Dzip1l (NM_028258) Mouse Tagged ORF Clone

CAT#: MG224313

Dzip1l (tGFP-tagged) - Mouse DAZ interacting protein 1-like (Dzip1l), (10ug)

Product Images

Circular map for MG224313

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dzip1l
• Synonyms: 2610524A10Rik; AI663976
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224313 representing NM_028258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTACCCAGCAGCCACTGCGGAAGGCCTTAGTGGCCCGCTGTCCGGGGCCTACACGCTTCCCGCCT
  TCAAGTTCCAGCCTCGCCGTGAAAGCATAGACTGGAGACGAATCAGTGCCGTGGACGTGGACCGTGTGGC
  CCGGGAGCTGGACGTGGCCACACTGCAGGAGAACATTGCTGGCGTCACCTTCTGTAACCTGGACGGGGAG
  GTGTGCAACCACTGTAGGCAGCCGGTGGACCCGGTGCTGCTCAAGGTACTGCGCCTGGCACAGCTCATCA
  TTGAGTACCTGCTGCATTGCCAGGACTGCCTGAGTGCCAGCGTGGCACAGCTGGAAGCACGGCTGCAGGC
  CAGCCTGGGCCAGCAGCAGCGTGGCCAGCAGGAGCTGGGTCGCCAGGCCGATGAGCTCAAGGGCGTTCGG
  GAGGAGAGCCGACGTAGGCGAAAGATGATCAGCACCCTGCAGCAGCTGCTTCTGCAGACGAGTGCCCATA
  GCTACCACACGTGCCACTTGTGTGACAAGACCTTCATGAATGCCACCTTTCTCCGGGGCCACATCCAACG
  CAGACATGCAGGCATGGCAGATGTCGGAAAGCAGAAGCAGGAACAGCCACTGGGGGAGGTGCTGGAGGAG
  CTTCGGGCCAAGCTCAAGTGGACCCAAGGGGAGCTGGAAGCCCAGAGGGAGGCTGAGCGGCAGCGGCAGG
  TCCAGGAATTAGAGATGGCTCGTCAGCGGGAAATGGAAGCTAAAAAAAAGTTCGACGAATGGAAAGAAAA
  AGAACGCAGCAAGCTTTATGGAGAGATAGACAAGCTGAAACAGCTGTTTTGGGATGAATTCAAAACTGTT
  GCCAACCAGAACTCCACGTTAGAAGAGAAACTGAAGGCACTGCAGTCCTACAGCATGACGGAGTCTCACC
  TTGGGTCTCTGCGGGATGAAGAGTCAGAGGAGAGACTCAAGCATGCCCAGGAGGTCCAGGCCCTTCAAGA
  AAAGATGGAAGTCCAGAAAACAGAGTGGAAGAGGAAAATGAAGGCCCTGCATGAAGAGCGGGCAGCTGAG
  AGGAGACAGCTGCAGGAGGAGAATGAGAGGCTCCACGTGTCCCTGTCTCAGGACCAGAAGAAAGCAGCTG
  CCCAGTCTCAGCGCCATATCAATGCCCTCCGTGCCCAGCTTCAAGAACAAGCCAGGCTTATCGAGTCCCA
  GGAGGAGACGATCCAGACTTTGTCCCTCAGGAAGGTGGAGGAAGTCCAAGAGATGCCAAAGGCTGTGGCC
  ACAGAGGAGGACTCTTCTGAAGAAGAGCTCGAGGCCTCCCTTGAGGAGCGGCAGGAGCAACGGAAGGTGT
  TAGCAGCTCTCAGGAAAAACCCCACTTGGCTGAAGCAGTTCCGGCCCATCCTGGAGGACACCCTGGAAGA
  GAAGCTGGAGGGCCTGGGGATCAAGAGGGACACAAAGGGAATTTCTGCTCAGACTGTCCGACGTCTGGAG
  CCCCTGCTGAGAACCCAAAGGGAGCAGATAGCCCGAAGCTTCCGAGAGTTTCCCAGTCTGAGGGAAAAGC
  TCAACAAGGAAGTGAGCAGCAGGGTGAAGCAAAGATGGGAGAGTACGACCCAGCCAGATGGCCAGCCTCC
  AGTCAAAAGCCAGCGGGTCACACTGGCCACAAGGGAGGTCCGGCCAAAGACCAGGACCTTGACTGTGGCC
  CTGCCATCCAAACCAGCAGAACCTTCAACGCCAACTCTTCAGGGCCACAGCAGCCATGGCCCCGGCCTCA
  CCCAGGTGTCCACCCCCATTCCACGCCCCAGAGTGCATGGACCCTCCAGTACCCCTGTTTCCCCTGGGTC
  CGGGCTGAGCAGCACCCCGCCTTTTAGTTCTGAAGAAGAGCCAGAGGGAGATGTCGTGCAACGTGTGTCC
  CTTCAGCCTCCTAAAGTTCTCCCTAGGTCAGCGGCCAAGCCTGAGGACAACTGGGGCTGGTCTGATTCCG
  AGACCTCAGAGGAGAGTGCCCAACCTCCTGGGAAGGGCTCAGGCGGGCTGGCTTCTTCAGGAACACTGGT
  GCAGTCAATTGTCAAAAACCTTGAGAAGCAGCTTGAAACGCCAGCCAAGAAGCCTTCCGGAGGGGTCAAT
  ATGTTCTTAAGGCCCAACGCTGCCCTCCAGAGGGCTTCCACACCAGCAAGGAAGTCTCAGCTTTCAGAAG
  ATGAGAGTGACGTGGAGATCTCGTCTTTGGAAGATCTCTCCCAAGACCTGGGCCAGAAAGGAAAGCCAAA
  GCCTTTGTCCCACTCAAAGCTCCCAGAAAAGTTTGATGTCAGCCCTTGGAGCTCGGGGTCACGACCCAGG
  ATTCCTGGCTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224313 representing NM_028258
  Red=Cloning site Green=Tags(s)
  
  MQYPAATAEGLSGPLSGAYTLPAFKFQPRRESIDWRRISAVDVDRVARELDVATLQENIAGVTFCNLDGE
  VCNHCRQPVDPVLLKVLRLAQLIIEYLLHCQDCLSASVAQLEARLQASLGQQQRGQQELGRQADELKGVR
  EESRRRRKMISTLQQLLLQTSAHSYHTCHLCDKTFMNATFLRGHIQRRHAGMADVGKQKQEQPLGEVLEE
  LRAKLKWTQGELEAQREAERQRQVQELEMARQREMEAKKKFDEWKEKERSKLYGEIDKLKQLFWDEFKTV
  ANQNSTLEEKLKALQSYSMTESHLGSLRDEESEERLKHAQEVQALQEKMEVQKTEWKRKMKALHEERAAE
  RRQLQEENERLHVSLSQDQKKAAAQSQRHINALRAQLQEQARLIESQEETIQTLSLRKVEEVQEMPKAVA
  TEEDSSEEELEASLEERQEQRKVLAALRKNPTWLKQFRPILEDTLEEKLEGLGIKRDTKGISAQTVRRLE
  PLLRTQREQIARSFREFPSLREKLNKEVSSRVKQRWESTTQPDGQPPVKSQRVTLATREVRPKTRTLTVA
  LPSKPAEPSTPTLQGHSSHGPGLTQVSTPIPRPRVHGPSSTPVSPGSGLSSTPPFSSEEEPEGDVVQRVS
  LQPPKVLPRSAAKPEDNWGWSDSETSEESAQPPGKGSGGLASSGTLVQSIVKNLEKQLETPAKKPSGGVN
  MFLRPNAALQRASTPARKSQLSEDESDVEISSLEDLSQDLGQKGKPKPLSHSKLPEKFDVSPWSSGSRPR
  IPGW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028258
• ORF Size: 2322 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028258.2
• RefSeq Size: 3928 bp
• RefSeq ORF: 2325 bp
• Locus ID: 72507
• UniProt ID: Q499E4
• Cytogenetics: 9 E3.3
• Gene Summary: Involved in primary cilium formation (PubMed:28530676). Probably acts as a transition zone protein required for localization of PKD1/PC1 and PKD2/PC2 to the ciliary membrane (By similarity).[UniProtKB/Swiss-Prot Function]