Hmbox1 (NM_177338) Mouse Tagged ORF Clone

CAT#: MG224083

Hmbox1 (tGFP-tagged) - Mouse homeobox containing 1 (Hmbox1), (10ug)

Product Images

Circular map for MG224083

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hmbox1
• Synonyms: AI451877; AI604847; F830020C16Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224083 representing NM_177338
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAGCTCCTTTCCAGTGGTTTTGCTGGAAACCATGTCTCACTACACAGATGAACCCAGATTTACCA
  TAGAACAGATAGACCTGCTCCAGCGTCTTCGGCGTACCGGGATGACCAAACATGAAATCCTTCATGCATT
  AGAAACTTTGGACCGTCTTGATCAAGAGCATAGTGATAAATTTGGAAGGAGGTCCAGCTACGGGGGAAGC
  TCATATGGGAACAGTACCAACAACGTTCCAGCATCTTCCTCTACAGCCACAGCTTCCACGCAGACCCAGC
  ACTCGGGAATGTCCCCATCACCCAGCAACAGTTACGATACCTCCCCACTGCCTTGCACTACCAATCAAAA
  TGGGAGGGAGAACAATGATCGATTGTCCACATCCAATGGGAAGATGTCACCATCTCGCTACCATGCAAAC
  AGCATGGGTCAGAGGTCATATAGCTTTGAGGCCTCAGAAGAGGACCTAGATGTAGATGATAAAGTGGAGG
  AATTAATGAGGAGGGACAGCAGTGTGATAAAAGAGGAAATCAAAGCCTTTCTTGCCAATCGGAGGATTTC
  CCAAGCAGTTGTTGCACAGGTAACAGGAATCAGTCAGAGTCGAATCTCTCACTGGCTGCTGCAGCAGGGA
  TCAGATCTGAGTGAGCAGAAGAAAAGGGCGTTCTACCGATGGTATCAACTTGAGAAGACAAACCCTGGGG
  CTACGCTAAGTATGAGACCTGCCCCCATTCCAATAGAGGACCCTGAATGGAGACAAACACCTCCCCCAGT
  CTCCGCCACACCTGGAACCTTCCGGCTTCGACGAGGGAGTAGATTTACCTGGAGAAAGGAGTGCCTAGCT
  GTCATGGAAAGTTACTTCAATGAGAACCAGTACCCAGATGAAGCAAAGAGAGAAGAAATTGCCAATGCTT
  GCAATGCAGTCATACAGAAGCCAGGCAAAAAGCTGTCTGACCTGGAACGAGTTACCTCTCTGAAAGTATA
  TAATTGGTTTGCTAATCGACGGAAGGAGATCAAGAGAAGAGCCAATATCGCAGCAATCCTGGAGAGTCAT
  GGGATAGATGTACAGAGTCCAGGAGGCCATTCCAACAGTGACGATGTGGACGGGAACGACTACTCCGAGC
  AGGATGACAGCACCAGCCATAGTGACCACCAAGATCCCATCTCGCTAGCTGTGGAGATGGCGGCCGTCAA
  CCACACTATCTTGGCATTGGCCCGGCAGGGAGCCAATGAAATCAAGACAGAGGCCCTGGATGATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224083 representing NM_177338
  Red=Cloning site Green=Tags(s)
  
  MLSSFPVVLLETMSHYTDEPRFTIEQIDLLQRLRRTGMTKHEILHALETLDRLDQEHSDKFGRRSSYGGS
  SYGNSTNNVPASSSTATASTQTQHSGMSPSPSNSYDTSPLPCTTNQNGRENNDRLSTSNGKMSPSRYHAN
  SMGQRSYSFEASEEDLDVDDKVEELMRRDSSVIKEEIKAFLANRRISQAVVAQVTGISQSRISHWLLQQG
  SDLSEQKKRAFYRWYQLEKTNPGATLSMRPAPIPIEDPEWRQTPPPVSATPGTFRLRRGSRFTWRKECLA
  VMESYFNENQYPDEAKREEIANACNAVIQKPGKKLSDLERVTSLKVYNWFANRRKEIKRRANIAAILESH
  GIDVQSPGGHSNSDDVDGNDYSEQDDSTSHSDHQDPISLAVEMAAVNHTILALARQGANEIKTEALDDD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177338
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177338.6
• RefSeq Size: 2931 bp
• RefSeq ORF: 1260 bp
• Locus ID: 219150
• UniProt ID: Q8BJA3
• Cytogenetics: 14 D1
• Gene Summary: Binds directly to 5'-TTAGGG-3' repeats in telomeric DNA (By similarity). Associates with the telomerase complex at sites of active telomere processing and positively regulates telomere elongation (By similarity). Important for TERT binding to chromatin, indicating a role in recruitment of the telomerase complex to telomeres (PubMed:23685356). Also plays a role in the alternative lengthening of telomeres (ALT) pathway in telomerase-negative cells where it promotes formation and/or maintenance of ALT-associated promyelocytic leukemia bodies (APBs) (By similarity). Enhances formation of telomere C-circles in ALT cells, suggesting a possible role in telomere recombination (By similarity). Might also be involved in the DNA damage response at telomeres (By similarity).[UniProtKB/Swiss-Prot Function]