Mau2 (NM_001167939) Mouse Tagged ORF Clone

CAT#: MG225966

Mau2 (tGFP-tagged) - Mouse RIKEN cDNA 9130404D08 gene (9130404D08Rik) transcript variant 1, (10ug)

Product Images

Circular map for MG225966

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mau2
• Synonyms: 9130404D08Rik; A930019L04Rik; C77863; C79014; Mau-2; mKIAA0892
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225966 representing NM_001167939
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCACAGGCGGCGGCTGCGGCGCAGGCGGCGGCTGCAGCTCAGGCTGCCGCCGCACAGGCCGCAC
  AGGCCGAGGCAGCCGAGTCGTGGTATCTGGCTCTCCTGGGTTTCGCCGAGCACTTCCGAACATCTAGCCC
  GCCCAAGATCCGCTTGTGCGTTCATTGCCTGCAGGCCGTGTTCCCCTTCAAGCCGCCTCAGCGGATCGAG
  GCCCGGACGCACCTGCAGCTCGGCTCGGTGCTTTACCACCACACGAAGAACAGCGAGCAGGCGCGCAGCC
  ACCTGGAGAAGGCGTGGTTGATATCACAGCAAATCCCACAGTTTGAAGATGTTAAATTTGAGGCAGCAAG
  CCTGTTGTCAGAGCTGTATTGTCAAGAGAACTCCGTGGATGCAGCAAAACCACTGCTGCGGAAAGCCATC
  CAGATCTCACAACAGACGCCATACTGGCACTGTCGCCTGCTCTTCCAGCTCGCTCAACTGCACACACTGG
  AGAAGGACCTGGTATCCGCCTGTGACCTCCTAGGCGTGGGGGCTGAGTATGCCCGAGTGGTGGGATCTGA
  ATATACAAGGGCATTATTCCTGCTCAGCAAGGGGATGCTGCTGTTGATGGAGCGCAAACTGCAAGAGGTG
  CACCCACTGCTCACACTGTGTGGGCAGATTGTAGAGAACTGGCAGGGCAACCCCATCCAGAAGGAGTCAC
  TACGTGTCTTCTTCCTGGTGCTGCAGGTGACCCACTACCTGGATGCTGGGCAGGTGAAGAGCGTCAAGCC
  GTGCCTGAAGCAGCTGCAGCAGTGCATTCAGACCATCTCCACACTACATGATGATGAAATCCTGCCCAGC
  AACCCTGCTGACCTCTTCCACTGGCTGCCCAAGGAGCACATGTGTGTGCTCGTCTACCTGGTCACAGTGA
  TGCATTCGATGCAGGCTGGTTACCTGGAGAAGGCACAGAAGTACACAGACAAGGCGCTCATGCAGTTGGA
  GAAGCTTAAGATGCTGGACTGCAGCCCTATCCTGTCGTCTTTCCAAGTGATCCTGCTGGAGCATATCATC
  ATGTGCCGGCTTGTCACAGGCCACAAGGCCACTGCACTACAGGAGATCTCCCAGGTCTGCCAGCTATGCC
  AGCAGTCCCCTAGGCTCTTCTCCAACCATGCTGCCCAGTTGCACACACTGTTGGGCCTGTATTGTGTCTC
  CGTGAACTGCATGGATAATGCTGAAGCCCAATTTACCACAGCCCTTCGGCTCACCAACCACCAGGAGTTG
  TGGGCCTTCATCGTGACCAACCTTGCGAGTGTGTATATACGAGAAGGAAATAGACACCAAGAGGTACTGT
  ACAGTTTGCTTGAGAGGATAAACCCGGACCACAGCTTCCCGGTCAGCTCACACTGCCTCCGAGCAGCAGC
  CTTCTACGTGCGAGGGCTCTTCTCCTTCTTCCAGGGCCGCTACAACGAAGCCAAGCGGTTTCTTCGAGAA
  ACTCTGAAGATGTCAAATGCAGAGGACCTGAACCGCCTCACAGCCTGCTCCCTTGTGCTGCTGGGTCACA
  TCTTCTATGTGTTGGGAAATCACAGGGAGAGTAACAACATGGTGGTACCTGCCATGCAGCTGGCCAGCAA
  GATCCCAGACATGTCTGTGCAGCTGTGGTCATCTGCCCTCCTGAGAGACCTAAACAAGGCATGTGGGAAC
  GCCATGGATGCCCATGAAGCCGCACAGATGCACCAGAACTTCTCACAGCAGCTGCTGCAGGACCACATCG
  AGGCCTGTAGCCTCCCAGAGCACAACCTCATTACGATGGCCCACCCCCTGTGCAGTTCCAAGCTCAGAAT
  GGACCCAACACCAGCCTGGCCAGCCTTCTGTGAGGCCTGTGTCCGCAACTTGGACCCAACACTGGAGCTC
  TCTGGGAACAAAACCACCAGGGTGCCCACCAGTCCCTCACCATTGTCCAGAGCTAAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225966 representing NM_001167939
  Red=Cloning site Green=Tags(s)
  
  MAAQAAAAAQAAAAAQAAAAQAAQAEAAESWYLALLGFAEHFRTSSPPKIRLCVHCLQAVFPFKPPQRIE
  ARTHLQLGSVLYHHTKNSEQARSHLEKAWLISQQIPQFEDVKFEAASLLSELYCQENSVDAAKPLLRKAI
  QISQQTPYWHCRLLFQLAQLHTLEKDLVSACDLLGVGAEYARVVGSEYTRALFLLSKGMLLLMERKLQEV
  HPLLTLCGQIVENWQGNPIQKESLRVFFLVLQVTHYLDAGQVKSVKPCLKQLQQCIQTISTLHDDEILPS
  NPADLFHWLPKEHMCVLVYLVTVMHSMQAGYLEKAQKYTDKALMQLEKLKMLDCSPILSSFQVILLEHII
  MCRLVTGHKATALQEISQVCQLCQQSPRLFSNHAAQLHTLLGLYCVSVNCMDNAEAQFTTALRLTNHQEL
  WAFIVTNLASVYIREGNRHQEVLYSLLERINPDHSFPVSSHCLRAAAFYVRGLFSFFQGRYNEAKRFLRE
  TLKMSNAEDLNRLTACSLVLLGHIFYVLGNHRESNNMVVPAMQLASKIPDMSVQLWSSALLRDLNKACGN
  AMDAHEAAQMHQNFSQQLLQDHIEACSLPEHNLITMAHPLCSSKLRMDPTPAWPAFCEACVRNLDPTLEL
  SGNKTTRVPTSPSPLSRAKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167939
• ORF Size: 1950 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167939.2
• RefSeq Size: 5365 bp
• RefSeq ORF: 1953 bp
• Locus ID: 74549
• UniProt ID: Q9D2X5
• Cytogenetics: 8 B3.3
• Gene Summary: Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodim. eric complex (also known as cohesin loading complex) with NIPBL/SCC2 which mediates the loading of the cohesin complex onto chromatin Plays a role in sister chromatid cohesion and normal progression through prometaphase.[UniProtKB/Swiss-Prot Function]