Trpm3 (NM_001035244) Mouse Tagged ORF Clone

CAT#: MG225067

Trpm3 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 3 (Trpm3) transcript variant 7, (10ug)

Product Images

Circular map for MG225067

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trpm3
• Synonyms: 6330504P12Rik; 9330180E14; AU018608; B930001P07Rik; LTRPC3; MLSN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225067 representing NM_001035244
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGTCCGAGTATCTTTTGATACGAAACCTGATCTCTTGCTACACCTGATGACCAAGGAATGGCAAC
  TGGAGCTTCCCAAACTTCTCATCTCCGTGCACGGAGGGCTGCAGAACTTTGAACTCCAGCCCAAACTCAA
  GCAAGTCTTCGGGAAGGGGCTCATCAAAGCAGCCATGACAACTGGAGCCTGGATCTTCACTGGAGGGGTC
  AACACAGGTGTCATTCGTCATGTTGGAGATGCCTTGAAAGACCATGCATCTAAGTCTCGAGGGAAGATAT
  GTACCATAGGTATCGCCCCCTGGGGAATTGTGGAAAACCAGGAAGACCTGATTGGAAGAGATGTAGTCCG
  ACCATACCAGACCATGTCCAATCCTATGAGCAAGCTCACCGTTCTCAATAGCATGCACTCCCACTTCATC
  CTGGCTGACAATGGGACCACGGGGAAATACGGAGCAGAGGTGAAACTCCGCAGACAACTGGAAAAGCACA
  TTTCACTCCAGAAGATAAACACAAGATGCCTGCCGTTTTTCTCTCTTGACTCCCGCTTGTTTTATTCATT
  TTGGGGTAGTTGCCAATTAGACCCAATTGGAATCGGTCAAGGGGTTCCAGTGGTGGCTCTCATCGTGGAA
  GGAGGACCCAATGTGATCTCAATTGTTTTGGAATACCTTCGAGACACCCCTCCTGTACCAGTCGTGGTCT
  GTGATGGGAGTGGACGGGCATCCGACATCCTGGCATTTGGGCATAAATATTCAGAAGAAGGCGGACTTAT
  CAATGAATCTTTGAGGGACCAGCTGTTGGTGACAATACAGAAGACCTTCACGTACACTCGAACCCAAGCT
  CAGCACCTGTTCATCATCCTCATGGAATGCATGAAGAAGAAGGAACTGATCACAGTGTTTCGAATGGGGT
  CAGAAGGCCACCAGGACATTGATTTAGCTATCCTGACAGCATTACTCAAAGGTGCCAATGCATCCGCCCC
  AGACCAACTGAGCTTAGCTTTAGCCTGGAACAGAGTTGACATCGCTCGCAGCCAGATCTTTATTTACGGG
  CAGCAGTGGCCGGTAGGGTCCCTGGAGCAAGCCATGCTGGATGCCCTAGTCCTGGACAGAGTGGATTTTG
  TGAAATTACTCATAGAAAATGGAAGACATGGGCCCTCAAATACATTGTACCACTTGGTCAGGGATGTCAA
  AAAGGGAAACCTGCCCCCGGACTACAGAATCAGCCTGATAGACATTGGCCTGGTGATCGAGTACCTGATG
  GGCGGGGCTTACCGCTGCAACTACACGCGCAAGCGCTTTCGGACCCTCTACCACAATCTCTTTGGCCCCA
  AAAGGGATGATATTCCCTTGAGGAGAGGACGGAAGACAACTAAGAAGCGTGAGGAAGAGGTGGACATCGA
  CTTGGATGATCCTGAGATCAACCACTTTCCATTCCCCTTCCATGAGCTGATGGTGTGGGCCGTCCTCATG
  AAGAGACAAAAGATGGCCCTGTTCTTCTGGCAACACGGAGAGGAGGCCATGGCCAAAGCCCTGGTGGCCT
  GCAAGCTCTGCAAGGCCATGGCTCACGAGGCTTCTGAGAATGACATGGTCGATGACATTTCCCAGGAGCT
  GAACCACAACTCCAGGGACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAACAGGATGAG
  CAGCTGGCCATGAAGCTGCTGACGTATGAGCTGAAGAACTGGAGTAATGCCACATGCCTGCAGCTGGCTG
  TGGCTGCCAAGCACCGTGACTTCATTGCACACACGTGCAGCCAGATGTTACTTACTGACATGTGGATGGG
  ACGGCTCCGGATGAGAAAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTC
  AGCTTGGAGTTCAAGAACAAAGATGACATGCCCTATATGACTCAGGCCCAGGAGATTCATCTCCAAGAGA
  AGGAGCCGGAGGAGCCAGAGAAGCCCACAAAGGAAAAAGATGAAGAGGACATGGAGCTAACAGCAATGTT
  GGGGCGAAGCAATGGGGAATCATCCAGAAAGAAAGATGAAGAAGAAGTTCAAAGCAGGCACCGGCTAATC
  CCCGTGGGCCGAAAAATCTATGAGTTCTACAACGCACCCATCGTGAAGTTCTGGTTCTACACTCTGGCGT
  ATATTGGATACCTGATGCTCTTCAACTACATCGTGTTAGTGAAGATGGAGCGCTGGCCTTCCACTCAGGA
  ATGGATTGTCATCTCCTACATTTTCACTCTGGGAATAGAGAAGATGAGAGAGATCCTGATGTCGGAGCCG
  GGCAAGCTGCTGCAGAAGGTGAAGGTGTGGCTTCAGGAGTACTGGAACGTCACAGACCTCATCGCCATCC
  TTCTCTTCTCGGTGGGAATGATCCTTCGTCTTCAAGATCAGCCCTTCAGGAGTGACGGGAGGGTCATCTA
  CTGTGTGAACATCATTTATTGGTATATCCGTTTGCTGGACATCTTCGGCGTGAACAAGTATCTGGGCCCA
  TATGTGATGATGATTGGGAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTGCTGA
  TGAGCTTTGGGGTCGCCAGGCAAGCCATTCTCTTTCCCAATGAGGAGCCATCTTGGAAACTGGCCAAGAA
  TATCTTCTACATGCCTTATTGGATGATTTATGGGGAAGTGTTTGCTGACCAGATAGACCGTAAGCAAGTT
  TATGATTCTCATACACCAAAGTCAGGTATTTTAACTTCAATTGAGTCACGTCTTTTCCAAGTCCATTTGT
  TTGTCCTCAATAAATGGCTCAAGATAGAGGACCGATTCCTAGTTTGCTATCTTGGTGGAGACAATCACTG
  CAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225067 representing NM_001035244
  Red=Cloning site Green=Tags(s)
  
  MYVRVSFDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGV
  NTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLIGRDVVRPYQTMSNPMSKLTVLNSMHSHFI
  LADNGTTGKYGAEVKLRRQLEKHISLQKINTRCLPFFSLDSRLFYSFWGSCQLDPIGIGQGVPVVALIVE
  GGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGLINESLRDQLLVTIQKTFTYTRTQA
  QHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYG
  QQWPVGSLEQAMLDALVLDRVDFVKLLIENGRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLM
  GGAYRCNYTRKRFRTLYHNLFGPKRDDIPLRRGRKTTKKREEEVDIDLDDPEINHFPFPFHELMVWAVLM
  KRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNHNSRDFGQLAVELLDQSYKQDE
  QLAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGILLPPSIL
  SLEFKNKDDMPYMTQAQEIHLQEKEPEEPEKPTKEKDEEDMELTAMLGRSNGESSRKKDEEEVQSRHRLI
  PVGRKIYEFYNAPIVKFWFYTLAYIGYLMLFNYIVLVKMERWPSTQEWIVISYIFTLGIEKMREILMSEP
  GKLLQKVKVWLQEYWNVTDLIAILLFSVGMILRLQDQPFRSDGRVIYCVNIIYWYIRLLDIFGVNKYLGP
  YVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFADQIDRKQV
  YDSHTPKSGILTSIESRLFQVHLFVLNKWLKIEDRFLVCYLGGDNHCR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035244
• ORF Size: 2874 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035244.2
• RefSeq Size: 3653 bp
• RefSeq ORF: 2877 bp
• Locus ID: 226025
• Cytogenetics: 19 B