Odf2 (NM_001177661) Mouse Tagged ORF Clone

CAT#: MG225492

Odf2 (tGFP-tagged) - Mouse outer dense fiber of sperm tails 2 (Odf2) transcript variant 5, (10ug)

Product Images

Circular map for MG225492

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Odf2
• Synonyms: AI848335; MMTEST29
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225492 representing NM_001177661
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGCTGCCGACCGGTTGTCGGAAAAGGAGGCGGAAGCGGGGAGGAGCCGCCGCGGGAGCCGGAC
  TGCATCCGCCGCGGCGTCTCCATGGCACGACCCTGGCCTCCGACTTCAACGACTTCATAAGGAGACGGTT
  CTGGGCGCAGCCGTGCCGCTCTTGGTTTCCATCGTGTGGGAAGAACGGAGTAACGAGTCTCACACAGAAA
  AAAGTCTTGAGAACACCTTGTGGCGCACCCAGTGTAACTGTTACGAAATCTCATAAGCGCGGAATGAAAG
  GGGACACCGTGAATGTACGGCGGAGTGTCCGGGTGAAAACCAAGGTACCTTGGATGCCCCCTGGAAAATC
  ATCTGCCCGGCATGTGGGATGCAAGTGGGAGAATCCACCTCATTGCCTGGAGATCACACCACCATCTTCA
  GAAAAGCTGGTCTCGGTGATGCGATTAAGTGACCTTTCTACAGAAGACGACGATTCAGGTCACTGTAAAA
  TGAACCGTTACGATAAGAAGATTGACAGTCTAATGAACGCGGTCGGTTGTCTCAAATCTGAGGTCAAGAT
  GCAGAAAGGTGAACGCCAAATGGCCAAAAGGTTCCTGGAGGAGAGGAAGGAAGAGCTGGAGGAGGTGGCA
  CACGAGCTTGCAGAGACGGAGCATGAGAACACGGTGCTCAGACACAACATCGAGCGTATCAAGGAGGAGA
  AGGACTTCACCATGCTTCAAAAGAAACACCTCCAGCAGGAGAAGGAATGCCTCATGTCCAAACTAGTGGA
  GGCTGAAATGGATGGAGCAGCTGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGAAGCTGAAA
  ACCGAGAAACAGATGACTTGCACCGACATCAACACCTTAACAAGGCAGAAGGAACTTCTCCTGCAGAAGC
  TGAGTACCTTTGAGGAGACCAATCGCACCCTCCGAGATCTGTTGAGGGAACAGCACTGCAAAGAGGATTC
  CGAGAGACTAATGGAGCAACAAGGTACATTACTGAAGCGTCTGGCAGAGGCAGACTCAGAGAAAGCGCGC
  CTGCTGTTACTACTGCAAGACAAGGACAAGGAGGTGGAAGAACTCCTCCAGGAGATACAATGTGAAAAGG
  CTCAAGCAAAGACAGCGTCTGAGCTTTCCAAGTCCATGGAGTCCATGCGGGGGCATTTGCAGGCACAGCT
  TCGCTGCAAAGAGGCTGAGAACAGTCGCCTGTGCATGCAGATCAAGAACTTAGAGCGCAGTGGGAACCAG
  CATAAGGCGGAAGTAGAGGCCATCATGGAGCAGCTAAAGGAACTGAAGCAGAAGGGAGACCGAGACAAAG
  AGACCCTAAAGAAGGCCATCCGAGCCCAGAAAGAGCGAGCCGAGAAGAGTGAGGAGTATGCCGAGCAGCT
  GCATGTGCAACTGGCCGACAAGGACCTTTATGTTGCTGAAGCTTTATCTACTCTGGAGTCTTGGAGGAGC
  CGTTACAACCAGGTTGTGAAGGACAAAGGAGATCTTGAGTTGGAGATTATCGTCCTAAATGACCGGGTGA
  CAGATCTTGTAAACCAACAACAAAGCTTGGAGGAGAAGATGCGGGAAGACCGGGACAGCTTGGTAGAGAG
  GCTGCACCGGCAGACGGCTGAGTACTCTGCATTCAAGCTAGAGAACGAGAGGCTCAAGGCTAGCTTTGCC
  CCTATGGAGGACAAGCTCAACCAGGCTCACTTGGAGGTTCAGCAGCTGAAGGCGTCAGTGAAGAACTACG
  AGGGGATGATAGACAACTACAAGAGCCAGGTGATGAAAACAAGATTGGAGGCTGATGAAGTGGCTGCACA
  GCTAGAGCGCTGCGACAAAGAGAACAAGATGCTTAAAGACGAGATGAACAAAGAGATCGAGGCGGCCCGT
  CGGCAGTTCCAGTCACAGCTGGCTGACCTACAACAGCTGCCTGACATCCTCAAGATCACGGAGGCCAAGC
  TGGCTGAGTGCCAAGACCAGCTGCAGGGCTACGAGAGGAAGAACATCGACCTCACAGCCATCATTTCAGA
  CCTGCGCAGCCGGGTAAGGGACTGGCAGAAGGGATCCCACGAACTGGCCCGAGCAGGGGCCCGCTTACCG
  AGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225492 representing NM_001177661
  Red=Cloning site Green=Tags(s)
  
  MGELPTGCRKRRRKRGGAAAGAGLHPPRRLHGTTLASDFNDFIRRRFWAQPCRSWFPSCGKNGVTSLTQK
  KVLRTPCGAPSVTVTKSHKRGMKGDTVNVRRSVRVKTKVPWMPPGKSSARHVGCKWENPPHCLEITPPSS
  EKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSEVKMQKGERQMAKRFLEERKEELEEVA
  HELAETEHENTVLRHNIERIKEEKDFTMLQKKHLQQEKECLMSKLVEAEMDGAAAAKQVMALKDTIGKLK
  TEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCKEDSERLMEQQGTLLKRLAEADSEKAR
  LLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHLQAQLRCKEAENSRLCMQIKNLERSGNQ
  HKAEVEAIMEQLKELKQKGDRDKETLKKAIRAQKERAEKSEEYAEQLHVQLADKDLYVAEALSTLESWRS
  RYNQVVKDKGDLELEIIVLNDRVTDLVNQQQSLEEKMREDRDSLVERLHRQTAEYSAFKLENERLKASFA
  PMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLERCDKENKMLKDEMNKEIEAAR
  RQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLRSRVRDWQKGSHELARAGARLP
  R
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177661
• ORF Size: 2103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177661.1, NP_001171132.1
• RefSeq Size: 2264 bp
• RefSeq ORF: 2106 bp
• Locus ID: 18286
• UniProt ID: A3KGV1
• Cytogenetics: 2 20.79 cM
• Gene Summary: Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly (By similarity).[UniProtKB/Swiss-Prot Function]