Uspl1 (NM_001115149) Mouse Tagged ORF Clone

CAT#: MG224848

Uspl1 (tGFP-tagged) - Mouse ubiquitin specific peptidase like 1 (Uspl1) transcript variant 2, (10ug)

Product Images

Circular map for MG224848

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Uspl1
• Synonyms: E430026A01Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224848 representing NM_001115149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGATGATTCTCTGAAGATTGGAAATGGTTTGCCACTGGTTGGACCAGGGACTGATGTAGGGATAT
  CTTCACTCCCCATGCTGGGGTACTTGGGAAAAAATTATGCTTCAGCTAAAGTCACAACAGACGGGCATTG
  CCCTGCTTGTAGAGCGAAAGGCAAGCTAAGTGCCTTAAAACCTTACCGCATTAGCTTCCAAGAATCTGTC
  TTTCTGTGTGAGGATCTTCAGTGCATTTATCCTTTGGGCTCTGAGTCACTTACTAATTTAATTTCTCCTG
  ATTCAGAAGATTGTCCCACTCCAAGTAAGCCTCAAAAACGAAAAAGATTGGAAACCAACTGTAGGAATTC
  ACCTCTTCCAGTACATTCTAAAAAGACTAGAAGTCACATTGTCACTGATAGTGAACCAATTGTGAATGGT
  AAATATAATGGAGAAGTGTGTGATGACTTCTCAGCAAGCTTCCCAGATACCAGTGCTCACCAGGATCCTG
  CCAGCACAGCTGCTTCTGTGGAGCAGAGTGAGGCTTTGGAGGCTGATGATGTGGTTGTGGCCGCTACAGA
  AGATCCTGCTACAGTTAGTGTCACATCTGAGCTGGAAATGCCAGCTAAGAGCAGATGTTTACCACTTTGC
  CAGACTCTGTGTGTCCAGTGGAAAAACACCCAGGCCCTGTGCTGGTTAGACTGTATCCTGTCAGCCCTGG
  TGCACTTGGAGGTGTTGAGGAAGACTGTGCTGGAGGCGTGCTCCAGGGAGGAGTGTGTATTTGGAAGGCT
  GTTTGAAATGTACCACCAAGCAGATGAGCTGCTGCACACCCATCACCTGCATGGCGTCACAGGTGAAGAT
  TGTAAAAAATTGACATCAGAAATATTTACAGAAATAGATACCTGTTTGAATAAAGTTAGAGATGAAATTT
  TTGCTAAACTTCAACCGAAGCTTAGATGCACATTAGGTGACATGGAAAGTCCTGTGTTTGCACTTCCTGT
  ACTGTTAAAGCTTGAACCCCATGTTGAAAGCCTCTTTACATATTCTTTTTCTTGGAATTTTGAATGTTCC
  CATTGTGGACACCAGTACCAAAACAGGTGTGTGAAGAGTCTGGTCACCTTTACCAATATTGTTCCTGAGT
  GGCATCCACTCAATGCTGCCCATTTTGGTCCATGTAACAGCTGCAACAGTAAATCACAAATAAGAAAAAT
  GGTGTTGGAAAGAGCGTCGCCCATACTCATGCTGCACTTCGTGGAGGGCTTACCAAGGAGGGATTTGCAA
  CACTATGCCTTCCACTTTGAAGGAAGCCTTTATCAAGTCACGTCGGTAATACAGTACCAAGCAAATAACC
  ATTTTATAACATGGTTTTTAGATGCTGATGGGAGTTGGCTGGAGTGTGATGACCTAAAGGGTCCCTGTGC
  TAAAAGGCACGTGACCTGTGAAGTGCCAGCTTCAGAGACACATATCGTTATTTGGGAAAGAAAATCCCAG
  GTGCCAATTGAAGAAGCTGCCTGCCTTCCATGCATGAAGCCAAATGTGCAGCCTGTATCAGGTGAGGAGC
  AGCCGACTTGTCCAGCACTGTGTTCTCTGGCTGGGACCGCCACCTCAGAACCCTCAGTCGCCCATCCCAC
  ATCTATGGCAGGCGCTCCTCAGACTCTTCCAGAAATCCAAGCTGTAGCTCATGGAGATAGTGTACTTTCA
  GGAGCAAAAGGCATGGTCGACAGCATTTTACCCTCAGCACTTGAAGAAACCATCCAGGAAACTGCCTCTG
  TTTCTCAGGTTGACTCCAAAGATTGCCTGTTGGAAGACAAACCCGTGGCAGGAAGTGCAGCACTGGTCAG
  AGTACTTGCTTTTCAGCCACAGGACTCCCCGGGGTCTTCGGGGTCTTCCTTAGTGTCCAGCCTGTGTGAA
  GGAAAGCTAGTTGCCCCATGTGTGGATTCAAGTTTCCCATCCCAAGCTGTGAGCACAGACCTGCAGGCAG
  TACTGTCCCAGGCAGGGGACACTGTGGTTCCTAACCCTGTGACTGATGCCCCTGTTCCTGTTCTTGTTCA
  GGAATTGAAGTCCCTGGCAACTGAGAAGGACTCTCAGACACAGTTGCTGCCACTGAAAACTGAGAAGTTA
  GATCCTGAGCAACCTGGTAAATCTCAGGCATCTAATTTGAGAAAACGAGAAACCACAGCGTCTTCTAAAA
  CAGTAGCAGCCAGGTCAGCACAGAATCAGCCTCGGAAAGAAGATCAGAAGAGAGCCTTCGTGGGAAGTTG
  GGTGAAGGGGCTATTGAGCAGGGGTGGTGCTTTTATGCCAACCTGTGTTTTGTCTCAGAGTAGAGCAGTA
  AGTGACCTGCAACCTTCTGTTAAAGGAGCCAGTAATTTTGATGGCTTTAAAACGAAAAGTATAAGCCGCA
  GGTCTAAAAGAATGTCCAGGAAAGCTAAGCACATGGAGGAACTGTCTCCAAGGAACAGCTCTCCACCATT
  GAGCTGGACAGCTGCTCTGACCCAGGCTGCTGAGAACGCCACCTCAGCTCTGTTGAGGGAGCAGGAAGGC
  TCACGTCCAGCTCCCCTCAGGCACAGATCTCCTGGCAATGAAAGCGCCATCTCTCCAGCAAGCCGTGGAG
  ATGCGGCTGAAGATCAGGTTCATAAGCTTCGGCTCAAACTTCTTAAAAAATTAAAGGCAAAAAAGAAGAA
  ACTAGCTGCCCTCATTTCTTCCCCGCACCGTGAACCATCTCTAAGTGACCACTCAGAGCCTGCGTCCCAT
  TGTGGAACACCTGCAAGTGACCAGTCAGAGCCTGTATCCCATTGTGGATCTCCAAATGACTGTGAGTCCA
  TAGAAGATCTGTTAAAAGAGCTACAGCATCAAATTGACCTTGCAGACAGCAAATCTGGGTGCACCTCTGC
  TCCTGATGCCACCTCAAATAACAGCCAGAGTCATGAGGAGATTTTAGCAGAGTTGCTGTCGCCTACAGCC
  ATGTCAGAGCCCTCAGAAAGTGGGGAGCTTGAGTTGAGGTACTTGGAAATGGGGGACAGCACCCCAGCAC
  AAGCACCTAGTGAATTCAGTGTTGTCTCCCAGAACACATGTCTGAAACAGGACCATGATTACTGCAGCCC
  TGAGAAAGGCCAGCGGGAAGTTGATCTGCATTCAGTCATGGACAGTGCCTGTATAAGAACCTTGAACTTG
  GGGAGTCCCATGAAGACTGATATCTTTGATGATTTTTTTTCCACCTCAGCACTGAATTCTTTAACAAACG
  ACACATTAGACATACCTCATTTTGATGACTCTCTGTTTGAGAATTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224848 representing NM_001115149
  Red=Cloning site Green=Tags(s)
  
  MTDDSLKIGNGLPLVGPGTDVGISSLPMLGYLGKNYASAKVTTDGHCPACRAKGKLSALKPYRISFQESV
  FLCEDLQCIYPLGSESLTNLISPDSEDCPTPSKPQKRKRLETNCRNSPLPVHSKKTRSHIVTDSEPIVNG
  KYNGEVCDDFSASFPDTSAHQDPASTAASVEQSEALEADDVVVAATEDPATVSVTSELEMPAKSRCLPLC
  QTLCVQWKNTQALCWLDCILSALVHLEVLRKTVLEACSREECVFGRLFEMYHQADELLHTHHLHGVTGED
  CKKLTSEIFTEIDTCLNKVRDEIFAKLQPKLRCTLGDMESPVFALPVLLKLEPHVESLFTYSFSWNFECS
  HCGHQYQNRCVKSLVTFTNIVPEWHPLNAAHFGPCNSCNSKSQIRKMVLERASPILMLHFVEGLPRRDLQ
  HYAFHFEGSLYQVTSVIQYQANNHFITWFLDADGSWLECDDLKGPCAKRHVTCEVPASETHIVIWERKSQ
  VPIEEAACLPCMKPNVQPVSGEEQPTCPALCSLAGTATSEPSVAHPTSMAGAPQTLPEIQAVAHGDSVLS
  GAKGMVDSILPSALEETIQETASVSQVDSKDCLLEDKPVAGSAALVRVLAFQPQDSPGSSGSSLVSSLCE
  GKLVAPCVDSSFPSQAVSTDLQAVLSQAGDTVVPNPVTDAPVPVLVQELKSLATEKDSQTQLLPLKTEKL
  DPEQPGKSQASNLRKRETTASSKTVAARSAQNQPRKEDQKRAFVGSWVKGLLSRGGAFMPTCVLSQSRAV
  SDLQPSVKGASNFDGFKTKSISRRSKRMSRKAKHMEELSPRNSSPPLSWTAALTQAAENATSALLREQEG
  SRPAPLRHRSPGNESAISPASRGDAAEDQVHKLRLKLLKKLKAKKKKLAALISSPHREPSLSDHSEPASH
  CGTPASDQSEPVSHCGSPNDCESIEDLLKELQHQIDLADSKSGCTSAPDATSNNSQSHEEILAELLSPTA
  MSEPSESGELELRYLEMGDSTPAQAPSEFSVVSQNTCLKQDHDYCSPEKGQREVDLHSVMDSACIRTLNL
  GSPMKTDIFDDFFSTSALNSLTNDTLDIPHFDDSLFENC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001115149
• ORF Size: 3267 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115149.1, NP_001108621.1
• RefSeq Size: 3772 bp
• RefSeq ORF: 3270 bp
• Locus ID: 231915
• UniProt ID: Q3ULM6
• Cytogenetics: 5 G3
• Gene Summary: SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms (By similarity). Plays a key role in RNA polymerase-II-mediated snRNA transcription in the Cajal bodies (By similarity). Is a component of complexes that can bind to U snRNA genes (By similarity).[UniProtKB/Swiss-Prot Function]