Adarb1 (NM_130895) Mouse Tagged ORF Clone

CAT#: MG225001

Adarb1 (tGFP-tagged) - Mouse adenosine deaminase RNA-specific B1 (Adarb1) transcript variant 1, (10ug)

Product Images

Circular map for MG225001

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adarb1
• Synonyms: 1700057H01Rik; AD; Adar2; AW124433; AW558573; BB220382; D10Bwg0447e; RED; Red1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225001 representing NM_130895
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATAGAAGATGAAGAGAATATGAGTTCCAGCAGCACTGATATTAAAGAAAACCGCAATCTGGACA
  ACATGCCCCCCAAGGACAGCAGCACACCTGGGCCTGGCGAGGGTATTCCGCTCTCCAACGGGGGTGGTGG
  TAGCACCAGCAGGAAGCGGCCCCTGGAGGAGGGCAGCAATGGTCACTCCAAGTACCGCCTGAAGAAGCGA
  AGGAAAACACCAGGGCCTGTTCTGCCCAAGAACGCCCTGATGCAGCTGAACGAGATCAAACCTGGCTTAC
  AGTACATGCTGCTGTCCCAGACAGGACCCGTGCATGCACCTCTGTTTGTCATGTCTGTGGAGGTAAACGG
  GCAGGTTTTTGAGGGCTCTGGCCCTACAAAGAAAAAGGCAAAACTCCATGCTGCTGAGAAGGCCCTGAGG
  TCTTTTGTCCAGTTTCCCAACGCCTCCGAGGCCCACCTAGCCATGGGAAGGACCCTCTCTGTGAACACAG
  ACTTCACGTCTGACCAGGCTGACTTCCCTGACACACTCTTCAATGGCTTCGAGACTCCAGACAAGTCAGA
  GCCACCCTTCTACGTAGGCTCCAACGGGGATGACTCGTTCAGCTCAAGTGGAGATGTCAGCCTATCGGCC
  TCCCCAGTGCCTGCCAGCCTTACCCAGCCTCCTCTGCCCATCCCACCACCATTCCCACCCCCAAGTGGGA
  AGAATCCCGTGATGATCTTGAATGAGCTACGCCCAGGGCTGAAGTATGACTTCCTCTCTGAGAGTGGGGA
  GAGCCACGCCAAGAGCTTCGTCATGTCCGTGGTGGTAGATGGCCAGTTCTTTGAGGGCTCAGGAAGAAAC
  AAGAAGCTTGCCAAGGCCCGGGCTGCACAGTCTGCCTTGGCTACTGTCTTCAATTTGCACTTGGACCAAA
  CACCATCTCGCCAGCCTGTCCTCAGTGAGGGTCTTCAGTTGCATTTGCCACAGGTATTGGCAGATGCTGT
  CTCCCGCCTGGTCCTGGGTAAGTTCAGTGACCTGACAGACAACTTTTCCTCCCCTCATGCACGAAGGAAA
  GTGCTCTCTGGAGTAGTGATGACCACAGGTACAGATGTCAAAGATGCCAAGGTGATAAGTGTTTCGACAG
  GGACGAAGTGTATCAACGGTGAATACATGAGTGACCGTGGCCTCGCACTCAATGACTGCCACGCAGAGAT
  AATCTCCCGAAGGTCCCTGCTCAGGTTTCTTTATGCACAGCTCGAGCTTTATTTAAATAACAAAGAAGAC
  CAGAAAAAGTCCATATTTCAGAAGTCAGAGCGGGGTGGGTTCCGGCTGAAGGATACCGTGCAGTTCCACC
  TGTACATCAGCACCTCGCCCTGCGGAGACGCCAGAATATTCTCTCCCCACGAGCCCGTGCTAGAGGAACC
  AGCAGATAGACATCCGAATCGCAAAGCAAGGGGACAGCTACGGACGAAAATAGAGTCTGGCGAGGGGACA
  ATCCCTGTGCGCTCAAATGCCAGCATCCAGACCTGGGACGGGGTGCTGCAGGGGGAACGGCTGCTCACCA
  TGTCCTGCAGTGACAAGATAGCACGCTGGAACGTGGTGGGCATCCAGGGGTCCCTGCTCAGCATTTTCGT
  GGAACCCATCTACTTCTCCAGCATCATCTTGGGCAGCCTGTACCACGGGGACCACCTCTCCAGGGCCATG
  TACCAGCGGATCTCCAACATAGAGGACCTGCCACCGCTCTACACTCTCAACAAGCCCCTGCTCAGCGGCA
  TCAGCAATGCAGAGGCACGGCAGCCAGGGAAGGCACCCAACTTCAGTGTCAACTGGACAGTGGGTGACGC
  CACCATTGAGGTCATCAATGCCACAACAGGGAAGGATGAGCTTGGCCGCCCATCCCGCCTGTGTAAGCAC
  GCGCTGTACTGTCGCTGGATGCGTGTACACGGCAAGGTTCCCCCCCACCTGCTGCGCACCAAGATCACTA
  AGCCTACCACATACCACGAGTCCAAGCTGGCAGCGAGGGAGTACCAGGCTGCCAAGGCCCGTCTGTTCAC
  TGCCTTCATCAAGGCGGGGCTGGGCGCCTGGGTGGAGAAGCCCACAGAGCAGGACCAGTTCTCCTTCACT
  CCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225001 representing NM_130895
  Red=Cloning site Green=Tags(s)
  
  MDIEDEENMSSSSTDIKENRNLDNMPPKDSSTPGPGEGIPLSNGGGGSTSRKRPLEEGSNGHSKYRLKKR
  RKTPGPVLPKNALMQLNEIKPGLQYMLLSQTGPVHAPLFVMSVEVNGQVFEGSGPTKKKAKLHAAEKALR
  SFVQFPNASEAHLAMGRTLSVNTDFTSDQADFPDTLFNGFETPDKSEPPFYVGSNGDDSFSSSGDVSLSA
  SPVPASLTQPPLPIPPPFPPPSGKNPVMILNELRPGLKYDFLSESGESHAKSFVMSVVVDGQFFEGSGRN
  KKLAKARAAQSALATVFNLHLDQTPSRQPVLSEGLQLHLPQVLADAVSRLVLGKFSDLTDNFSSPHARRK
  VLSGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYAQLELYLNNKED
  QKKSIFQKSERGGFRLKDTVQFHLYISTSPCGDARIFSPHEPVLEEPADRHPNRKARGQLRTKIESGEGT
  IPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFVEPIYFSSIILGSLYHGDHLSRAM
  YQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPNFSVNWTVGDATIEVINATTGKDELGRPSRLCKH
  ALYCRWMRVHGKVPPHLLRTKITKPTTYHESKLAAREYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSFT
  P
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130895
• ORF Size: 2103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130895.3, NP_570965.2
• RefSeq Size: 6572 bp
• RefSeq ORF: 2106 bp
• Locus ID: 110532
• UniProt ID: Q91ZS8
• Cytogenetics: 10 39.72 cM
• Gene Summary: This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site, alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]