Odf2 (NM_001177659) Mouse Tagged ORF Clone

CAT#: MG225491

Odf2 (tGFP-tagged) - Mouse outer dense fiber of sperm tails 2 (Odf2) transcript variant 4, (10ug)

Product Images

Circular map for MG225491

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Odf2
• Synonyms: AI848335; MMTEST29
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225491 representing NM_001177659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGACCGATCTTCAACTCCCCCCTTACATGTTCACGTGGATGAGAACACTCCTGTCCACGTCCACA
  TAAAAAAACTCCCGAAACCGTCAGCAGCCAGCAGCCAGAAATCTCATAAGCGCGGAATGAAAGGGGACAC
  CGTGAATGTACGGCGGAGTGTCCGGGTGAAAACCAAGGTACCTTGGATGCCCCCTGGAAAATCATCTGCC
  CGGCATGTGGGATGCAAGTGGGAGAATCCACCTCATTGCCTGGAGATCACACCACCATCTTCAGAAAAGC
  TGGTCTCGGTGATGCGATTAAGTGACCTTTCTACAGAAGACGACGATTCAGGTCACTGTAAAATGAACCG
  TTACGATAAGAAGATTGACAGTCTAATGAACGCGGTCGGTTGTCTCAAATCTGAGGTCAAGATGCAGAAA
  GGTGAACGCCAAATGGCCAAAAGGTTCCTGGAGGAGAGGAAGGAAGAGCTGGAGGAGGTGGCACACGAGC
  TTGCAGAGACGGAGCATGAGAACACGGTGCTCAGACACAACATCGAGCGTATCAAGGAGGAGAAGGACTT
  CACCATGCTTCAAAAGAAACACCTCCAGCAGGAGAAGGAATGCCTCATGTCCAAACTAGTGGAGGCTGAA
  ATGGATGGAGCAGCTGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGAAGCTGAAAACCGAGA
  AACAGATGACTTGCACCGACATCAACACCTTAACAAGGCAGAAGGAACTTCTCCTGCAGAAGCTGAGTAC
  CTTTGAGGAGACCAATCGCACCCTCCGAGATCTGTTGAGGGAACAGCACTGCAAAGAGGATTCCGAGAGA
  CTAATGGAGCAACAAGGTACATTACTGAAGCGTCTGGCAGAGGCAGACTCAGAGAAAGCGCGCCTGCTGT
  TACTACTGCAAGACAAGGACAAGGAGGTGGAAGAACTCCTCCAGGAGATACAATGTGAAAAGGCTCAAGC
  AAAGACAGCGTCTGAGCTTTCCAAGTCCATGGAGTCCATGCGGGGGCATTGGCAGGCACAGCTTCGCTGC
  AAAGAGGCTGAGAACAGTCGCCTGTGCATGCAGATCAAGAACTTAGAGCGCAGTGGGAACCAGCATAAGG
  CGGAAGTAGAGGCCATCATGGAGCAGCTAAAGGAACTGAAGCAGAAGGGAGACCGAGACAAAGAGACGCT
  AAAGAAGGCCATCCGAGCCCAGAAAGAGCGAGCCGAGAAGAGTGAGGAGTATGCCGAGCAGCTGCATGTG
  CAACTGGCCGACAAGGACCTTTATGTTGCTGAAGCTTTATCTACTCTGGAGTCTTGGAGGAGCCGTTACA
  ACCAGGTTGTGAAGGACAAAGGAGATCTTGAGTTGGAGATTATCGTCCTAAATGACCGGGTGACAGATCT
  TGTAAACCAACAACAAAGCTTGGAGGAGAAGATGCGGGAAGACCGGGACAGCTTGGTAGAGAGGCTGCAC
  CGGCAGACGGCTGAGTACTCTGCATTCAAGCTAGAGAACGAGAGGCTCAAGGCTAGCTTTGCCCCTATGG
  AGGACAAGCTCAACCAGGCTCACTTGGAGGTTCAGCAGCTGAAGGCGTCAGTGAAGAACTACGAGGGGAT
  GATAGACAACTACAAGAGCCAGGTGATGAAAACAAGATTGGAGGCTGATGAAGTGGCTGCACAGCTAGAG
  CGCTGCGACAAAGAGAACAAGATGCTTAAAGACGAGATGAACAAAGAGATCGAGGCGGCCCGTCGGCAGT
  TCCAGTCACAGCTGGCTGACCTACAACAGCTGCCTGACATCCTCAAGATCACGGAGGCCAAGCTGGCTGA
  GTGCCAAGACCAGCTGCAGGGCTACGAGAGGAAGAACATCGACCTCACAGCCATCATTTCAGACCTGCGC
  AGCCGGATCGAACATCAGGGGGACAAGCTGGAGATGGCGAGAGAGAAACATCAGGCTTCCCAGAAGGAAA
  ATAAACAGCTGAGTCAGAAGGTGGATGAACTTGAGAGGAAACTGGAGGCTACCAGCGCCCAGAATGTCGA
  GTTCCTACAGGTGATTGCCAAGAGGGAGGAGGCAATCCACCAGGCTCAGCTGCGGTTGGAGGAGAAAACG
  AGGGAGTGTGGGTCATTGGCGAGGCAGTTGGAGAGTGCCATTGAAGATGCCAGGCGACAGGTGGAGCAAA
  CCAAGGAACAGGCACTCTCCAAGGAACGAGCAGCCCAGAGCAAAATCCTGGACCTTGAGACTCAGCTGAG
  CAGGACCAAGACAGAACTTGGCCAGCTGCGGCGGACTCGTGATGATGCGGATCGCAGATACCAGAGCCGC
  CTGCAGGATCTGAAAGATCGCCTGGAGCAGTCTGAGAGTACCAACCGCAGCATGCAGAATTATGTCCAAT
  TCCTCAAGGCCTCTTATGCAAATGTGTTTGGGGATGCTCCCTATACATCCTCCTACCTGACGAGCTCACC
  CATCCGCTCCCGATCGCCCCCTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225491 representing NM_001177659
  Red=Cloning site Green=Tags(s)
  
  MKDRSSTPPLHVHVDENTPVHVHIKKLPKPSAASSQKSHKRGMKGDTVNVRRSVRVKTKVPWMPPGKSSA
  RHVGCKWENPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSEVKMQK
  GERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERIKEEKDFTMLQKKHLQQEKECLMSKLVEAE
  MDGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCKEDSER
  LMEQQGTLLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHWQAQLRC
  KEAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKETLKKAIRAQKERAEKSEEYAEQLHV
  QLADKDLYVAEALSTLESWRSRYNQVVKDKGDLELEIIVLNDRVTDLVNQQQSLEEKMREDRDSLVERLH
  RQTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLE
  RCDKENKMLKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLR
  SRIEHQGDKLEMAREKHQASQKENKQLSQKVDELERKLEATSAQNVEFLQVIAKREEAIHQAQLRLEEKT
  RECGSLARQLESAIEDARRQVEQTKEQALSKERAAQSKILDLETQLSRTKTELGQLRRTRDDADRRYQSR
  LQDLKDRLEQSESTNRSMQNYVQFLKASYANVFGDAPYTSSYLTSSPIRSRSPPA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177659
• ORF Size: 2478 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177659.1, NP_001171130.1
• RefSeq Size: 3919 bp
• RefSeq ORF: 2481 bp
• Locus ID: 18286
• UniProt ID: A3KGV1
• Cytogenetics: 2 20.79 cM
• Gene Summary: Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly (By similarity).[UniProtKB/Swiss-Prot Function]