Asph (NM_001177849) Mouse Tagged ORF Clone

CAT#: MG225135

Asph (tGFP-tagged) - Mouse aspartate-beta-hydroxylase (Asph) transcript variant 3, (10ug)

Product Images

Circular map for MG225135

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Asph
• Synonyms: 2310005F16Rik; 3110001L23Rik; AI848629; AW261690; AW561901; BAH; C79816; cI-37
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225135 representing NM_001177849
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGCGCAAGAACGCCAAGGGCGGCGGCGGCAACAGCAGCAGCAGCGGCAGCGGCAGCGGCTCGG
  GCAGCGGTAGCCCGAGCACGGGTAGCAGCGGTAGCAGCAGTAGCCCCGGGGCTCGGAGAGAGGCAAAGCA
  CGGAGGACACAAGAATGGGAGAAGAGGAGGGATTTCAGGAGGGTCCTTTTTCACATGGTTCATGGTCATT
  GCATTGCTCGGCGTCTGGACATCTGTGGCTGTCGTGTGGTTTGACTTGGTCGATTATGAAGAAGTTCTAG
  GAAAACTAGGAGTCTATGATGCGGATGGCGATGGAGACTTTGATGTGGATGATGCCAAAGTTTTATTAGG
  CCTTAAAGAAAGATCTCCTTCTGAGAGGACATTTCCACCGGAGGAGGAGGCAGAGACTCACGCTGAGCTG
  GAGGAGCAGGCCCCTGAGGGGGCAGACATCCAGAATGTTGAAGATGAAGTAAAGGAACAAATTCAGTCCC
  TTCTTCAGGAATCAGTACACACAGACCATGACCTTGAAGCAGATGGGCTGGCAGGAGAACCACAGCCGGA
  GGTTGAGGACTTCCTCACAGTGACCGACAGTGATGACAGATTTGAGGACCTGGAACCCGGGACAGTTCAT
  GAAGAAATCGAGGATACTTACCATGTGGAAGACACAGCATCGCAGAACCATCCAAATGACATGGAAGAGA
  TGACGAATGAGCAGGAAAATTCAGAGGAAGTAAGACACCAAGACTATGATGAACCAGTATATGAACCCTC
  AGAGCATGAAGGGGTCGCGATTTCAGATAACACCATAGATGATTCCAGCATAATCTCAGAAGAAATAAAT
  GTCGCCTCTGTTGAAGAACAGCAAGACACACCACCAGTTAAGAAGAAGAAGCCTAAACTTCTGAACAAAT
  TTGATAAGACAATTAAGGCTGAGCTGGATGCTGCAGAAAAGCTCCGGAAAAGGGGTAAAATTGAGGAAGC
  AGTGAACGCATTTGAAGAACTGGTTCGAAAGTACCCTCAGAGCCCACGAGCAAGATATGGCAAAGCGCAG
  TGTGAAGATGACTTGGCAGAGAAGCAGAGAAGCAATGAGGTTCTGCGCAGGGCCATCGAGACCTACCAGG
  AGGCAGCCGACCTGCCTGATGCCCCTACAGACCTGGTGAAGCTGAGCTTGAAGCGAAGGTCGGAACGGCA
  GCAGTTTCTAGGTCACATGAGAGGTTCTCTACTTACCCTCCAGAGACTAGTTCAACTGTTCCCTAGTGAT
  ACTACCTTAAAAAACGACCTTGGCGTAGGATACCTCTTGTTGGGAGACAACGACAGTGCCAAGAAGGTTT
  ACGAAGAGGTGCTAAATGTGACACCAAATGATGGCTTCGCTAAAGTGCATTACGGCTTCATCCTGAAGGC
  ACAGAACAAGATATCTGAGAGCATTCCCTACTTAAAGGAAGGAATCGAATCTGGGGACCCTGGCACGGAT
  GATGGCCGGTTTTACTTCCACTTGGGGGATGCCATGCAGAGGGTCGGGAACAAAGAGGCATATAAGTGGT
  ATGAACTTGGGCACAAGAGAGGACATTTTGCCTCTGTCTGGCAGCGTTCCCTCTACAATGTGAATGGTCT
  GAAGGCTCAGCCGTGGTGGACACCCAGGGAGACTGGCTACACAGAGCTAGTGAAGTCTTTAGAGAGAAAC
  TGGAAGTTAATCCGTGATGAAGGCCTCATGGTGATGGATAAAGCCAAGGGTCTCTTCCTGCCTGAGGACG
  AAAACCTTCGGGAGAAGGGCGACTGGAGCCAGTTCACACTGTGGCAGCAAGGAAGGAAGAATGAGAATGC
  CTGTAAAGGAGCGCCTAAGACCTGTGCTTTACTAGAAAAGTTTTCCGAAACAACAGGATGCAGAAGAGGA
  CAGATCAAATACTCCATCATGCACCCTGGAACTCATGTGTGGCCGCATACAGGACCCACAAACTGCAGGC
  TCCGAATGCATCTGGGGTTAGTGATCCCCAAGGAAGGCTGCAAGATCCGGTGTGCCAATGAGACCAGGAC
  GTGGGAAGAAGGCAAGGTGCTCATCTTTGATGACTCTTTTGAGCACGAGGTTTGGCAGGATGCCTCGTCT
  TTCCGGCTGATATTCATCGTGGATGTGTGGCACCCCGAGCTGACCCCTCAGCAGAGACGCAGCCTTCCCG
  CAATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225135 representing NM_001177849
  Red=Cloning site Green=Tags(s)
  
  MAPRKNAKGGGGNSSSSGSGSGSGSGSPSTGSSGSSSSPGARREAKHGGHKNGRRGGISGGSFFTWFMVI
  ALLGVWTSVAVVWFDLVDYEEVLGKLGVYDADGDGDFDVDDAKVLLGLKERSPSERTFPPEEEAETHAEL
  EEQAPEGADIQNVEDEVKEQIQSLLQESVHTDHDLEADGLAGEPQPEVEDFLTVTDSDDRFEDLEPGTVH
  EEIEDTYHVEDTASQNHPNDMEEMTNEQENSEEVRHQDYDEPVYEPSEHEGVAISDNTIDDSSIISEEIN
  VASVEEQQDTPPVKKKKPKLLNKFDKTIKAELDAAEKLRKRGKIEEAVNAFEELVRKYPQSPRARYGKAQ
  CEDDLAEKQRSNEVLRRAIETYQEAADLPDAPTDLVKLSLKRRSERQQFLGHMRGSLLTLQRLVQLFPSD
  TTLKNDLGVGYLLLGDNDSAKKVYEEVLNVTPNDGFAKVHYGFILKAQNKISESIPYLKEGIESGDPGTD
  DGRFYFHLGDAMQRVGNKEAYKWYELGHKRGHFASVWQRSLYNVNGLKAQPWWTPRETGYTELVKSLERN
  WKLIRDEGLMVMDKAKGLFLPEDENLREKGDWSQFTLWQQGRKNENACKGAPKTCALLEKFSETTGCRRG
  QIKYSIMHPGTHVWPHTGPTNCRLRMHLGLVIPKEGCKIRCANETRTWEEGKVLIFDDSFEHEVWQDASS
  FRLIFIVDVWHPELTPQQRRSLPAI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177849
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177849.1, NP_001171320.1
• RefSeq Size: 6640 bp
• RefSeq ORF: 2178 bp
• Locus ID: 65973
• Cytogenetics: 4 A1
• Gene Summary: Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.[UniProtKB/Swiss-Prot Function]