Cadps2 (NM_153163) Mouse Tagged ORF Clone

CAT#: MG225617

Cadps2 (tGFP-tagged) - Mouse Ca2+-dependent activator protein for secretion 2 (Cadps2), (10ug)

Product Images

Circular map for MG225617

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cadps2
• Synonyms: A230044C21Rik; Caps-2; Caps2; Cpd2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225617 representing NM_153163
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGACCCGTCTTCCAGCGAGGAGGAGTCGGACGAGGGGCTGGAGGAGGAGAGCCGCGAGGTGTTGG
  TGGCGCCGGGCGTCTCCCAGCGAGCGCCGCCGGCCGCGGCTCGGGAAGGGCGGCGGGACGCGCCGGGACG
  CTCCGGTGGTGGCAGCGGCGGCGGAGCGGCCAGACCCGTGAGCCCGAGCCCCTCGGTACTCAGCGAGGGG
  CGAAATGAGCCCGAGCTGCAGCTGGACGAGGAGCAGGAGCGGCGCATCCGCCTGCAGCTCTACGTCTTCG
  TGGTGAGGTGCATCGCGTACCCATTCAACGCCAAGCAGCCCACCGACATGGCCCGGAGGCAGCAGAAGCT
  TAACAAACAACAATTGCAGTTACTGAAAGAACGGTTCCAGGCCTTTCTCAATGGAGAAACTCAGATAGTA
  GCAGACGAAGCCTTCTGCAATGCCGTCCGGAGTTACTATGAGGTGTTTCTAAAGAGTGACCGTGTGGCCA
  GAATGGTTCAAAGTGGAGGATGCTCTGCTAATGATTTCAGAGAGGTGTTTAAGAAGAACATAGAAAAACG
  TGTCCGTAGCCTGCCAGAGATAGATGGCCTGAGCAAAGAGACAGTGCTGAGCTCATGGATAGCCAAATAT
  GACGCCATTTACAGGGGAGAAGAAGATTTGTGCAAACAGCCGAACAGGATGACCCTCAGTGCCGTGTCTG
  AGCTTATTCTGAGTAAGGAGCAGCTCTATGAAATGTTCCAGCAGATTCTGGGCATTAAGAAGCTGGAACA
  CCAGCTACTTTATAACGCATGCCAGCTGGATAACGCTGATGAACAAGCAGCCCAGATCAGAAGGGAACTT
  GATGGCCGCCTGCAGTTGGCAGAGAAGATGGCAAAGGAGAGAAGATTCCCCAGATTCATCTCGAAAGAAA
  TGGAGAGTATGTACATTGAAGAGCTGCGAGCCTCCGTGAACCTGCTAATGGCCAATTTAGAAAGTCTTCC
  AGTTTCAAAAGGTGGCCCGGAATTTAAATTACAAAAATTAAAGCGTTCGCAGAACTCTGCGTTTCTGGAC
  CTGGGAGATGAGAACGAGATCCAGCTGTCCAAGTCGGATGTGGTGTTGTCGTTCACGTTAGAGATTGTCA
  TCATGGAAGTGCAAGGACTGAAATCTGTGGCTCCCAATCGAATCGTTTACTGCACAATGGAGGTGGAAGG
  AGGAGAAAAACTCCAGACAGACCAGGCTGAAGCATCAAGGCCACAATGGGGGACCCAAGGAGATTTCAAC
  ACTACCCACCCTCGGCCTGTCGTCAAAGTGAAGCTCTTCACAGAAAGCACGGGGGTCCTGGCCCTGGAAG
  ACAAGGAACTGGGCAGGGTGGTGTTATACCCAACTTCTAATAGCTCCAAGTCAGCAGAGTTACACCGAAT
  GACAGTACCCAAGAACAGTCAGGACTCGGACCTAAAGATCAAATTGGCAGTGCGCATGGATAAACCAGCA
  CACATGAAGCATAGTGGGTACCTGTATGCCCTTGGACAGAAGGTTTGGAAAAGATGGAAAAAGCGTTACT
  TTGTTCTCGTTCAGGTTAGCCAGTACACCTTTGCTATGTGCAGCTATAGAGAAAAAAAGTCGGAACCACA
  GGAATTAATGCAACTGGAAGGATACACAGTGGATTACACAGACCCTCACCCAGGCCTTCAGGGTGGTCAG
  GTGTTCTTCAACGCTGTTAAAGAGGGAGATACTGTGATCTTTGCCAGTGATGATGAACAGGACAGAATAT
  TATGGGTACAAGCCATGTACAGGGCTACAGGCCAGTCTTACAAACCAGTTCCTGCAGTCCAAAGCCAGAA
  GCTGAATCCTAAAGGCGGAGCTCTCCATGCAGATGCTCAGCTTTATGCAGACCGTTTTCAGAAACACGGG
  ATGGATGAGTTTATTTCTGCGAGTCCTTGCAAGCTTGACCATGCCTTCCTCTTCAGAATTCTCCAGAGAC
  AGACTTTGGATCACAGACTGAATGATTCGTATTCTTGTTTGGGGTGGTTTAGCCCTGGCCAAGTCTTTGT
  GTTAGATGAGTACTGTGCCCGCTACGGAGTGAGAGGCTGTCACAGGCATCTCTGCTACCTTACAGAACTG
  ATGGAACATTCAGAAAACGGTGCTGTCATTGACCCCACCCTGCTCCATTACAGCTTTGCATTCTGTGCCT
  CTCACGTGCACGGCAACAGGCCTGATGGGATTGGAACGGTTTCAGTGGAAGAGAAAGAAAGATTTGAGGA
  GATAAAAGACCGACTTTCTTCGCTTTTAGAAAACCAGATCAGCCACTTCAGATACTGCTTTCCCTTCGGA
  CGACCTGAGGGTGCCCTAAAAGCTACGCTCTCCTTACTTGAAAGGGTTTTAATGAAAGACATTGCCACTC
  CTATCCCTGCGGAGGAGGTGAAGAAAGTGGTCAGAAAATGTCTGGAGAAAGCTGCCTTGATCAATTACAC
  TAGGCTCACAGAATATGCCAAAATAGAAGGGCCCGCAGAAAAGGAAACAGAGACCATGAACCAGGCAACT
  CCTGCCAGGAAGCTGGAAGAGGTTCTTCATCTTGCAGAGCTCTGCATAGAAGTCCTACAGCAAAATGAGG
  AGCATCATGCTGAGGGAAGAGAGGCATTTGCCTGGTGGCCTGACTTGTTGGCCGAGCATGCAGAGAAGTT
  TTGGGCTTTATTCACAGTAGACATGGATACTGCGCTGGAGGCCCAACCTCAAGACTCCTGGGATAGCTTT
  CCCCTTTTCCAGCTGCTTAATAATTTCCTCAGAAATGACACACTTTTGTGTAATGGAAAATTCCACAAGC
  ACTTGCAAGAAATCTTTGTGCCCTTGGTTGTCCGCTACGTTGACCTGATGGAGTCTGCCATCGCCCAGTC
  CATTCACAGAGGTTTTGAGCAGGAGACATGGCAGCCTGTCAAGAATATCGCCAACAGTCTTCCCAATGTA
  GCTCTTCCAAAAGTTCCAAGTCTGCCTCTTAATCTTCCACAGATTCCTAGCTTTTCTACTCCTCCGTGGA
  TGGCTTCTTTATATGAGTCCACCAATGGCTCAACAACTTCCGAGGATCTGTTCTGGAAGCTCGATGCGCT
  GCAAATGTTCGTCTTTGATCTCCATTGGCCAGAACAAGAGTTTGCCCACCACTTAGAGCAAAGACTTAAA
  CTAATGGCCAGTGATATGATAGAGGCGTGTGTCAAAAGAACAAGAACTGCGTTCGAACTCAAGCTACAAA
  AGGCAAACAAAACAACTGACTTGCGCATCCCAGCTTCCGTGTGCACAATGTTTAATGTATTAGTTGATGC
  TAAAAAGCAAAGCACCAAGCTGTGTGCCCTGGATGGAGGACAAGAGCAACAGTACCATTCAAAAATAGAT
  GATTTGATTGACAACACCGTGAAAGAAATCATTGCACTGCTGGTTTCAAAGTTTGTTTCAGTGTTGGAAG
  GGGTGCTTTCGAAGTTGTCGAGGTATGACGAAGGCACTTTCTTCTCATCCATCCTGTCCTTCACTGTGAA
  AGCAGCTGCAAAATATGTGGATGTCCCTAAACCAGGAATGGATCTGGCAGACACCTACATTATGTTTGTC
  CGGCAAAACCAGGATATTCTTCGAGAAAAAGTCAATGAAGAGATGTACATAGAAAAGTTGTTTGATCAAT
  GGTACAGCAATTCCATGAAAGTCATTTGTGTGTGGCTGGCTGACAGACTAGACCTCCAGCTTCATATTTA
  CCAACTGAAGACGCTCATCAAGATTGTGAAGAAAACCTATAGGGATTTCCGATTGCAGGGTGTGTTGGAG
  GGGACGCTGAACAGTAAGACATATGATACTCTGCACAGACGTCTAACTGTAGAGGAGGCCACAGCCTCTG
  TCTCAGAAGGCGGAGGACTTCAGGGCATTACCATGAAGGACAGTGATGAGGAGGAAGAAGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225617 representing NM_153163
  Red=Cloning site Green=Tags(s)
  
  MLDPSSSEEESDEGLEEESREVLVAPGVSQRAPPAAAREGRRDAPGRSGGGSGGGAARPVSPSPSVLSEG
  RNEPELQLDEEQERRIRLQLYVFVVRCIAYPFNAKQPTDMARRQQKLNKQQLQLLKERFQAFLNGETQIV
  ADEAFCNAVRSYYEVFLKSDRVARMVQSGGCSANDFREVFKKNIEKRVRSLPEIDGLSKETVLSSWIAKY
  DAIYRGEEDLCKQPNRMTLSAVSELILSKEQLYEMFQQILGIKKLEHQLLYNACQLDNADEQAAQIRREL
  DGRLQLAEKMAKERRFPRFISKEMESMYIEELRASVNLLMANLESLPVSKGGPEFKLQKLKRSQNSAFLD
  LGDENEIQLSKSDVVLSFTLEIVIMEVQGLKSVAPNRIVYCTMEVEGGEKLQTDQAEASRPQWGTQGDFN
  TTHPRPVVKVKLFTESTGVLALEDKELGRVVLYPTSNSSKSAELHRMTVPKNSQDSDLKIKLAVRMDKPA
  HMKHSGYLYALGQKVWKRWKKRYFVLVQVSQYTFAMCSYREKKSEPQELMQLEGYTVDYTDPHPGLQGGQ
  VFFNAVKEGDTVIFASDDEQDRILWVQAMYRATGQSYKPVPAVQSQKLNPKGGALHADAQLYADRFQKHG
  MDEFISASPCKLDHAFLFRILQRQTLDHRLNDSYSCLGWFSPGQVFVLDEYCARYGVRGCHRHLCYLTEL
  MEHSENGAVIDPTLLHYSFAFCASHVHGNRPDGIGTVSVEEKERFEEIKDRLSSLLENQISHFRYCFPFG
  RPEGALKATLSLLERVLMKDIATPIPAEEVKKVVRKCLEKAALINYTRLTEYAKIEGPAEKETETMNQAT
  PARKLEEVLHLAELCIEVLQQNEEHHAEGREAFAWWPDLLAEHAEKFWALFTVDMDTALEAQPQDSWDSF
  PLFQLLNNFLRNDTLLCNGKFHKHLQEIFVPLVVRYVDLMESAIAQSIHRGFEQETWQPVKNIANSLPNV
  ALPKVPSLPLNLPQIPSFSTPPWMASLYESTNGSTTSEDLFWKLDALQMFVFDLHWPEQEFAHHLEQRLK
  LMASDMIEACVKRTRTAFELKLQKANKTTDLRIPASVCTMFNVLVDAKKQSTKLCALDGGQEQQYHSKID
  DLIDNTVKEIIALLVSKFVSVLEGVLSKLSRYDEGTFFSSILSFTVKAAAKYVDVPKPGMDLADTYIMFV
  RQNQDILREKVNEEMYIEKLFDQWYSNSMKVICVWLADRLDLQLHIYQLKTLIKIVKKTYRDFRLQGVLE
  GTLNSKTYDTLHRRLTVEEATASVSEGGGLQGITMKDSDEEEEG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153163
• ORF Size: 3912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153163.4, NP_694803.3
• RefSeq Size: 4783 bp
• RefSeq ORF: 3915 bp
• Locus ID: 320405
• Cytogenetics: 6 A3.1
• Gene Summary: Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates neurotrophin release from granule cells leading to regulate cell differentiation and survival during cerebellar development. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles.[UniProtKB/Swiss-Prot Function]