Canx (NM_001110500) Mouse Tagged ORF Clone

CAT#: MG225122

Canx (tGFP-tagged) - Mouse calnexin (Canx) transcript variant 3, (10ug)

Product Images

Circular map for MG225122

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Canx
• Synonyms: 1110069N15Rik; AI988026; Cnx; D11Ertd153e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225122 representing NM_001110500
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGAAGTGGTTACTGTGTTTGCTGCTGGTCCTTGGAACTGCAGCTGTTGAGGCTCATGATGGAC
  ATGATGATGACGCGATTGATATTGAAGATGATCTTGATGATGTTATTGAAGAGGTAGAAGATTCAAAATC
  TAAATCAGATGCCAGCACTCCTCCATCTCCAAAGGTCACCTACAAAGCTCCAGTTCCAACAGGGGAGGTT
  TATTTTGCTGACTCCTTTGACAGAGGGTCTCTGTCAGGGTGGATTTTATCTAAAGCCAAAAAAGATGACA
  CTGATGATGAAATTGCCAAATATGATGGAAAGTGGGAAGTAGATGAGATGAAGGAAACAAAGCTTCCAGG
  GGATAAAGGACTTGTACTGATGTCTCGGGCCAAGCATCATGCCATCTCTGCTAAACTGAATAAGCCCTTC
  CTGTTTGATACCAAGCCTCTCATTGTTCAGTATGAGGTTAATTTTCAGAATGGAATAGAATGTGGTGGTG
  CCTATGTGAAGCTGCTTTCCAAGACGGCAGAGCTCAGCCTGGATCAATTCCACGACAAGACTCCCTATAC
  TATTATGTTTGGTCCAGATAAGTGTGGAGAGGACTACAAACTGCATTTCATCTTTCGACACAAAAATCCC
  AAGACAGGTGTATATGAAGAAAAACATGCTAAGAGGCCAGATGCAGATCTGAAGACCTATTTCACTGACA
  AGAAAACGCATCTTTATACATTAATCTTGAATCCAGACAATAGTTTTGAAATATTAGTTGACCAGTCTGT
  TGTGAACAGTGGAAATCTGCTAAATGACATGACTCCTCCTGTAAACCCTTCACGTGAAATTGAAGACCCA
  GAAGACCGGAAGCCTGAAGATTGGGATGAAAGGCCCAAAATAGCAGATCCAGATGCTGTCAAGCCAGATG
  ACTGGGATGAAGACGCCCCTTCTAAGATCCCAGATGAAGAGGCCACCAAGCCTGAAGGCTGGCTAGACGA
  CGAACCTGAGTATATTCCAGACCCTGATGCAGAGAAGCCAGAGGATTGGGATGAGGATATGGACGGAGAA
  TGGGAGGCTCCTCAGATTGCCAACCCCAAGTGTGAGTCAGCCCCTGGGTGTGGTGTCTGGCAGCGACCTA
  TGATTGACAACCCCAATTATAAGGGCAAATGGAAGCCTCCAATGATTGACAACCCTAACTACCAGGGAAT
  CTGGAAACCAAGGAAAATACCAAATCCAGATTTCTTTGAAGACCTAGAACCTTTTAAGATGACTCCTTTC
  AGTGCTATTGGTTTGGAGCTCTGGTCCATGACATCCGACATCTTTTTTGACAACTTTATCATTAGTGGTG
  ACCGAAGAGTAGTTGATGATTGGGCCAATGATGGGTGGGGCCTGAAGAAAGCTGCTGATGGGGCTGCTGA
  GCCAGGTGTAGTGCTGCAGATGCTGGAGGCAGCTGAAGAGCGTCCATGGCTTTGGGTGGTCTACATTTTG
  ACTGTAGCTTTGCCAGTGTTCCTTGTGATCCTCTTCTGCTGTTCTGGAAAGAAACAGTCCAATGCTATGG
  AGTACAAGAAGACGGATGCTCCCCAGCCAGATGTGAAGGATGAAGAAGGGAAGGAAGAAGAGAAGAACAA
  GAGGGATGAAGAGGAAGAAGAGGAGAAGCTTGAAGAGAAACAGAAGAGTGATGCTGAAGAAGATGGTGTT
  ACTGGCAGTCAAGATGAGGAAGATAGCAAGCCTAAAGCAGAGGAGGATGAAATTTTGAACAGATCGCCAA
  GAAACAGAAAGCCACGAAGAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225122 representing NM_001110500
  Red=Cloning site Green=Tags(s)
  
  MEGKWLLCLLLVLGTAAVEAHDGHDDDAIDIEDDLDDVIEEVEDSKSKSDASTPPSPKVTYKAPVPTGEV
  YFADSFDRGSLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKETKLPGDKGLVLMSRAKHHAISAKLNKPF
  LFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTAELSLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNP
  KTGVYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSVVNSGNLLNDMTPPVNPSREIEDP
  EDRKPEDWDERPKIADPDAVKPDDWDEDAPSKIPDEEATKPEGWLDDEPEYIPDPDAEKPEDWDEDMDGE
  WEAPQIANPKCESAPGCGVWQRPMIDNPNYKGKWKPPMIDNPNYQGIWKPRKIPNPDFFEDLEPFKMTPF
  SAIGLELWSMTSDIFFDNFIISGDRRVVDDWANDGWGLKKAADGAAEPGVVLQMLEAAEERPWLWVVYIL
  TVALPVFLVILFCCSGKKQSNAMEYKKTDAPQPDVKDEEGKEEEKNKRDEEEEEEKLEEKQKSDAEEDGV
  TGSQDEEDSKPKAEEDEILNRSPRNRKPRRE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110500
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110500.1, NP_001103970.1
• RefSeq Size: 4256 bp
• RefSeq ORF: 1776 bp
• Locus ID: 12330
• UniProt ID: P35564
• Cytogenetics: 11 30.46 cM
• Gene Summary: Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.[UniProtKB/Swiss-Prot Function]