Evl (NM_001163394) Mouse Untagged Clone

CAT#: MC208457

Evl (untagged) - Mouse Ena-vasodilator stimulated phosphoprotein (Evl), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Evl
• Synonyms: AI528774; b2b2600Clo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208457 representing NM_001163394
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAACAGAGTATCTGCCAAGCGCGGGCCTCCGTGATGGTCTACGATGACACCAGTAAGAAGTGGG
  TACCGATCAAGCCTGGCCAGCAGGGATTCAGCCGGATCAACATCTACCACAACACTGCCAGCAGCACCTT
  CAGAGTGGTCGGGGTCAAGCTACAGGACCAGCAGGTTGTGATCAATTATTCAATTGTTAAAGGGCTGAAG
  TACAATCAGGCAACACCCACCTTCCATCAGTGGCGAGATGCCCGTCAGGTCTATGGCTTAAACTTTGCAA
  GTAAGGAAGAAGCAACCACATTCTCCAATGCCATGCTCTTTGCCCTGAACATCATGAATTCCCAAGAAGG
  AGGCCCCTCCACACAGCGTCAGGTGCAGAATGGCCCCTCTCCTGAGGAGATGGACATCCAGAGAAGACAA
  GTAATGGAGCAGCAGCACCGCCAGGAGTCTCTGGAGAGGAGAATCTCGGCCACAGGGCCCATTCTCCCCC
  CTGGGCATCCCTCATCGGCAGCCAGCACCACTCTCTCCTGTAGTGGACCTCCACCCCCGCCTCCACCCCC
  AGTTCCACCTCCACCCACAGGGTCTACTCCCCCACCCCCACCCCCACTGCCAGCTGGAGGAGCCCAGGGG
  ACCAACCATGATGAGAGCTCTGCATCAGGACTGGCTGCTGCTCTGGCGGGAGCCAAGCTAAGGAGGGTGC
  AGCGGCCAGAAGATGCATCTGGAGGCTCCAGTCCTAGTGGGACTTCAAAGTCCGATGCCAACCGGGCAAG
  CAGTGGGGGAGGTGGAGGAGGCCTCATGGAAGAAATGAACAAGCTGCTGGCTAAGAGGAGAAAGGCAGCC
  TCCCAGACAGACAAGCCCGCTGACAGAAAGGAAGATGAGAGCCAAACGGAAGACCCTAGCACCTCCCCAT
  CCCCAGGTACCCGAGCCACCAGCCAGCCACCTAATTCCTCAGAGGCTGGCAGAAAACCCTGGGAACGGAG
  CAACTCGGTGGAGAAACCTGTGTCCTCGTTGCTGTCCAGAACCCCGTCTGTGGCAAAGAGCCCCGAAGCT
  AAGAGCCCCCTTCAGTCGCAGCCTCACTCTAGGGTGAAGCCTGCTGGGAGTGTGAATGACGTGGGCCTGG
  ATGCCTTAGATTTGGACCGGATGAAACAGGAGATCCTGGAGGAGGTGGTTCGGGAGCTGCACAAGGTGAA
  GGAGGAGATCATTGATGCCATCAGGCAGGAGCTAAGTGGGATCAGCACCACGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163394
• Insert Size: 1245 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163394.1, NP_001156866.1
• RefSeq Size: 2202 bp
• RefSeq ORF: 1245 bp
• Locus ID: 14026
• UniProt ID: P70429
• Cytogenetics: 12 F1
• Gene Summary: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).