Bhlha15 (NM_010800) Mouse Tagged ORF Clone

CAT#: MG224224

Bhlha15 (tGFP-tagged) - Mouse basic helix-loop-helix family member a15 (Bhlha15), (10ug)

Product Images

Circular map for MG224224

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bhlha15
• Synonyms: 1810009C13Rik; Bhlhb8; MIST-1; Mist1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224224 representing NM_010800
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACCAAAAACCGGCCCCCTCGGCGCCGAACACCCATGCAGGACACAGAAGCCACCCCAGGAGAGC
  AGACACCTGACAGACCCCAGTCAGGCTCAGGGGGGTCAGAGCTGACAAAGGGTCTCCGGAGCAGGACAGC
  GCGTGCAAGCGGAGGTCGGGGAGAGGTCAGCCGCCGGCGACAGGGGTCTGGTGGCCGCAGGGAGAACAGT
  GTTCAGAGGCGGCTGGAGAGCAATGAGCGAGAGAGGCAGCGGATGCATAAACTCAACAATGCCTTCCAGG
  CACTGCGCGAGGTCATCCCGCACGTGCGGGCTGACAAGAAGCTCTCCAAGATCGAGACCCTCACGCTGGC
  CAAGAACTATATCAAGTCGCTGACCGCCACCATACTTACTATGTCCAGCAGCCGCCTCCCGGGGCTGGAG
  GCACCAGGTCCTGCGCCAGGCCCTAAATTATACCAGCACTACCATCACCAGCAGCAGCAACAGCAGCAGC
  AGCAGCAGGTAGCTGGGGCCATGCTTGGTGTCACTGAGGACCAGCCCCAAGGCCACCTGCAACGCTACTC
  TACACAGATCCACAGCTTCAGAGAGGGGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224224 representing NM_010800
  Red=Cloning site Green=Tags(s)
  
  MKTKNRPPRRRTPMQDTEATPGEQTPDRPQSGSGGSELTKGLRSRTARASGGRGEVSRRRQGSGGRRENS
  VQRRLESNERERQRMHKLNNAFQALREVIPHVRADKKLSKIETLTLAKNYIKSLTATILTMSSSRLPGLE
  APGPAPGPKLYQHYHHQQQQQQQQQQVAGAMLGVTEDQPQGHLQRYSTQIHSFREGS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010800
• ORF Size: 3491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010800.4, NP_034930.1
• RefSeq ORF: 594 bp
• Locus ID: 17341
• UniProt ID: Q9QYC3
• Cytogenetics: 5 G2
• Gene Summary: Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated (PubMed:17612490). Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.[UniProtKB/Swiss-Prot Function]