Tcf3 (NM_001164149) Mouse Tagged ORF Clone

CAT#: MG225780

Tcf3 (tGFP-tagged) - Mouse transcription factor 3 (Tcf3) transcript variant 3, (10ug)

Product Images

Circular map for MG225780

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tcf3
• Synonyms: A1; AA408400; ALF2; AW209082; bHLHb21; E2A; E12; E12/E47; E47; KA1; ME2; Pan1; Pan2; TCF-3; Tcfe2a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225780 representing NM_001164149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAACCAGTCTCAGAGAATGGCACCCGTGGGCTCTGACAAGGAACTGAGTGACCTCCTGGACTTCA
  GCATGATGTTCCCGCTACCTGTGGCCAATGGGAAGAGCCGGCCCGCCTCCCTCGGGGGAACCCAGTTTGC
  AGGCTCAGGACTGGAGGACCGACCCAGCTCAGGCTCCTGGGGCAGCAGTGACCAGAACAGTTCTTCCTTT
  GACCCTAGCCGGACATACAGCGAAGGTGCCCACTTCAGTGACTCCCACAGCAGCCTGCCGCCTTCCACGT
  TCCTAGGAGCTGGGCTTGGAGGCAAGGGCAGTGAGCGGAATGCCTATGCCACCTTTGGGAGAGACACCAG
  TGTTGGCACCTTGAGTCAGGCTGGCTTCCTGCCAGGTGAGCTGAGCCTCAGCAGTCCCGGGCCACTGTCC
  CCATCGGGCATCAAGAGCAGCTCCCAGTATTACCCCTCATTCCCCAGCAACCCTCGTCGGAGAGCTGCAG
  ATGGTGGCCTGGCAGATACTCAGCCGAAGAAGGTCCGGAAGGTTCCGCCTGGTCTCCCTTCCTCGGTGTA
  TCCGCCCAGCTCAGGTGACAGCTACAGCAGGGATGCTGCAGCCTACCCCTCCGCCAAGACCCCCAGCAGC
  GCTTACCCCTCCCCCTTCTACGTGGCAGATGGCAGCCTGCACCCATCAGCTGAGCTCTGGAGTACGCCTA
  GCCAGGTGGGCTTTGGGCCCATGCTAGGTGACGGCTCTTCCCCTCTGCCCCTTGCACCGGGCAGCAGCTC
  CGTGGGCAGTGGTACCTTTGGGGGCCTCCAGCAGCAGGATCGCATGGGCTACCAGCTGCATGGATCTGAG
  GTTAATGGCTCGCTCCCAGCTGTATCCAGCTTTTCGGCTGCCCCTGGCACTTACAGTGGGACTTCCGGCC
  ACACGCCCCCTGTGAGTGGGGCCGCAGCTGAAAGCCTCCTAGGCACCCGAGGGACTACAGCCAGCAGCTC
  AGGGGATGCCCTTGGGAAGGCACTGGCCTCGATCTACTCCCCGGATCACTCCAGCAATAATTTCTCACCT
  AGCCCCTCAACGCCTGTGGGTTCACCCCAGGGCCTGCCAGGGACATCACAGTGGCCCCGGGCAGGAGCGC
  CCAGTGCCTTATCCCCCAACTACGATGCAGGTCTCCATGGCCTGAGCAAGATGGAGGACCGCTTGGACGA
  GGCCATCCATGTCCTGCGAAGCCACGCTGTTGGCACCGCTAGCGATCTCCATGGGCTTTTGCCTGGCCAT
  GGCGCACTGACCACGAGCTTCACCGGCCCCATGTCACTGGGCGGGCGGCATGCCGGCCTGGTCGGGGGAA
  GCCATCCTGAGGAGGGCCTCACAAGTGGGGCCAGTCTTTTGCATAACCATGCCAGCCTCCCCAGCCAGCC
  CAGTTCCCTCCCTGACCTCTCACAGAGACCTCCCGACTCCTATAGTGGACTCGGGAGGGCAGGCACAACA
  GCGGGTGCCAGCGAGATCAAGCGGGAGGAGAAAGAGGATGAGGAAATCGCATCAGTAGCCGACGCCGAAG
  AGGACAAGAAGGACCTGAAGGTCCCACGCACGCGCACCAGCCCAGACGAGGACGAGGACGACCTTCTCCC
  CCCAGAGCAGAAGGCGGAGCGGGAGAAGGAGCGCCGGGTGGCCAATAATGCCCGAGAGCGCCTGCGGGTC
  CGCGACATCAATGAGGCCTTTAAGGAGCTCGGCCGCATGTGCCAGCTGCACCTCAGCAGCGAGAAGCCGC
  AGACCAAACTGCTCATCCTGCACCAGGCCGTGGCCGTCATCCTCAGCCTGGAGCAGCAGGTGCGAGAACG
  CAACCTGAACCCCAAAGCAGCCTGCTTGAAGCGGAGGGAGGAGGAGAAGGTGTCTGGCGTGGTCGGGGAC
  CCACAGCTGGCCCTGTCAGCCGCCCACCCGGGCCTGGGTGAGGCCCACAACCCAGCCGGGCACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225780 representing NM_001164149
  Red=Cloning site Green=Tags(s)
  
  MMNQSQRMAPVGSDKELSDLLDFSMMFPLPVANGKSRPASLGGTQFAGSGLEDRPSSGSWGSSDQNSSSF
  DPSRTYSEGAHFSDSHSSLPPSTFLGAGLGGKGSERNAYATFGRDTSVGTLSQAGFLPGELSLSSPGPLS
  PSGIKSSSQYYPSFPSNPRRRAADGGLADTQPKKVRKVPPGLPSSVYPPSSGDSYSRDAAAYPSAKTPSS
  AYPSPFYVADGSLHPSAELWSTPSQVGFGPMLGDGSSPLPLAPGSSSVGSGTFGGLQQQDRMGYQLHGSE
  VNGSLPAVSSFSAAPGTYSGTSGHTPPVSGAAAESLLGTRGTTASSSGDALGKALASIYSPDHSSNNFSP
  SPSTPVGSPQGLPGTSQWPRAGAPSALSPNYDAGLHGLSKMEDRLDEAIHVLRSHAVGTASDLHGLLPGH
  GALTTSFTGPMSLGGRHAGLVGGSHPEEGLTSGASLLHNHASLPSQPSSLPDLSQRPPDSYSGLGRAGTT
  AGASEIKREEKEDEEIASVADAEEDKKDLKVPRTRTSPDEDEDDLLPPEQKAEREKERRVANNARERLRV
  RDINEAFKELGRMCQLHLSSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSGVVGD
  PQLALSAAHPGLGEAHNPAGHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164149
• ORF Size: 1953 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164149.1, NP_001157621.1
• RefSeq Size: 3314 bp
• RefSeq ORF: 1956 bp
• Locus ID: 21423
• UniProt ID: P15806
• Cytogenetics: 10 39.72 cM
• Gene Summary: Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.[UniProtKB/Swiss-Prot Function]