Senp3 (NM_030702) Mouse Tagged ORF Clone

CAT#: MG224185

Senp3 (tGFP-tagged) - Mouse SUMO/sentrin specific peptidase 3 (Senp3) transcript variant 1, (10ug)

Product Images

Circular map for MG224185

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Senp3
• Synonyms: AA408656; Smt3ip; Smt3ip1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224185 representing NM_030702
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGAGACTATACAGGGGACCGGGTCTTGGGGGCCTGAGCCTCCGGGACCCGGCACCACTTACTCAA
  ATCCCAGGCGAGAGCGTCTTCGTTGGCCCCTACCCCCTAAGCCCCGGCTCAAGTCCGGTGGTGGTTTTGG
  GCCAGATCCTGGGTCTGGGACCACAGTGCCAACTAGACGCCTCCCTGCCCCCCGGCCATCTTTTGATGCC
  TCAGCTAGTGAAGAAGAGGAAGAGGAAGAGGAGGAAGATGAGGAGGAAGTAGCAGCTTGGAGGCTACCCC
  CTAGGTGGGGCCAACTGGGGGCCTCCCAGCGCTCTCGAGCTCTCCGACCCTCTCATAGAAAAACCTGCTC
  ACAGCGCCGGCGCCGAGCCATGAGAGCCTTCCAGATGCTGCTCTACTCAAAAAGCACCTCGCTGACATTC
  CACTGGAAGCTTTGGGGGCGCCACCGAGGCCGGCGGCGGAACCTCGCGCACCCCAAGAACCATCTCTCAC
  CCCAGGAAGGGGGTGCAACGCCACAGGTGCCATCACCCTGCTGTCGTTTTGACTCCCCCCGGGGGCTACC
  CCCACCCCGGCTGGGTCTGCTAGGTGCTCTCATGGCAGAGGATGGGATGAGAGGGTCTCCACCAGTGCCC
  TCTGGGCCCCCCATGGAGGAAGATGGACTAAGGTGGACCCCAAAGTCTCCTCTGGACCCTGACTCGGGCC
  TCCTCTCATGTACTCTACCCAATGGTTTTGGGGGACTGTCTGGTCCTGAAGGGGAGCGCAGCCTGGCACC
  CCCTGATGCCAGCATACTCATCAGTAATGTGTGCAGCATTGGAGACCACGTGGCTCAGGAACTTTTTCAG
  AGCTCTGACTTGGGCATTGCAGAAGAGGCAGATCGGACTGGGGAGAAAGCTGGCCAGCATAGCCCCCTTC
  GGGAGGAACATGTGACCTGTGTGCAGAGTATCTTAGATGAATTCCTTCAAACTTATGGCAGCCTCATCCC
  TCTCAGCACTGATGAAGTTGTAGAGAAGTTGGAGGACATTTTCCAGCAGGAGTTCTCTACACCCTCAAGG
  AAGAGTCTGGTACTACAGCTGATCCAGTCTTATCAGCGGATGCCAGGCAACGCTATGGTAAGGGGCTTCC
  GGGTATCCTATAAGCGACACGTGCTCACCATGGATGACTTGGGTACCTTATATGGACAGAACTGGCTCAA
  TGACCAGGTGATGAACATGTATGGAGATCTGGTCATGGACACAGTCCCTGAAAAGGTGCATTTCTTCAAC
  AGCTTCTTCTATGATAAACTCCGTACCAAGGGTTATGATGGGGTAAAGAGGTGGACCAAAAATGTGGACA
  TCTTCAATAAGGAATTACTGCTAATCCCCATCCATCTGGAGGTGCACTGGTCCCTTATCTCAGTTGATGT
  AAGGCGACGTACCATCACCTATTTTGACTCCCAGCGAACTCTAAATCGCCGCTGCCCTAAGCATATTGCC
  AAGTATCTACAGGCAGAGGCAGTCAAAAAAGACCGACTGGACTTCCATCAGGGCTGGAAAGGTTACTTCA
  AAATGAATGTGGCCAGGCAGAATAATGACAGTGACTGTGGTGCCTTTGTATTACAGTACTGCAAGCACCT
  GGCCCTGTCTCAGCCATTCAGCTTCACCCAGCAGGACATGCCCAAACTCCGTCGTCAGATCTACAAGGAA
  CTGTGTCACTGCAAACTCACTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224185 representing NM_030702
  Red=Cloning site Green=Tags(s)
  
  MKETIQGTGSWGPEPPGPGTTYSNPRRERLRWPLPPKPRLKSGGGFGPDPGSGTTVPTRRLPAPRPSFDA
  SASEEEEEEEEEDEEEVAAWRLPPRWGQLGASQRSRALRPSHRKTCSQRRRRAMRAFQMLLYSKSTSLTF
  HWKLWGRHRGRRRNLAHPKNHLSPQEGGATPQVPSPCCRFDSPRGLPPPRLGLLGALMAEDGMRGSPPVP
  SGPPMEEDGLRWTPKSPLDPDSGLLSCTLPNGFGGLSGPEGERSLAPPDASILISNVCSIGDHVAQELFQ
  SSDLGIAEEADRTGEKAGQHSPLREEHVTCVQSILDEFLQTYGSLIPLSTDEVVEKLEDIFQQEFSTPSR
  KSLVLQLIQSYQRMPGNAMVRGFRVSYKRHVLTMDDLGTLYGQNWLNDQVMNMYGDLVMDTVPEKVHFFN
  SFFYDKLRTKGYDGVKRWTKNVDIFNKELLLIPIHLEVHWSLISVDVRRRTITYFDSQRTLNRRCPKHIA
  KYLQAEAVKKDRLDFHQGWKGYFKMNVARQNNDSDCGAFVLQYCKHLALSQPFSFTQQDMPKLRRQIYKE
  LCHCKLTV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030702
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030702.4, NP_109627.3
• RefSeq Size: 2442 bp
• RefSeq ORF: 1707 bp
• Locus ID: 80886
• UniProt ID: Q9EP97
• Cytogenetics: 11 B3
• Gene Summary: Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.[UniProtKB/Swiss-Prot Function]