTATGGG
  ATTGTCATGTGGGAAGTGATGTCATTTGGAGAGAGACCTTACTGGGATATGTCCAATCAAGATGTCATCA
  ATGCCATTGAGCAGGATTACCGGCTGCCTCCTCCTATGGACTGCCCAGCTGCCCTGCACCAGCTCATGCT
  GGACTGTTGGCAGAAGGATCGCAATAGCCGGCCCCGTTTTGCAGAGATCGTCAACACCCTGGACAAGATG
  ATCCGGAACCCAGCTAGTCTCAAGACTGTGGCAACCATCACCGCTGTGCCTTCCCAACCCCTGCTTGACC
  GCTCTATCCCAGACTTCACGGCCTTTACCACCGTGGATGACTGGCTAAGTGCCATCAAAATGGTCCAGTA
  CAGGGACAGCTTCCTCACCGCAGGCTTCACCTCCCTTCAGCTGGTCACCCAGATGACATCAGAAGACCTC
  CTGAGAATAGGGGTAACCTTGGCAGGCCATCAGAAGAAGATTCTGAGTAGCATTCACTCAATGAGGGTCC
  AGATGAACCAGTCACCATCGGTAATGGCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226017 representing NM_001168296
  Red=Cloning site Green=Tags(s)
  
  MALDCLLLFLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNN
  WLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTI
  AADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETM
  TGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQE
  AEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGG
  RDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPP
  QHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNT
  ARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAIS
  IVCSRKRAYSKEAAYSDKLQHYSTGRGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASI
  MGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLSEMNYV
  HRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYG
  IVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKM
  IRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDL
  LRIGVTLAGHQKKILSSIHSMRVQMNQSPSVMA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168296
• ORF Size: 2829 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168296.1, NP_001161768.1
• RefSeq Size: 4563 bp
• RefSeq ORF: 2832 bp
• Locus ID: 270190
• UniProt ID: Q8CBF3
• Cytogenetics: 9 F1
• Gene Summary: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (PubMed:27446912).[UniProtKB/Swiss-Prot Function]