Prom1 (NM_001163583) Mouse Tagged ORF Clone

CAT#: MG225624

Prom1 (tGFP-tagged) - Mouse prominin 1 (Prom1) transcript variant 6, (10ug)

Product Images

Circular map for MG225624

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prom1
• Synonyms: 4932416E19Rik; AC133; CD133; Prom; Prom-1; Proml1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225624 representing NM_001163583
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCGTCTTCAGTGCCCTGCTGTTACTGGGGCTGTGTGGAAAGATCTCTTCAGAAGGTCAGCCTG
  CATTCCATAACACTCCTGGGGCTATGAATTATGAATTGCCTACCACCAAATATGAGACCCAAGATACCTT
  CAATGCTGGGATTGTTGGCCCTCTCTACAAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGAC
  TTCCCTCTAGATTTGATCAAAAAACTCATACAGAACAAGAACTTTGACATCTCAGTTGATTCCAAGGAGA
  TTGCCCTCTATGAGATCGGAGTCCTTATCTGCGCCATCCTGGGACTGCTGTTCATTATCCTCATGCCTCT
  GGTGGGCTGCTTCTTTTGTATGTGCCGTTGCTGCAACAAATGCGGCGGAGAGATGCACCAGCGGCAGAAG
  CAGAATGCGCCATGCAGGAGGAAGTGCTTGGGCCTCTCCCTCCTGGTGATTTGTCTGCTCATGAGCCTTG
  GCATTATATATGGCTTTGTGGCTAACCAGCAGACCAGGACTCGGATCAAAGGGACCCAGAAACTGGCAAA
  GAGCAATTTCAGAGACTTTCAAACACTCCTGACTGAAACACCAAAGCAAATTGACTATGTAGTGGAGCAG
  TACACCAACACCAAGAACAAGGCATTCTCAGACCTGGATGGCATCGGCTCCGTGCTGGGAGGCAGAATAA
  AGGACCAACTAAAACCCAAAGTAACTCCTGTCCTCGAAGAGATTAAGGCCATGGCGACAGCCATCAAACA
  GACCAAGGATGCCCTGCAGAACATGAGCAGCAGCCTGAAAAGTCTCCAAGATGCAGCCACCCAGCTCAAT
  ACCAACCTGAGCTCTGTGAGAAACAGCATCGAGAATTCGCTCAGCAGCAGTGACTGTACCTCAGATCCAG
  CCAGCAAGATCTGCGATAGCATCAGACCAAGCCTAAGCAGTCTGGGGAGCAGCCTCAATTCAAGTCAGCT
  CCCATCAGTGGATAGAGAACTCAACACTGTTACTGAAGTCGACAAAACTGATCTGGAGAGCCTCGTCAAA
  AGGGGGTATACGACAATTGATGAAATACCCAATACAATACAAAACCAAACTGTGGATGTCATCAAAGACG
  TCAAAAATACCTTGGACTCCATTAGCTCCAACATTAAGGACATGAGCCAAAGTATTCCTATTGAGGATAT
  GCTGTTACAGGTCTCCCATTACCTTAATAACAGCAACAGATACTTAAACCAGGAGCTGCCCAAGCTGGAA
  GAATATGACTCGTACTGGTGGCTGGGTGGCTTGATTGTCTGCTTTCTGCTGACTCTCATTGTGACCTTCT
  TTTTCCTGGGCTTGCTGTGTGGTGTGTTTGGCTATGACAAGCATGCCACCCCAACTAGAAGAGGCTGTGT
  GTCCAACACTGGAGGCATCTTCCTCATGGCTGGGGTTGGATTCGGCTTCCTTTTTTGCTGGATATTGATG
  ATCCTTGTGGTTCTTACGTTTGTTGTTGGTGCAAATGTGGAAAAGTTGCTCTGCGAACCTTATGAAAACA
  AGAAATTATTACAGGTTTTGGACACTCCCTATCTGCTCAAGGAACAATGGCAATTTTATCTTTCTGGCAT
  GCTATTCAATAACCCAGACATTAACATGACCTTTGAGCAAGTCTACAGGGATTGCAAAAGAGGTCGAGGT
  ATATATGCTGCTTTTCAGCTTGAGAATGTCGTCAACGTCAGTGATCATTTCAACATTGACCAGATTTCTG
  AAAACATAAATACGGAGTTGGAAAACCTGAATGTGAACATTGATAGCATTGAACTGTTGGATAACACAGG
  AAGGAAGAGCCTCGAGGACTTTGCACATTCTGGGATAGATACAATCGATTATTCCACATACTTGAAGGAG
  ACTGAGAAATCCCCTACTGAAGTGAATCTGCTGACATTTGCCTCTACCCTGGAAGCAAAAGCAAACCAGT
  TGCCTGAAGGAAAGCTGAAACAGGCCTTCTTACTGGATGTACAGAATATAAGAGCCATCCACCAGCATCT
  CCTCCCTCCTGTGCAGCAATCACTGAATACGTTAAGACAAAGTGTCTGGACCCTCCAGCAAACAAGCAAC
  AAGTTGCCGGAGAAAGTGAAGAAGATCCTTGCCTCTTTGGACTCTGTTCAGCATTTCCTCACCAATAACG
  TTTCCCTCATCGTTATCGGGGAAACGAAGAAGTTTGGGAAAACAATACTAGGCTACTTTGAACATTATCT
  GCACTGGGTCTTTTATGCCATCACAGAGAAGATGACATCCTGCAAACCCATGGCCACCGCGATGGACTCT
  GCTGTTAATGGCATTCTGTGTGGCTATGTTGCGGACCCTCTGAATTTGTTCTGGTTCGGCATAGGGAAAG
  CCACGGTGCTCTTACTTCCGGCTGTAATCATTGCTATCAAGCTGGCCAAGTACTATCGCAGGATGGATTC
  AGAGGATGTATACGACGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225624 representing NM_001163583
  Red=Cloning site Green=Tags(s)
  
  MALVFSALLLLGLCGKISSEGQPAFHNTPGAMNYELPTTKYETQDTFNAGIVGPLYKMVHIFLNVVQPND
  FPLDLIKKLIQNKNFDISVDSKEIALYEIGVLICAILGLLFIILMPLVGCFFCMCRCCNKCGGEMHQRQK
  QNAPCRRKCLGLSLLVICLLMSLGIIYGFVANQQTRTRIKGTQKLAKSNFRDFQTLLTETPKQIDYVVEQ
  YTNTKNKAFSDLDGIGSVLGGRIKDQLKPKVTPVLEEIKAMATAIKQTKDALQNMSSSLKSLQDAATQLN
  TNLSSVRNSIENSLSSSDCTSDPASKICDSIRPSLSSLGSSLNSSQLPSVDRELNTVTEVDKTDLESLVK
  RGYTTIDEIPNTIQNQTVDVIKDVKNTLDSISSNIKDMSQSIPIEDMLLQVSHYLNNSNRYLNQELPKLE
  EYDSYWWLGGLIVCFLLTLIVTFFFLGLLCGVFGYDKHATPTRRGCVSNTGGIFLMAGVGFGFLFCWILM
  ILVVLTFVVGANVEKLLCEPYENKKLLQVLDTPYLLKEQWQFYLSGMLFNNPDINMTFEQVYRDCKRGRG
  IYAAFQLENVVNVSDHFNIDQISENINTELENLNVNIDSIELLDNTGRKSLEDFAHSGIDTIDYSTYLKE
  TEKSPTEVNLLTFASTLEAKANQLPEGKLKQAFLLDVQNIRAIHQHLLPPVQQSLNTLRQSVWTLQQTSN
  KLPEKVKKILASLDSVQHFLTNNVSLIVIGETKKFGKTILGYFEHYLHWVFYAITEKMTSCKPMATAMDS
  AVNGILCGYVADPLNLFWFGIGKATVLLLPAVIIAIKLAKYYRRMDSEDVYDE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163583
• ORF Size: 2469 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163583.1, NP_001157055.1
• RefSeq Size: 2965 bp
• RefSeq ORF: 2472 bp
• Locus ID: 19126
• UniProt ID: O54990
• Cytogenetics: 5 B3
• Gene Summary: May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.[UniProtKB/Swiss-Prot Function]