Acin1 (NM_023190) Mouse Tagged ORF Clone

CAT#: MG224187

Acin1 (tGFP-tagged) - Mouse apoptotic chromatin condensation inducer 1 (Acin1) transcript variant 2, (10ug)

Product Images

Circular map for MG224187

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acin1
• Synonyms: 2610036I19Rik; 2610510L13Rik; Acinus; acinusL; acinusS; Acn; C79325; mKIAA0670
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224187 representing NM_023190
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGACGGAAACGACCGAACAGCTCCGGAGAAACCCGGGGAATTCTGAGCGGTAATAGAGGGGTAG
  ACTATGGCAGTGGGCGGGGCCAATCCGGTCCGTTCGAAGGGCGTTGGCGGAAATTACCGAAGATGCCCGA
  AGCCGTCGGGACGGACCCGAGTACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTGGACGGGAAG
  CCTCTTCAGGCTCTGCGGGTGACCGACCTGAAGGCCGCACTGGAGCAGCGAGGCCTAGCCAAGAGCGGGC
  AGAAGAGTGCCCTGGTCAAGCGGCTCAAAGGGGCTCTAATGCTAGAAAATTTACAAAAACACTCAACACC
  CCATGCTGCATTCCAGCCAAATTCACAGATCGGGGAGGAAATGAGCCAGAACAGCTTCATAAAGCAGTAT
  CTGGAAAAGCAGCAGGAGCTACTTAGGCAGCGCCTGGAACGGGAAGCTCGGGAGGCTGCGGAACTTGAAG
  AAGCCTCAGCTGAGTCGGAGGACGAGATGACCCATCCTGAGGGAGTGGCTTCCCTGCTGCCTCCTGATTT
  TCAGAGCAGCCTGAACAGACCAGAGCTGGAGCTCAGCACACATTCACCCCGAAAGAGCTCCTCTTTTTCT
  GAAGAAAAAGGAGAATCTGATGATGAGAAGCCGAGGAAAGGGGAAAGACGATCATCCAGAGTTAGACAGG
  CAAAATCTAAACTCCCTGAGTACAGTCAGACTGCTGAGGAGGAAGAGGATCAAGAGACACCATCTAGAAA
  CCTGAGGGTCAGAGCAGATCGAAATTTAAAAATAGAGGAGGAGGAAGAAGAAGAGGAAGAAGAAGAAGAT
  GACGATGATGAAGAGGAAGAGGAGGTAGATGAGGCACAGAAATCTAGAGAGGCTGAGGCACCAACCCTGA
  AGCAGTTTGAGGATGAAGAAGGGGAAGAGAGGACCAGAGCAAAACCAGAAAAGGTGGTGGATGAGAAACC
  CCTAAACATCAGATCCCAGGAAAAGGGTGAGTTAGAGAAAGGAGGGCGAGTTACTAGATCCCAAGAAGAA
  GCTAGAAGAAGTCATCTGGCCAGACAGCAACAAGAGAAAGAGACACAAATTGTTTCTCTCCCTCAAGAAG
  AAAATGAGGTAAAGTCTTCACAAAGTTTGGAGGAAAAATCCCAGTCTCCCTCCCCACCTCCGCTTCCTGA
  AGACCTAGAGAAGGCCCCTGTTGTGCTGCAGCCAGAACAGATAGTCAGTGAAGAGGAAACACCCCCACCT
  TTACTTACCAAGGAAGCGTCATCTCCACCAACTCATATACAACTCCAGGAAGAGATGGAGCCAGTGGAAG
  GCCCAGCACCCCCTGTCCTCATTCAGTTGTCTCCTCCTAACACAGATGCTGGAGCCAGGGAGCCACTAGC
  GTCTCCTCACCCTGCGCAGCTGCTAAGAAGCCTGTCTCCTTTGTCAGGTACTACAGACACCAAAGCAGAG
  TCTCCAGCAGGGAGAGTGTCAGATGAGAGTGTCCTGCCTCTGGCTCAGAAAAGCTCACTGCCTGAATGCT
  CCACCCAGAAGGGTGTTGAAAGTGAGCGTGAAAAGTCTGCTCCCCTCCCTCTAACAGTGGAGGAGTTCGC
  ACCTGCCAAAGGCATCACTGAGGAACCTATGAAGAAACAGTCTTTGGAACAGAAGGAGGGCAGAAGAGCC
  TCCCATGCTCTCTTCCCAGAGCACAGTGGGAAGCAGTCAGCCGACTCATCCTCTAGCAGGTCCTCCTCCC
  CTTCATCCTCCAGCTCTCCATCCAGATCTCCATCTCCTGACAGTGTGGCTTCCCGCCCACAGTCGTCTCC
  AGGATCTAAGCAGAGAGATGGAGCCCAAGCACGAGTTCATGCCAACCCTCACGAGAGACCCAAGATGGGC
  TCCAGGTCAACTTCAGAGTCCAGGTCACGTTCCCGATCTCGTTCCCGTTCCGCGTCAAGCAGCAGCAGAA
  AATCTCTGAGCCCTGGAGTCTCCAGAGATAGCAACACCAGCTACACTGAAACCAAAGATCCCTCTTGTGG
  TCAGGAGGCTGCAGCTCCATCTGGGCCACAGCTGCAGGTCCTTGAACCAAAGGAGAAGGCTCCCACGTTC
  TCAGCATCTGTCCGGGGGAGGCATCTGAGCCATCCTGAGCCAGAGCAACAGCACGTGATCCAGAGGTTGC
  AGCCGGAGCAGGGGAGCCCAAAGAAGTGTGAAGCTGAAGAGGCAGAGCCACCAGCTGCCACACAGCCTCA
  AACCTCAGAGACTCAGATCTCTCATCTGCTAGAATCAGAAAGGACTCATCACACTGTTGAAGAGAAGGAG
  GAAGTGACCATGGACACCAGTGAAAACAGACCTGAAAATGAGGTGCCTGAGCCTCCTCTGCCTGTTGCAG
  ACCAAGTCAGCAATGATGAGCGCCCAGAGGGTGGTGCTGAAGAAGAGGAAAAGAAAGAGAGTTCGATGCC
  CAAGTCATTCAAGAGGAAAATCTCCGTTGTCTCAGCTACCAAGGGGGTGCAAGCTGGAAACAGTGACACA
  GAGGGGGGCCAGCCTGGCCGAAAACGCCGTTGGGGAGCCAGCACTGCCGCGACACAGAAGAAACCGTCCA
  TCAGTATCACCACTGAGTCACTCAAGAGCCTCATCCCCGACATCAAACCCCTGGCGGGGCAGGAGGCTGT
  TGTGGATCTTCATGCCGATGACTCCCGAATCTCTGAGGATGAGACAGAGCGTAATGGCGACGATGGGACC
  CATGACAAGGGACTGAAGATATGCCGGACAGTCACTCAGGTAGTACCCGCAGAGGGCCAGGAGAATGGGC
  AGAGGGAAGAGGAAGAAGAGAAAGAGCCTGAAGCCGAGCTGCCGGCGCCACCCCAGGTGTCAGTGGAGGT
  TGCCTTGCCCCCACCTGTGGAGCACGAAGTAAAGAAAGTAACATTAGGAGATACCTTAACCCGGAGGTCC
  ATCAGCCAACAGAAGTCTGGAGTTTCCATTACAATTGATGACCCAGTCCGGACCGCCCAGGTGCCCTCCC
  CACCCAGGGGCAAGATCAGTAACATTGTCCACATCTCCAACCTGGTTCGTCCCTTCACTTTAGGCCAGCT
  GAAGGAATTATTGGGGCGTACAGGAACTTTGGTGGAAGAGGCCTTCTGGATAGACAAGATCAAATCTCAT
  TGCTTTGTGACGTACTCTACAGTAGAGGAAGCCGTTGCCACCCGCACAGCTCTGCACGGGGTCAAGTGGC
  CCCAGTCCAACCCCAAATTCCTTTGTGCTGACTATGCTGAGCAAGATGAGCTGGACTATCACCGGGGACT
  CTTGGTAGATCGGCCATCTGAAACTAAGGCAGAGGAACAGGGAGCACCAAGGCCCCTGCATCCCCCACCC
  CCACCCCCAGTCCAGCCACCGCCCCACCCCCGGGCTGAGCAGCGGGAGCAGGAAAGGGCTGTTCGAGAGC
  AATGGGCAGAACGGGAACGGGAAATGGAGCGCCGGGAGAGGACTCGGTCTGAGAGAGAATGGGATCGGGA
  CAAAGTTCGAGAGGGACCCCGCTCCCGATCACGGTCCCGTGACCGCCGCCGGAAAGAGCGAGCAAAATCT
  AAAGAAAAGAAGAGTGAAAAGAAAGAAAAAGCCCAGGAGGAGCCACCTGCCAAGCTGCTGGATGACCTCT
  TCCGTAAGACTAAGGCAGCTCCCTGCATCTATTGGCTCCCTCTGACTGAGAGCCAAATTGTTCAGAAGGA
  GGCAGAGCAAGCTGAACGGGCCAAGGAGCGGGAGAAGCGGCGAAAAGAACGAGAAGAAGAAGAACAAAAG
  GAACGGGAGAAGGAAGCTGAGCGGGAACGGAACCGGCAGCTAGAACGGGAGAAGAGGAGGGAGCACAGCA
  GGGAGAGAGAGAGGGACAGGGAGAGAGAGCGGGACAGGGGTGACCGAGAGCGGGAGAGGGAGCGAGACCG
  AGACCGAGGCAGGGAGAGGGATCGCAGAGACACCAAGCGCCACAGCAGAAGCCGGAGTCGAAGCACACCT
  GTACGGGACCGGGGTGGGCGCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224187 representing NM_023190
  Red=Cloning site Green=Tags(s)
  
  MWGRKRPNSSGETRGILSGNRGVDYGSGRGQSGPFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGK
  PLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQY
  LEKQQELLRQRLEREAREAAELEEASAESEDEMTHPEGVASLLPPDFQSSLNRPELELSTHSPRKSSSFS
  EEKGESDDEKPRKGERRSSRVRQAKSKLPEYSQTAEEEEDQETPSRNLRVRADRNLKIEEEEEEEEEEED
  DDDEEEEEVDEAQKSREAEAPTLKQFEDEEGEERTRAKPEKVVDEKPLNIRSQEKGELEKGGRVTRSQEE
  ARRSHLARQQQEKETQIVSLPQEENEVKSSQSLEEKSQSPSPPPLPEDLEKAPVVLQPEQIVSEEETPPP
  LLTKEASSPPTHIQLQEEMEPVEGPAPPVLIQLSPPNTDAGAREPLASPHPAQLLRSLSPLSGTTDTKAE
  SPAGRVSDESVLPLAQKSSLPECSTQKGVESEREKSAPLPLTVEEFAPAKGITEEPMKKQSLEQKEGRRA
  SHALFPEHSGKQSADSSSSRSSSPSSSSSPSRSPSPDSVASRPQSSPGSKQRDGAQARVHANPHERPKMG
  SRSTSESRSRSRSRSRSASSSSRKSLSPGVSRDSNTSYTETKDPSCGQEAAAPSGPQLQVLEPKEKAPTF
  SASVRGRHLSHPEPEQQHVIQRLQPEQGSPKKCEAEEAEPPAATQPQTSETQISHLLESERTHHTVEEKE
  EVTMDTSENRPENEVPEPPLPVADQVSNDERPEGGAEEEEKKESSMPKSFKRKISVVSATKGVQAGNSDT
  EGGQPGRKRRWGASTAATQKKPSISITTESLKSLIPDIKPLAGQEAVVDLHADDSRISEDETERNGDDGT
  HDKGLKICRTVTQVVPAEGQENGQREEEEEKEPEAELPAPPQVSVEVALPPPVEHEVKKVTLGDTLTRRS
  ISQQKSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSH
  CFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKAEEQGAPRPLHPPP
  PPPVQPPPHPRAEQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKS
  KEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTESQIVQKEAEQAERAKEREKRRKEREEEEQK
  EREKEAERERNRQLEREKRREHSRERERDRERERDRGDRERERERDRDRGRERDRRDTKRHSRSRSRSTP
  VRDRGGRR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023190
• ORF Size: 4014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023190.3, NP_075679.2
• RefSeq Size: 4751 bp
• RefSeq ORF: 4017 bp
• Locus ID: 56215
• UniProt ID: Q9JIX8
• Cytogenetics: 14 C2
• Gene Summary: Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells (By similarity).[UniProtKB/Swiss-Prot Function]