Abcc3 (NM_029600) Mouse Tagged ORF Clone

CAT#: MG224428

Abcc3 (tGFP-tagged) - Mouse ATP-binding cassette sub-family C (CFTR/MRP) member 3 (Abcc3), (10ug)

Product Images

Circular map for MG224428

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abcc3
• Synonyms: 1700019L09Rik; ABC31; MLP2; MOAT-D; MRP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224428 representing NM_029600
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGCCTGTGCGGCTCCGGAGAGCTGGGCTCCAAGTTCTGGGACTCCAACCTGTCTATATATACCA
  ACACTCCGGACCTCACACCCTGTTTCCAGAACTCCTTGCTGGCCTGGGTCCCCTGCATCTACCTGTGGGC
  TGCCTTGCCCTGCTACCTGTTTTACCTGAGACACCATCAGCTCGGCTACATAGTCCTCTCATGGTTATCC
  AGGCTCAAGACGGCCCTCGGTGTTCTGCTGTGGTGTGTCTCATGGGTGGACCTGTTCTATTCCTTCCACG
  GCCTGATCCATGGCTCATCCCCTGCTCCTGTCTTCTTTGTCACACCCTTGGTGGTGGGGATCACCATGCT
  GCTGGCCACTTTGCTAATCCAGTACGAGCGGCTTCGGGGGGTGCAGTCTTCGGGAGTGCTCATCATATTC
  TGGCTCCTGTGTGTGATCTGTGCCATCATCCCCTTCCGTTCCAAGATCCTCTCAGCTTTGGCAGAGGGTA
  AAATCTTGGATCCGTTCCGGTTCACCACTTTCTACATCTACTTTGCCCTGGTGTTCTGTGCCCTCATCCT
  GTCCTGCTTCAAGGAGAAGCCGCCTTTGTTCTCCCCGGAGAATCTTGACACAAATCCTTGCCCAGAGGCC
  AGCGCTGGCTTCTTTTCCCGCTTGTCTTTCTGGTGGTTCACAAGGCTTGCCATCCTTGGCTACCGACGTC
  CCCTGGAGGACCGTGACCTCTGGTCTCTGTCTGAGGAGGACTGCTCTCACAAGGTGGTACAACGGCTGCT
  GGAAGCATGGCAAAAGCAGCAGAACCAAGCATCAGGGTCCCAGACGGCAACAGCTGAGCCAAAGATCCCA
  GGTGAGGATGCGGTCCTACTGAAGCCCCGACCCAAGTCCAAGCAGCCTTCCTTTCTGAGGGCTTTGGTGA
  GAACCTTCACCTCCAGCTTGCTCATGAGTGCCTGCTTCAACCTGATCCAGAACCTACTCGGCTTCGTCAA
  CCCACAGCTGCTCAGTATACTCATCAGGTTTATTTCTGACCCCACGGCCCCCACCTGGTGGGGCTTCTTG
  CTGGCCGGACTGATGTTCCTGAGCTCCACCATGCAGACATTGATCTTACACCAATATTACCACTGCATCT
  TTGTGATGGCCTTGAGGCTACGGACTGCTATTATAGGTGTCATCTACAGGAAGGCTCTGGTCATCACCAA
  CTCAGTCAAACGGGAGTCCACTGTGGGAGAAATGGTCAACCTCATGTCAGTGGATGCTCAGCGCTTCATG
  GATGTCTCCCCTTTCATCAATCTGCTGTGGTCTGCACCTTTGCAGGTCATCCTGGCAATATACTTCCTCT
  GGCAGATCCTGGGCCCATCAGCTCTGGCTGGAGTGGCTGTGATAGTCTTGCTGATACCACTCAATGGAGC
  TGTGTCCATGAAGATGAAGACCTACCAGGTAAAGCAAATGAAGTTCAAGGACTCCCGCATCAAGCTGATG
  AGCGAGATCCTGAACGGCATCAAAGTGCTCAAGCTGTACGCTTGGGAGCCCAGCTTCTTGGAGCAGGTGA
  AAGGGATCAGGCAGAGTGAACTTCAGCTGCTCCGGAAGGGGGCCTACCTGCAGGCCATCTCCACCTTCAT
  CTGGATCTGCACCCCATTTCTGGTGACCCTGATCACCCTCGGAGTGTACGTGTATGTGGACGAGAGCAAT
  GTACTGGATGCCGAGAAGGCCTTTGTGTCCCTGTCTTTGTTCAATATCTTAAAGATCCCCCTCAACATGC
  TGCCTCAGTTAATCAGTGGACTGACCCAGGCCAGCGTGTCTCTGAAACGGATCCAGGATTTCCTGAACCA
  AAATGAACTTGACCCCCAATGTGTGGAAAGAAAGACCATCTCCCCAGGCTACGCCATCACCATACACAAC
  GGCACCTTCACCTGGGCCCAGGACCTGCCGCCCACCCTGCACAGCCTAAACATTCAAATCCCGAAGGGGG
  CGTTGGTGGCTGTGGTGGGACCTGTGGGCTGTGGGAAGTCTTCTCTGGTGTCTGCCTTACTCGGAGAGAT
  GGAGAAGCTGGAAGGTGTGGTGTCTGTAAAGGGCTCTGTGGCCTACGTGCCCCAGCAGGCATGGATCCAG
  AACTGCACACTTCAGGAAAATGTGCTATTTGGCCAACCCATGAACCCCAAGCGCTACCAACAGGCTCTGG
  AGACATGTGCCCTGCTAGCTGACCTAGATGTGCTTCCTGGTGGGGACCAGACAGAGATTGGAGAGAAGGG
  TATTAACCTATCTGGAGGCCAGCGCCAACGGGTGAGTTTGGCCCGAGCTGTTTATAGTGATGCCAACATT
  TTTTTGCTGGATGACCCACTGTCGGCTGTGGACTCTCATGTGGCGAAGCATATCTTCGACCAAGTGATTG
  GACCAGAAGGTGTGCTGGCAGGCAAGACTCGGGTGCTGGTAACTCATGGCATCAGCTTCCTGCCCCAGAC
  GGACTTTATCATTGTGCTTGCTGGTGGACAGGTGTCTGAGATGGGCCACTACTCGGCCCTCCTGCAGCAT
  GATGGCTCCTTTGCCAACTTCCTCCGAAACTACGCACCAGATGAAGACCAGGAGGACCATGAAGCAGCCT
  TGCAAAATGCAAATGAGGAGGTACTCCTGCTCGAAGACACACTCAGCACCCACACAGACCTGACAGACAA
  TGAGCCAGCCATCTACGAGGTCCGCAAGCAGTTCATGAGAGAGATGAGCTCCTTGTCTTCTGAAGGGGAG
  GTCCAGAACCGGACTATGCCCAAGAAACACACAAATTCATTGGAGAAGGAGGCGCTGGTGACAAAGACTA
  AGGAGACTGGCGCATTAATCAAAGAGGAGATAGCAGAGACAGGCAATGTGAAGCTGAGTGTGTACTGGGA
  TTATGCCAAGTCTATGGGACTCTGTACCACGCTATCTATCTGCCTCCTGTATGGTGGCCAAAGCGCAGCT
  GCTATCGGAGCCAATGTGTGGCTCAGCGCCTGGAGCAATGATGCGGAGGAACATGGCCAGCAGAACAAGA
  CCTCCGTAAGGCTCGGTGTCTACGCCGCCCTAGGGATACTGCAAGGGCTCCTGGTCATGTTGTCAGCTTT
  CACCATGGTGGTCGGCGCGATCCAGGCTGCCCGCCTGCTGCACGAAGCTCTGCTGCACAACAAGATTCGT
  TCCCCTCAGTCCTTCTTTGACACCACGCCCTCGGGCCGCATCCTCAACCGTTTCTCCAAGGACATATACG
  TCATCGACGAGGTTCTGGCCCCCACCATCCTCATGCTGTTGAATTCCTTCTTCACATCCATCTCCACCAT
  CATGGTCATCGTGGCCAGCACGCCGCTCTTCATGGTGGTTGTCTTGCCCCTGGCTGTGCTCTATGGCTTT
  GTGCAGCGCTTCTATGTGGCCACATCACGGCAGCTCAAGCGGCTGGAATCCATCAGCCGCTCACCCATCT
  TCTCCCACTTTTCGGAGACAGTAACGGGCACCAGTGTCATTCGGGCCTATGGCCGAATCCAAGACTTCAA
  GGTCCTCAGTGATACTAAGGTGGACAACAACCAGAAAAGCAGTTATCCCTACATTGCCTCCAATCGATGG
  CTGGGGGTCCACGTGGAGTTTGTGGGGAACTGCGTGGTGCTCTTTGCCGCACTGTTTGCAGTGATCGGGA
  GAAACAGCTTGAATCCAGGGCTTGTGGGTCTTTCTGTGTCCTATGCCTTACAGGTGACCATGGCTTTGAA
  TTGGATGATACGAATGATATCCGACTTGGAGTCTAATATCATAGCTGTGGAGAGAGTCAAGGAGTACTCT
  AAGACCAAGACTGAGGCCCCCTGGGTGGTGGAGAGCAACCGCGCCCCGGAAGGCTGGCCCACGCGAGGAA
  TGGTGGAGTTCCGGAACTATTCAGTGCGGTACCGGCCTGGCCTCGAGTTGGTGCTGAAGAACGTGACTGT
  TCATGTGCAGGGTGGGGAGAAGGTAGGCATTGTGGGCCGCACTGGGGCTGGCAAATCTTCCATGACTCTT
  TGCCTGTTCCGAATCCTGGAGGCCGCAGAGGGTGAGATCGTCATTGATGGGCTCAACGTGGCACACATTG
  GCCTCCACGACCTGCGCTCTCAGCTCACCATCATCCCTCAGGACCCCATCCTGTTCTCGGGCACCCTGCG
  TATGAACTTAGATCCCTTTGGCCGATACTCGGAGGAGGACATCTGGAGGGCCCTGGAGCTATCCCACCTG
  AACACGTTCGTGAGCAGCCAGCCAGCAGGCCTGGATTTCCAGTGCGCTGAGGGTGGGGATAATCTCAGTG
  TTGGCCAGAGGCAGCTTGTATGCCTAGCCCGAGCCCTGCTCCGGAAGAGCCGAGTCCTGGTTTTAGACGA
  GGCCACTGCTGCCATTGACTTGGAGACGGATGACCTCATCCAGGGCACCATCCGTACCCAGTTTGAAGAC
  TGCACCGTACTGACCATCGCCCACCGGCTCAACACAATCATGGACTACAACAGGGTCCTGGTCTTGGACA
  AAGGAGTAGTAGCTGAATTTGATTCTCCAGTCAACCTCATTGCGGCCGGAGGCATCTTCTATGGGATGGC
  CAAGGATGCGGGACTTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224428 representing NM_029600
  Red=Cloning site Green=Tags(s)
  
  MDRLCGSGELGSKFWDSNLSIYTNTPDLTPCFQNSLLAWVPCIYLWAALPCYLFYLRHHQLGYIVLSWLS
  RLKTALGVLLWCVSWVDLFYSFHGLIHGSSPAPVFFVTPLVVGITMLLATLLIQYERLRGVQSSGVLIIF
  WLLCVICAIIPFRSKILSALAEGKILDPFRFTTFYIYFALVFCALILSCFKEKPPLFSPENLDTNPCPEA
  SAGFFSRLSFWWFTRLAILGYRRPLEDRDLWSLSEEDCSHKVVQRLLEAWQKQQNQASGSQTATAEPKIP
  GEDAVLLKPRPKSKQPSFLRALVRTFTSSLLMSACFNLIQNLLGFVNPQLLSILIRFISDPTAPTWWGFL
  LAGLMFLSSTMQTLILHQYYHCIFVMALRLRTAIIGVIYRKALVITNSVKRESTVGEMVNLMSVDAQRFM
  DVSPFINLLWSAPLQVILAIYFLWQILGPSALAGVAVIVLLIPLNGAVSMKMKTYQVKQMKFKDSRIKLM
  SEILNGIKVLKLYAWEPSFLEQVKGIRQSELQLLRKGAYLQAISTFIWICTPFLVTLITLGVYVYVDESN
  VLDAEKAFVSLSLFNILKIPLNMLPQLISGLTQASVSLKRIQDFLNQNELDPQCVERKTISPGYAITIHN
  GTFTWAQDLPPTLHSLNIQIPKGALVAVVGPVGCGKSSLVSALLGEMEKLEGVVSVKGSVAYVPQQAWIQ
  NCTLQENVLFGQPMNPKRYQQALETCALLADLDVLPGGDQTEIGEKGINLSGGQRQRVSLARAVYSDANI
  FLLDDPLSAVDSHVAKHIFDQVIGPEGVLAGKTRVLVTHGISFLPQTDFIIVLAGGQVSEMGHYSALLQH
  DGSFANFLRNYAPDEDQEDHEAALQNANEEVLLLEDTLSTHTDLTDNEPAIYEVRKQFMREMSSLSSEGE
  VQNRTMPKKHTNSLEKEALVTKTKETGALIKEEIAETGNVKLSVYWDYAKSMGLCTTLSICLLYGGQSAA
  AIGANVWLSAWSNDAEEHGQQNKTSVRLGVYAALGILQGLLVMLSAFTMVVGAIQAARLLHEALLHNKIR
  SPQSFFDTTPSGRILNRFSKDIYVIDEVLAPTILMLLNSFFTSISTIMVIVASTPLFMVVVLPLAVLYGF
  VQRFYVATSRQLKRLESISRSPIFSHFSETVTGTSVIRAYGRIQDFKVLSDTKVDNNQKSSYPYIASNRW
  LGVHVEFVGNCVVLFAALFAVIGRNSLNPGLVGLSVSYALQVTMALNWMIRMISDLESNIIAVERVKEYS
  KTKTEAPWVVESNRAPEGWPTRGMVEFRNYSVRYRPGLELVLKNVTVHVQGGEKVGIVGRTGAGKSSMTL
  CLFRILEAAEGEIVIDGLNVAHIGLHDLRSQLTIIPQDPILFSGTLRMNLDPFGRYSEEDIWRALELSHL
  NTFVSSQPAGLDFQCAEGGDNLSVGQRQLVCLARALLRKSRVLVLDEATAAIDLETDDLIQGTIRTQFED
  CTVLTIAHRLNTIMDYNRVLVLDKGVVAEFDSPVNLIAAGGIFYGMAKDAGLA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029600
• ORF Size: 4566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029600.3, NP_083876.3
• RefSeq Size: 5002 bp
• RefSeq ORF: 4569 bp
• Locus ID: 76408
• UniProt ID: B2RX12
• Cytogenetics: 11 D
• Gene Summary: May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.[UniProtKB/Swiss-Prot Function]