Pou3f2 (NM_008899) Mouse Tagged ORF Clone

CAT#: MG225266

Pou3f2 (tGFP-tagged) - Mouse POU domain class 3 transcription factor 2 (Pou3f2), (10ug)

Product Images

Circular map for MG225266

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pou3f2
• Synonyms: 9430075J19Rik; A230098E07Rik; Brn-2; Brn2; oct-7; OTF-7; Otf7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225266 representing NM_008899
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGCAGCGTCTAACCACTACAGCCTGCTCACCTCCAGCGCCTCCATCGTACATGCCGAGCCGC
  CTGGCGGCATGCAGCAGGGCGCAGGGGGCTACCGCGAGGCGCAGAGCCTGGTGCAGGGCGACTACGGCGC
  GCTGCAGAGCAACGGGCACCCGCTCAGCCACGCTCACCAGTGGATCACCGCGCTGTCCCACGGCGGCGGC
  GGCGGGGGCGGCGGCGGCGGTGGAGGAGGCGGGGGAGGCGGCGGGGGAGGCGGCGACGGCTCCCCGTGGT
  CCACCAGCCCCCTAGGCCAGCCGGACATCAAGCCCTCGGTGGTGGTACAGCAGGGTGGCCGAGGCGACGA
  GCTGCACGGGCCAGGAGCGCTGCAGCAACAGCATCAACAGCAACAGCAACAGCAGCAGCAGCAGCAGCAG
  CAGCAGCAGCAGCAACAGCAGCAGCAACAACAGCGACCGCCACATCTGGTGCACCACGCTGCCAACCACC
  ATCCCGGGCCCGGGGCATGGCGGAGTGCGGCGGCTGCAGCTCACCTCCCTCCCTCCATGGGAGCTTCCAA
  CGGCGGTTTGCTCTATTCGCAGCCGAGCTTCACGGTGAACGGCATGCTGGGCGCAGGAGGGCAGCCGGCT
  GGGCTGCACCACCACGGCCTGAGGGACGCCCACGATGAGCCACACCATGCAGACCACCACCCGCATCCGC
  ACTCTCACCCACACCAGCAACCGCCCCCGCCACCTCCCCCACAAGGCCCACCGGGCCACCCAGGCGCGCA
  CCACGACCCGCACTCGGACGAGGACACGCCGACCTCAGACGACCTGGAGCAGTTCGCCAAGCAATTCAAG
  CAGAGGCGGATCAAACTCGGATTTACTCAAGCAGACGTGGGGCTGGCGCTTGGCACCCTGTACGGCAACG
  TGTTCTCGCAGACCACCATCTGCAGGTTTGAGGCCCTGCAGCTGAGCTTCAAGAACATGTGCAAGCTGAA
  GCCTTTGTTGAACAAGTGGTTGGAAGAGGCAGACTCATCCTCGGGCAGCCCCACCAGCATAGACAAGATC
  GCAGCGCAAGGGCGCAAACGGAAAAAGCGGACCTCCATCGAGGTGAGCGTCAAGGGGGCTCTGGAGAGCC
  ATTTCCTCAAATGCCCTAAGCCCTCGGCCCAGGAGATCACCTCCCTCGCGGACAGCTTACAGCTGGAGAA
  GGAGGTGGTGAGAGTTTGGTTTTGTAACAGGAGACAGAAAGAGAAAAGGATGACCCCTCCCGGAGGGACT
  CTGCCGGGCGCCGAGGATGTGTATGGGGGTAGTAGGGACACGCCACCACACCACGGGGTGCAGACGCCCG
  TCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225266 representing NM_008899
  Red=Cloning site Green=Tags(s)
  
  MATAASNHYSLLTSSASIVHAEPPGGMQQGAGGYREAQSLVQGDYGALQSNGHPLSHAHQWITALSHGGG
  GGGGGGGGGGGGGGGGGGDGSPWSTSPLGQPDIKPSVVVQQGGRGDELHGPGALQQQHQQQQQQQQQQQQ
  QQQQQQQQQQQRPPHLVHHAANHHPGPGAWRSAAAAAHLPPSMGASNGGLLYSQPSFTVNGMLGAGGQPA
  GLHHHGLRDAHDEPHHADHHPHPHSHPHQQPPPPPPPQGPPGHPGAHHDPHSDEDTPTSDDLEQFAKQFK
  QRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSSGSPTSIDKI
  AAQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRMTPPGGT
  LPGAEDVYGGSRDTPPHHGVQTPVQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008899
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008899.2, NP_032925.1
• RefSeq Size: 1338 bp
• RefSeq ORF: 1338 bp
• Locus ID: 18992
• UniProt ID: P31360
• Cytogenetics: 4 9.73 cM
• Gene Summary: Transcription factor that plays a key role in neuronal differentiation (PubMed:24243019). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (PubMed:24243019).[UniProtKB/Swiss-Prot Function]