Bbs9 (NM_178415) Mouse Tagged ORF Clone

CAT#: MG224515

Bbs9 (tGFP-tagged) - Mouse Bardet-Biedl syndrome 9 (human) (Bbs9) transcript variant 1, (10ug)

Product Images

Circular map for MG224515

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bbs9
• Synonyms: E130103I17Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224515 representing NM_178415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTATTTAAAGCCCGTGACTGGTGGTCTACTGTGTTGGGAGAAAAAGAAGAATTTGACCAGGGCT
  GCTTATGCTTGGCTGATGTGGACAACAGTGGGAACGGCCACGATAAAATCATTGTGGGCAGCTTCATGGG
  CTACCTGAGAATCTTTAGTCCCCATTCTGTAAAAGCTGGAGGTGGGCCTCAGGCTGAAGATTTACTTCTA
  GAAGTGCATCTGCGGGACCCAGTGCTGCAAGTGGAAGTCGGGAAGTTTGTCTCGGGCACCGAGATGCTTC
  ATCTGGCCGTGCTGCACTCCCGGAAGCTCTGTGTCTACTCCGTCTCAGGGACATTGGGAAATGTGGAACA
  TGGAAACCAATATCAGATAAAACTAATGTATGAGCACCATCTTCAGAGGACGGCCTGCAATATGACGTAT
  GGACCCTTTGGTGGTGTAAAAGGTCGGGATTTAATTTGTATCCAGTCTCTGGATGGGATGCTGATGGTGT
  TTGAGCAGGAAAGCTATGCGTTTGGGAGGTTTCTCCCTGGTTTTCTTCTGCCTGGCCCTCTTGCTTACAG
  TCCCCGCACGGATTCCTTCATAACTGTGTCTTCTTGCAGACAAGTGGAAAGTTACAAGTACCAGGTACTT
  GCCTTTGCCACAGATGCAGACAAGAAGCAGGAGATGGAACAGCAAAAACTTGGTTCTGGAAAGCGGCTAG
  TTGTGGACTGGACTCTGAATATTGGCGAGCAAGCCCTTGATATCTGTATCTTCCCTTTGAACCAATCTGC
  ATCTTCTGTTTTTGTTCTTGGTGAGAGAAACTTCTTTTGCCTAAAGGATAATGGGCAGATTCGATTCATG
  AAGAAGCTCGGTTATAACCCCAGTTGTTTTCTACCGTACTGCTCAGTTTCTGAAGGTACAATAAATACTT
  TGATTGGAAACCATAACCACATGCTGCATATTTACCAGGACGTGACACTGAAGTGGGCCACACAACTCCC
  CCACGTCCCCGTGGCCGTCAGAGTAGGCTGTTTTCATGATTTGAAGGGAGTGATAGTGACTCTCAGTGAT
  GACGGTCACCTGCAGTGCTCGTATCTGGGGACAGACCCTTCTTTGTTCCAGGCTCCAAAAGTTGAATCAA
  GAGAGCTCAACTATGATGAACTTGATGTGGAATTGAAAGAACTTCAGAAAATCATCAAAGATGTAAAGTT
  GCAAGGTGTTTGGCCCTTGACTGAACAGGAAGATGACTTGAAAGTCTCTGCTTCAGTGTCTTCTACCCTG
  GATTCTGTGTCTCAAGCCACCAATGTGGAACCTGGAGCTGACTCTGTCCCGTCCATCACAGTGAAGATTA
  CCCTGCAGAACCGAGTGGTGTTGCAGAAGGTCAAGCTGTCTATCTATGTGCAGCCTCCACTACAGTTGAC
  TTGTGATCAGTTCACCTTTGACTTCACGGTTCCAGATATGACGAGTTCAGTAGCGTTTTCTGTGTACCTG
  AAGAGAAACTACACACCATCCGAGTTAGAAGGAAATGCTGTTGTTTCTTACTCCAGACCGACAGGTATTC
  CTCGGGTTGTCCAATGTAAATTTAGACTTCCTCTGAAACTGATTTGCCTACCTGGTCAGCCTTCAAAAAC
  TGCAAGCCACAAACTAACTATAGACACCAACAAATCTCCTGTCAGTCTGCTCGGTCTCTTCCCAGACTTT
  GCCAACCCATCAGATGACGACCAAGTGAATGTAATGGGCTTCCGTCTCCTAGGAGGCGCTCGAGTGACTC
  TTCTTGCTTCCAGAACATCCCAACGATACCGCATTCAGAGCGAACAGTTTGAAGATCTCTGGCTCATAAC
  CAATGAACTCATCCTCCGCCTTCAGGAACATTTTGAAAAACAGGGAACCAAAGACTTTTCGTGTTCTTTT
  TCTGGGTGTGTGCCCCTTCAAGAATATTTTGAGTTGATTGATCATCATTTTGAGCTGCGGATAAATGGTA
  AAAAATTGGAAGAACTTTTATCTGAAAGAGCTGTACAATTTCGGGCCATTCAGCGTCGGCTTCTGACAAG
  ATTCAGAGACAAGACCCCTGCCCCACTGCAGCACCTGGACACCCTGCTGGACGGGACATACAAGCAGGTG
  ATTGCTCTAGCAGATGCGATCGAGGAGAACCAGGACAGGCTGTTGCAGTCATTCTCAGGCCTGAAGAGTG
  CCACCCATTTGCTGATCCTGCTAATCAGGCTGTGGCAGAGGCTGAGCGCTGACCAGACTGCTATTCTGGA
  AGCAGCATTTCTGCCACTACAGGAAGACACGCAGGAACTGGGCTGGGAAGAAACCGTGGATGCTGCCATT
  GCCTACCTTTTGAAAACCTGCCTGTCGAAGAGTTCCAAGGAGCAGGCTTTGAACCTCAGCAGCCAACTGA
  ACATACCCAAAGATACCAGCCGGCTGAAGAAGCACATCACCCTGCTGTGTGATAGGTTAGCCAAAGGTGG
  GCGTCTCTGCGTGAGCACAGATGCAGCGGCCCCGCAGGCCATGGTTGTGCCAGGTGGCTGTACTCCAATC
  CCAGAGTCAGACCTAGAGGAAAGGTCACTAGATGACTCCACAGAGCTGTTTACCAACCACAAGCACCTCA
  TGACCGAGCCCCCCATGCCTGAAGTCTCAGCCCGCCAAGGAGTTTTGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224515 representing NM_178415
  Red=Cloning site Green=Tags(s)
  
  MSLFKARDWWSTVLGEKEEFDQGCLCLADVDNSGNGHDKIIVGSFMGYLRIFSPHSVKAGGGPQAEDLLL
  EVHLRDPVLQVEVGKFVSGTEMLHLAVLHSRKLCVYSVSGTLGNVEHGNQYQIKLMYEHHLQRTACNMTY
  GPFGGVKGRDLICIQSLDGMLMVFEQESYAFGRFLPGFLLPGPLAYSPRTDSFITVSSCRQVESYKYQVL
  AFATDADKKQEMEQQKLGSGKRLVVDWTLNIGEQALDICIFPLNQSASSVFVLGERNFFCLKDNGQIRFM
  KKLGYNPSCFLPYCSVSEGTINTLIGNHNHMLHIYQDVTLKWATQLPHVPVAVRVGCFHDLKGVIVTLSD
  DGHLQCSYLGTDPSLFQAPKVESRELNYDELDVELKELQKIIKDVKLQGVWPLTEQEDDLKVSASVSSTL
  DSVSQATNVEPGADSVPSITVKITLQNRVVLQKVKLSIYVQPPLQLTCDQFTFDFTVPDMTSSVAFSVYL
  KRNYTPSELEGNAVVSYSRPTGIPRVVQCKFRLPLKLICLPGQPSKTASHKLTIDTNKSPVSLLGLFPDF
  ANPSDDDQVNVMGFRLLGGARVTLLASRTSQRYRIQSEQFEDLWLITNELILRLQEHFEKQGTKDFSCSF
  SGCVPLQEYFELIDHHFELRINGKKLEELLSERAVQFRAIQRRLLTRFRDKTPAPLQHLDTLLDGTYKQV
  IALADAIEENQDRLLQSFSGLKSATHLLILLIRLWQRLSADQTAILEAAFLPLQEDTQELGWEETVDAAI
  AYLLKTCLSKSSKEQALNLSSQLNIPKDTSRLKKHITLLCDRLAKGGRLCVSTDAAAPQAMVVPGGCTPI
  PESDLEERSLDDSTELFTNHKHLMTEPPMPEVSARQGVLE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178415
• ORF Size: 2640 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178415.1, NP_848502.1
• RefSeq Size: 3438 bp
• RefSeq ORF: 2643 bp
• Locus ID: 319845
• UniProt ID: Q811G0
• Cytogenetics: 9 A3
• Gene Summary: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Required for proper BBSome complex assembly and its ciliary localization (By similarity).[UniProtKB/Swiss-Prot Function]