Amhr2 (NM_144547) Mouse Tagged ORF Clone

CAT#: MG225496

Amhr2 (tGFP-tagged) - Mouse anti-Mullerian hormone type 2 receptor (Amhr2), (10ug)

Product Images

Circular map for MG225496

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Amhr2
• Synonyms: Misiir; Misrii; Mrii
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225496 representing NM_144547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGGACACTGGGGCTTTGGACACTGCTTCCTGCGGCAGCACAAGTATCCCCAAACAGGAGGACCT
  GTGTGTTCTTTGAGGCTCCTGGAGTGCGGGGAAGCACAAAGACACTGGGGGAAATGGTAGATGCAGGACC
  AGGCCCCCCCAAGGGTATCCGCTGCCTCTACAGCCATTGCTGCTTTGGGATCTGGAATCTTACCCATGGC
  CGGGCACAAGTGGAGATGCAAGGATGCCGAGACAGTGATGAGCCAGGCTGTGAGTCCCTCCACTGTGACC
  CAGTCCCCAGAGCCCACCCGAACCCCAGCTCCACACTCTTCACGTGTTCCTGTGGCACTGACTTCTGCAA
  TGCCAATTACAGCCATCTGCCTCCTTCGGGAAACCAAGGGGCTCCTGGCCCCCAGGAGCCCCAGGCTACC
  CCAGGTGGGCCCGTCTGGATGGCACTGCTGCTCCTGGGAATGTTTCTCGTGTTGTTGCTGAGTAGCATCA
  TCTTGGCCCTGCTACAACGAAAGGCCTGCAGAGTGCAAGGTGGGTCAGACCCAGAGCCCGGCTCAGGCGG
  AGACTGCAGTGAGGAACTGCCAGAGCTGGCCGAGCTGCGTTTCTCCCAGGTAATCCAGGAAGGAGGTCAT
  GCAGTCGTCTGGGCCGGGAGGCTTCAGGGTGAGATGGTAGCCATCAAGGCCTTCCCCCCAAGGGCTGTGG
  CCCAGTTCCGAGCTGAGAGGGCTGTGTACCAGCTGCTAGGCCTACAGCATGACCATATCGTTCGCTTTAT
  CACTGCTGGCCAGGGAGGCCCTGGCCCTCTGCCCTCTGGGCCTTTGCTGGTACTGGAACTGTACCCTAAG
  GGCTCCTTATGCCACTACTTGACTCAGTACACGAGCGACTGGGGAAGTTCCTTGAGGATGGCTCTGTCCC
  TGGCAGAGGGCTTGGCTTTTCTCCACGAGGAGCGTTGGCAGGATGGCCAGTACAAACCAGGTATCGCCCA
  CCGAGACCTGAGCAGCCAGAATGTGCTCATTCGGGAAGATAGGTCATGCGCCATCGGGGACCTGGGCCTT
  GCCTTGGTACTCCCTGGTCTTGCTCAGCCCCCTGCCTTGGCCCCTACTCAACCCCGAGGCCCCGCTGCCA
  TTTTGGAGGCTGGCACCCAGCGGTACATGGCTCCAGAGCTCTTGGACAAGACTCTAGACTTGCAGGACTG
  GGGTACAGCTCTCCAGAGAGCGGATGTTTACTCTCTGGCGCTACTACTGTGGGAGATCCTGAGCCGCTGT
  TCCGATTTGAGGCCTGACCACAGACCACCACCTTTCCAATTGGCTTATGAGGCAGAACTGGGCAGCAATC
  CCAGTGCCTGTGAGCTCTGGGCCCTGGCAGTGGAGGAGAGGAAGCGCCCCAACATCCCATCCACTTGGAG
  CTGCTCTGCCACAGACCCTCGGGGGCTGAGGGAGCTCCTGGAAGATTGCTGGGACGCAGACCCAGAAGCA
  CGGCTGACAGCTGAGTGTGTGCAGCAGCGCCTGGCAGCCCTGGCTTACCCTCACGGGGCGTCCTCTTTCC
  CAGAGAGTCCACAGGGCTGCCCAGAGAACTGCCTTTCAGCTCCCGCCTCTGCCGTTTTCCCCTGTCGGCC
  TCAGCAGAGTTCTTGTCTCCTCAGTGTTCAGCAAGGCCCTGGCTCTAGGAGTCCCGATCCTGTAGGCGAC
  ACTGTTCAGGTGTATGTAAATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225496 representing NM_144547
  Red=Cloning site Green=Tags(s)
  
  MLGTLGLWTLLPAAAQVSPNRRTCVFFEAPGVRGSTKTLGEMVDAGPGPPKGIRCLYSHCCFGIWNLTHG
  RAQVEMQGCRDSDEPGCESLHCDPVPRAHPNPSSTLFTCSCGTDFCNANYSHLPPSGNQGAPGPQEPQAT
  PGGPVWMALLLLGMFLVLLLSSIILALLQRKACRVQGGSDPEPGSGGDCSEELPELAELRFSQVIQEGGH
  AVVWAGRLQGEMVAIKAFPPRAVAQFRAERAVYQLLGLQHDHIVRFITAGQGGPGPLPSGPLLVLELYPK
  GSLCHYLTQYTSDWGSSLRMALSLAEGLAFLHEERWQDGQYKPGIAHRDLSSQNVLIREDRSCAIGDLGL
  ALVLPGLAQPPALAPTQPRGPAAILEAGTQRYMAPELLDKTLDLQDWGTALQRADVYSLALLLWEILSRC
  SDLRPDHRPPPFQLAYEAELGSNPSACELWALAVEERKRPNIPSTWSCSATDPRGLRELLEDCWDADPEA
  RLTAECVQQRLAALAYPHGASSFPESPQGCPENCLSAPASAVFPCRPQQSSCLLSVQQGPGSRSPDPVGD
  TVQVYVNE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144547
• ORF Size: 1704 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144547.2, NP_653130.2
• RefSeq Size: 1947 bp
• RefSeq ORF: 1707 bp
• Locus ID: 110542
• UniProt ID: Q8K592
• Cytogenetics: 15 57.58 cM
• Gene Summary: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone.[UniProtKB/Swiss-Prot Function]