Tpm3 (NM_022314) Mouse Tagged ORF Clone

CAT#: MG225688

Tpm3 (tGFP-tagged) - Mouse tropomyosin 3 gamma (Tpm3), (10ug)

Product Images

Circular map for MG225688

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tpm3
• Synonyms: gamma-TM; hTM30nm; hTMnm; Tm5NM; TM30nm; TMnm; Tpm-5; Tpm5; Trop-5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225688 representing NM_022314
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCATCAAGAAAAAGATGCAGATGCTGAAGTTAGACAAAGAGAATGTTCTGGACCGAGCTGAGC
  AAGCTGAAGCTGAGCAGAAACAGGCAGAAGAAAGAAGCAAGCAGCTAGAGGATGAACTAGCAACCATGCA
  GAAGAAGCTGAAAGGGACAGAGGATGAGCTGGACAAGTATTCGGAAGCTTTAAAGGATGCTCAGGAGAAG
  CTGGAGCTAGCAGAGAAGAAGGCAGCCGACGCTGAAGCTGAGGTGGCCTCCTTGAACCGCAGGATCCAGC
  TGGTTGAAGAGGAGCTGGACCGTGCGCAGGAGCGCCTTGCCACTGCTTTGCAGAAGCTGGAGGAAGCAGA
  GAAGGCTGCTGATGAGAGTGAGAGAGGTATGAAGGTGATTGAAAATCGGGCTCTAAAAGATGAAGAAAAG
  ATGGAACTCCAGGAAATCCAGCTAAAGGAAGCAAAGCACATTGCAGAAGAGGCCGATAGGAAGTATGAAG
  AGGTGGCTCGTAAGTTGGTGATTATTGAAGGAGACTTGGAACGCACGGAGGAACGTGCTGAGCTGGCAGA
  GTCCCGTTGCCGAGAGATGGATGAACAGATCAGACTGATGGACCAGAACCTGAAGTGTCTGAGTGCTGCT
  GAAGAAAAGTACTCTCAAAAAGAAGACAAGTATGAAGAAGAAATAAAGATTCTTACTGATAAACTCAAGG
  AGGCAGAGACCCGTGCTGAGTTTGCTGAAAGATCGGTAGCCAAGCTGGAGAAGACCATTGATGACCTGGA
  AGATAAACTGAAGTGCACCAAAGAGGAGCATCTCTGTACACAAAGGATGCTGGACCAGACCCTGCTGGAC
  CTGAACGAGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225688 representing NM_022314
  Red=Cloning site Green=Tags(s)
  
  MEAIKKKMQMLKLDKENVLDRAEQAEAEQKQAEERSKQLEDELATMQKKLKGTEDELDKYSEALKDAQEK
  LELAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEK
  MELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAA
  EEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDKLKCTKEEHLCTQRMLDQTLLD
  LNEM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022314
• ORF Size: 855 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022314.2
• RefSeq Size: 2239 bp
• RefSeq ORF: 858 bp
• Locus ID: 59069
• Cytogenetics: 3 F1
• Gene Summary: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.[UniProtKB/Swiss-Prot Function]