Evl (NM_001163394) Mouse Tagged ORF Clone

CAT#: MG225304

Evl (tGFP-tagged) - Mouse Ena-vasodilator stimulated phosphoprotein (Evl) transcript variant 1, (10ug)

Product Images

Circular map for MG225304

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Evl
• Synonyms: AI528774; b2b2600Clo
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225304 representing NM_001163394
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAACAGAGTATCTGCCAAGCGCGGGCCTCCGTGATGGTCTACGATGACACCAGTAAGAAGTGGG
  TACCGATCAAGCCTGGCCAGCAGGGATTCAGCCGGATCAACATCTACCACAACACTGCCAGCAGCACCTT
  CAGAGTGGTCGGGGTCAAGCTACAGGACCAGCAGGTTGTGATCAATTATTCAATTGTTAAAGGGCTGAAG
  TACAATCAGGCAACACCCACCTTCCATCAGTGGCGAGATGCCCGTCAGGTCTATGGCTTAAACTTTGCAA
  GTAAGGAAGAAGCAACCACATTCTCCAATGCCATGCTCTTTGCCCTGAACATCATGAATTCCCAAGAAGG
  AGGCCCCTCCACACAGCGTCAGGTGCAGAATGGCCCCTCTCCTGAGGAGATGGACATCCAGAGAAGACAA
  GTAATGGAGCAGCAGCACCGCCAGGAGTCTCTGGAGAGGAGAATCTCGGCCACAGGGCCCATTCTCCCCC
  CTGGGCATCCCTCATCGGCAGCCAGCACCACTCTCTCCTGTAGTGGACCTCCACCCCCGCCTCCACCCCC
  AGTTCCACCTCCACCCACAGGGTCTACTCCCCCACCCCCACCCCCACTGCCAGCTGGAGGAGCCCAGGGG
  ACCAACCATGATGAGAGCTCTGCATCAGGACTGGCTGCTGCTCTGGCGGGAGCCAAGCTAAGGAGGGTGC
  AGCGGCCAGAAGATGCATCTGGAGGCTCCAGTCCTAGTGGGACTTCAAAGTCCGATGCCAACCGGGCAAG
  CAGTGGGGGAGGTGGAGGAGGCCTCATGGAAGAAATGAACAAGCTGCTGGCTAAGAGGAGAAAGGCAGCC
  TCCCAGACAGACAAGCCCGCTGACAGAAAGGAAGATGAGAGCCAAACGGAAGACCCTAGCACCTCCCCAT
  CCCCAGGTACCCGAGCCACCAGCCAGCCACCTAATTCCTCAGAGGCTGGCAGAAAACCCTGGGAACGGAG
  CAACTCGGTGGAGAAACCTGTGTCCTCGTTGCTGTCCAGAACCCCGTCTGTGGCAAAGAGCCCCGAAGCT
  AAGAGCCCCCTTCAGTCGCAGCCTCACTCTAGGGTGAAGCCTGCTGGGAGTGTGAATGACGTGGGCCTGG
  ATGCCTTAGATTTGGACCGGATGAAACAGGAGATCCTGGAGGAGGTGGTTCGGGAGCTGCACAAGGTGAA
  GGAGGAGATCATTGATGCCATCAGGCAGGAGCTAAGTGGGATCAGCACCACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225304 representing NM_001163394
  Red=Cloning site Green=Tags(s)
  
  MSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTASSTFRVVGVKLQDQQVVINYSIVKGLK
  YNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGGPSTQRQVQNGPSPEEMDIQRRQ
  VMEQQHRQESLERRISATGPILPPGHPSSAASTTLSCSGPPPPPPPPVPPPPTGSTPPPPPPLPAGGAQG
  TNHDESSASGLAAALAGAKLRRVQRPEDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNKLLAKRRKAA
  SQTDKPADRKEDESQTEDPSTSPSPGTRATSQPPNSSEAGRKPWERSNSVEKPVSSLLSRTPSVAKSPEA
  KSPLQSQPHSRVKPAGSVNDVGLDALDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163394
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163394.1, NP_001156866.1
• RefSeq Size: 2202 bp
• RefSeq ORF: 1245 bp
• Locus ID: 14026
• UniProt ID: P70429
• Cytogenetics: 12 F1
• Gene Summary: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.[UniProtKB/Swiss-Prot Function]