Axin1 (NM_009733) Mouse Tagged ORF Clone

CAT#: MG225159

Axin1 (tGFP-tagged) - Mouse axin 1 (Axin1) transcript variant 2, (10ug)

Product Images

Circular map for MG225159

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Axin1
• Synonyms: AI316800; Axin; Fu; fused; Kb; Ki; kinky; knobbly
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225159 representing NM_009733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGTCCCAAAATGAATGTCCAGGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAG
  ATGCCCCCCGGCCCCCAGTGCCTGGAGAAGAGGGAGAACTGGTATCTACTGATTCGAGGCCTGTCAACCA
  CAGTTTCTGTTCTGGGAAAGGTACCAGCATTAAAAGTGAGACCTCAACAGCCACCCCAAGACGTTCAGAT
  CTGGATCTGGGATATGAGCCCGAGGGCAGTGCCTCCCCCACCCCACCATATTTGAGGTGGGCTGAGTCAC
  TGCATTCCTTACTGGATGACCAAGATGGGATCAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGC
  TGACCTGCTGGACTTCTGGTTTGCCTGCAGTGGCTTCAGGAAGCTTGAGCCCTGTGACTCAAATGAGGAA
  AAGAGGCTGAAGCTGGCAAGAGCCATCTACCGAAAGTACATCCTGGATAGCAATGGCATTGTGTCCAGAC
  AAACCAAGCCAGCCACTAAGAGCTTCATAAAGGACTGTGTCATGAAGCAGCAGATAGATCCTGCCATGTT
  TGACCAGGCACAGACAGAAATCCAGTCCACCATGGAGGAGAATACCTACCCTTCCTTTCTTAAGTCTGAC
  ATTTATTTGGAGTACACAAGGACAGGCTCAGAGAGTCCGAAGGTCTGCAGTGACCAGAGCTCAGGGTCTG
  GAACAGGGAAGGGCATGTCTGGATACCTGCCCACTTTGAATGAGGATGAAGAATGGAAATGTGACCAAGA
  TGCAGATGAGGATGATGGCCGAGACCCTCTCCCCCCCAGCAGGCTCACCCAGAAGCTGCTATTGGAGACT
  GCTGCCCCGAGGGCCCCCTCAAGTAGACGGTACAACGAAGGCAGAGAGCTCAGGTATGGATCTTGGAGGG
  AGCCCGTCAACCCCTACTACGTCAACTCTGGCTATGCCCTGGCCCCAGCCACCAGTGCCAATGACAGTGA
  GCAGCAGAGCCTGTCCAGTGATGCTGACACGCTATCCCTTACGGACAGTAGTGTGGATGGAATCCCCCCA
  TACAGGATCCGTAAGCAGCACCGAAGGGAGATGCAGGAGAGTATCCAAGTCAATGGGCGGGTACCTCTAC
  CTCACATTCCTCGCACTTACCGAATGCCAAAGGAGATCCGGGTAGAGCCACAGAAATTTGCTGAAGAGCT
  TATTCACCGTCTAGAGGCTGTCCAGCGCACTCGAGAGGCTGAAGAAAAGTTGGAGGAACGGCTGAAGCGT
  GTACGCATGGAGGAAGAAGGGGAGGATGGTGAAATGCCTTCTGGCCCCATGGCAAGTCACAAGCTGCCTT
  CTGTCCCAGCTTGGCACCATTTCCCACCCCGCTATGTGGATATGGGCTGCTCTGGACTGCGGGATGCCCA
  TGAGGAGAATCCTGAGAGCATCCTGGATGAGCACGTGCAAAGGGTCATGAGGACACCTGGCTGCCAGTCA
  CCTGGCCCAGGCCACCGCTCTCCTGACAGTGGGCATGTGGCTAAGACTGCAGTGCTAGGGGGTACAGCCT
  CCGGGCATGGGAAGCATGTTCCTAAGTTAGGGTTGAAGCTGGATACAGCTGGCCTGCACCATCATAGACA
  TGTCCACCACCATGTTCACCATAATTCAGCTAGACCTAAGGAGCAAATGGAGGCTGAAGTTGCCCGCAGG
  GTCCAGAGCAGCTTCTCGTGGGGCCCAGAAACACATGGTCATGCCAAGCCCCGGAGCTATTCCGAGAACG
  CAGGCACCACCCTCAGTGCTGGGGATTTGGCCTTTGGTGGTAAAACTAGTGCACCTTCCAAAAGAAACAC
  CAAGAAGGCTGAATCTGGGAAGAATGCCAATGCTGAGGTACCCAGTACCACAGAGGACGCTGAGAAGAAC
  CAGAAGATCATGCAGTGGATCATTGAGGGAGAGAAGGAGATCAGTAGACACCGGAAGGCAGGCCATGGGT
  CTTCTGGGTTGAGGAAGCAGCAGGCCCATGAAAGCTCCAGGCCCTTGTCCATCGAGCGTCCTGGGGCCGT
  GCACCCCTGGGTCAGCGCTCAGCTTCGGAATTCTGTCCAGCCTTCTCATCTTTTCATCCAAGATCCCACA
  ATGCCACCCAATCCAGCCCCTAATCCCCTGACCCAGCTGGAAGAGGCCCGCAGGCGTTTGGAAGAAGAAG
  AAAAGAGAGCAAACAAACTGCCCTCCAAGCAGAGGTATGTGCAGGCAGTCATGCAGCGGGGACGCACCTG
  TGTCAGGCCAGCTTGTGCACCGGTGCTGAGTGTGGTACCAGCCGTGTCGGACTTGGAACTCTCCGAGACA
  GAGACAAAATCACAAAGAAAGGCAGGTGGCGGGAGTGCACCACCATGTGACAGCATTGTTGTGGCCTACT
  ATTTCTGTGGGGAACCCATCCCCTACCGGACCCTGGTGAGGGGCCGTGCTGTCACCCTGGGCCAGTTCAA
  GGAGCTGCTAACCAAGAAGGGGAGCTACAGATACTACTTTAAGAAAGTGAGTGATGAGTTTGACTGTGGT
  GTGGTATTTGAGGAAGTACGGGAGGATGAGGCCGTCTTGCCTGTCTTTGAAGAAAAGATCATCGGCAAGG
  TGGAAAAGGTGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225159 representing NM_009733
  Red=Cloning site Green=Tags(s)
  
  MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSD
  LDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEE
  KRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSD
  IYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLET
  AAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPP
  YRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKR
  VRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQS
  PGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARR
  VQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKN
  QKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPT
  MPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRYVQAVMQRGRTCVRPACAPVLSVVPAVSDLELSET
  ETKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCG
  VVFEEVREDEAVLPVFEEKIIGKVEKVD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009733
• ORF Size: 2604 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009733.2, NP_033863.2
• RefSeq Size: 3809 bp
• RefSeq ORF: 2607 bp
• Locus ID: 12005
• UniProt ID: O35625
• Cytogenetics: 17 13.07 cM
• Gene Summary: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.[UniProtKB/Swiss-Prot Function]