Tbc1d9 (NM_027758) Mouse Tagged ORF Clone

CAT#: MG224010

Tbc1d9 (tGFP-tagged) - Mouse TBC1 domain family member 9 (Tbc1d9) transcript variant 2, (10ug)

Product Images

Circular map for MG224010

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tbc1d9
• Synonyms: 4933431N12Rik; AI847101; AW490653; C76116
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224010 representing NM_027758
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGTGAATCCGGAGGAGGTACTGCTGGCCAACGCGCTGTGGATCACCGAGAGAGCCAACCCCTACT
  TCATTCTGCAACGAAGGAAGGGCCACGGGGGCGACGGGGGTGGCGGCGGCGGATTGGCGGGCCTCCTTGT
  GGGTACCCTCGATGTGGTATTGGACTCCAGTGCCCGAGTCGCACCGTACCGAATCCTTTATCAAACTCCA
  GACTCTCTAGTCTATTGGACCATTGCCTGTGGTGGCTCCAGGAAGGAGGTTACTGAGCACTGGGAATGGC
  TTGAACAGAACCTACTGCAGACACTCTCCATCTTTGAGAATGAGAATGACGTCACCACATTTGTAAGAGG
  GAAAATACAGGTAACCATCGTGGAGAAGGCCGACAGCTGCAGTGTGCTCCCCAGCCCTCTGTCCATCAGC
  ACCAGGAACAGGATGACCTTCCTGTTCGCGAACTTGAAAGACAGAGACTTTCTGGTGCAGAGGATCTCAG
  ATTTCTTGCAGCAAACGACTTCTAGGATCTACTCAGACAAAGAGTTTTCAGGAAGCTGCAACAGCTCAGA
  TGATGAGGTGTATTCTCGGCCCAGCAGCCTCGTTTCCTCCAGTCCCCAGAGAAGCACAAGCTCGGATGCT
  GATGGAGAACGCCCCTTTAACCTCAATGGCAACAGTGTCCCTACAGCCACCCAGACCCTCATGACCATGT
  ACCGGCGGCGATCTCCTGAAGAATTCAACCCAAAATTGGCCAAAGAGTTTTTGAAAGAACAAGCCTGGAA
  GATTCACTTTGCTGAGTATGGGCAGGGAATCTGCATGTACCGCACAGAGAAGACCCGGGAGCTGGTGCTG
  AAGGGCATCCCAGAGAGCATGCGCGGAGAGCTCTGGCTGCTGCTTTCAGGGGCCATCAATGAAAAGGCCA
  CACACCCCGGGTACTACGAGGGCCTCGTGGAGAAGTCGATGGGAAAGTACAATCTGGCCACCGAGGAGAT
  TGAGAGAGACTTACACCGCTCCCTTCCAGAACACCCAGCTTTCCAGAACGAAATGGGCATCGCGGCTCTG
  AGGAGAGTCTTAACGGCTTATGCTTTTCGCAATCCCAACATAGGATACTGCCAGGCCATGAACATTGTCA
  CTTCAGTGCTACTGCTGTATGCCAAAGAGGAGGAGGCCTTCTGGCTGCTTGTGGCCTTGTGTGAGCGCAT
  GCTTCCCGACTACTACAACACCAGAGTTGTAGGTGCCCTGGTGGACCAGGGAGTCTTCGAGGAGCTGGCT
  CGAGACTATGTCCCGCAGCTGTACGACTGCATGCAGGACCTGGGGGTGATCTCTACCATCTCCCTGTCGT
  GGTTCCTCACACTCTTCCTCAGTGTGATGCCATTTGAGAGTGCAGTGGTGGTTGTGGACTGTTTCTTCTA
  TGAAGGAATCAAAGTGATATTTCAGCTGGCTCTCGCTGTACTGGATGCCAACGTGGATAAACTCTTGAAC
  TGCAAGGACGATGGAGAGGCCATGACTGTTTTGGGAAGGTACCTAGACAGTGTGACCAACAAAGACAGCA
  CCCTGCCTCCCATCCCCCATCTCCACTCCCTGCTCAGTGACGACGTGGGGCCCTACCCTGCCGTGGACAT
  CTTCAGACTCATAGGGACGTCTTACGAGAAATTTGGAACTATCCGGGCCGATTTGATTGAGCAGATGCGA
  TTCAAGCAGAGGCTGAAAGTGATCCAGACCCTGGAGGACACCACCAAACGCAATGTGGTACGGACCATTG
  TGACGGAGACGTCCTTCACCATCGATGAGCTGGAAGAACTGTACGCTCTTTTCAAGGCTGAGCATCTGAC
  CAGCTGCTACTGGGGTGGGAGCAGCAATGCCCTGGACCGACACGACCCCAGCCTGCCTTACCTGGAGCAG
  TACCGCATCGACTTTGAGCAGTTCAAGGGCATGTTCGTGCTGCTCTTTCCCTGGGCGTGTGGCACACACT
  CCGATGTGCTGGCCTCCCGCCTGTTCCAGCTGTTAGATGAGAATGGAGACTCCTTGATTAACTTCCGAGA
  GTTCGTCTCTGGGCTGAGTGCTGCATGCCATGGGGACCTCACAGAGAAACTCAAACTCCTGTACAAGATG
  CACGTCTTGCCTGAGCCATCCTGTGATCAAGATGAGCCTGACTCCGCCTTTGAAGCCACTCAGTACTTTT
  TTGAAGACATCACCCCAGAATGCACACATGTTGTTGGCTTGGACAGTAGAGGCAAGCAGAGTGCGGACGA
  CGGCTTCGTCACAGTGAGCCTGAAGCAGGACAGAGGGAAAAGGGCAAATTCTCAAGAAAACCGTAATTAC
  CTAAAACTCTGGACTGCAGAAAATAAGTCTAAGTCAAAAACTGCCAAGGATTTGCCCAAGCTGAACCAGG
  GGCAGTTTATTGAGCTCTGTAAGACCATGTACAACATGTTCAGTGAAGACCCCAATGAGCAGGAGCTGTA
  CCATGCCACAGCTGCGGTGACCAGCCTCCTGCTGGAGATCGGAGAGGTTGGCAAGTTCTTCATTACTCAG
  CCGGCAAAGGAAGATGCAGTTCCTGGACCACCCTGCGGCCAGGCCATCCCAGGCATGCTCTTCCCTAAGA
  AGGGGTCCAGCCAGTCCTATGTGGTGGAGTCCACAGAGCCACTCACAGCTAGCCTGGCAGTGGACAGTGA
  GGAGCACTCCCTGGGAGGGCAGATGGAGGACATTAAACTGGAGGACTCCTCACCCAGGGACAACGGGGCC
  TGCTCCTCCATGTTGATCTCCGATGATGACACCAAGGATGACAGCTCCATGTCATCCTACTCGGTGCTGA
  GCGCCGGCTCCCACGAGGAGGACAAACTGCACTGTGAGGACATTGGAGAGGACACAGTCCTGGTGCGTAG
  CAGTCAGGGTAGAGCCACCCTGCCCAGGAGCAGCAGCCTGGACCGGGACTGGGCCATCACCTTTGAGCAG
  TTCCTGGCCTCGCTCTTGACGGAGCCCGCTCTCGTGAGGTACTTTGACAAGCCAGTGTGCATGATGGCCA
  GGGTCACCAGTGCGAAGAACATCCGTATGATGGGCAAGCCCCTCACCTCAGCCAGTGACTATGAAATCTC
  TGCCCTGTCGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224010 representing NM_027758
  Red=Cloning site Green=Tags(s)
  
  MWVNPEEVLLANALWITERANPYFILQRRKGHGGDGGGGGGLAGLLVGTLDVVLDSSARVAPYRILYQTP
  DSLVYWTIACGGSRKEVTEHWEWLEQNLLQTLSIFENENDVTTFVRGKIQVTIVEKADSCSVLPSPLSIS
  TRNRMTFLFANLKDRDFLVQRISDFLQQTTSRIYSDKEFSGSCNSSDDEVYSRPSSLVSSSPQRSTSSDA
  DGERPFNLNGNSVPTATQTLMTMYRRRSPEEFNPKLAKEFLKEQAWKIHFAEYGQGICMYRTEKTRELVL
  KGIPESMRGELWLLLSGAINEKATHPGYYEGLVEKSMGKYNLATEEIERDLHRSLPEHPAFQNEMGIAAL
  RRVLTAYAFRNPNIGYCQAMNIVTSVLLLYAKEEEAFWLLVALCERMLPDYYNTRVVGALVDQGVFEELA
  RDYVPQLYDCMQDLGVISTISLSWFLTLFLSVMPFESAVVVVDCFFYEGIKVIFQLALAVLDANVDKLLN
  CKDDGEAMTVLGRYLDSVTNKDSTLPPIPHLHSLLSDDVGPYPAVDIFRLIGTSYEKFGTIRADLIEQMR
  FKQRLKVIQTLEDTTKRNVVRTIVTETSFTIDELEELYALFKAEHLTSCYWGGSSNALDRHDPSLPYLEQ
  YRIDFEQFKGMFVLLFPWACGTHSDVLASRLFQLLDENGDSLINFREFVSGLSAACHGDLTEKLKLLYKM
  HVLPEPSCDQDEPDSAFEATQYFFEDITPECTHVVGLDSRGKQSADDGFVTVSLKQDRGKRANSQENRNY
  LKLWTAENKSKSKTAKDLPKLNQGQFIELCKTMYNMFSEDPNEQELYHATAAVTSLLLEIGEVGKFFITQ
  PAKEDAVPGPPCGQAIPGMLFPKKGSSQSYVVESTEPLTASLAVDSEEHSLGGQMEDIKLEDSSPRDNGA
  CSSMLISDDDTKDDSSMSSYSVLSAGSHEEDKLHCEDIGEDTVLVRSSQGRATLPRSSSLDRDWAITFEQ
  FLASLLTEPALVRYFDKPVCMMARVTSAKNIRMMGKPLTSASDYEISALSG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027758
• ORF Size: 3093 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027758.4, NP_082034.1
• RefSeq Size: 5149 bp
• RefSeq ORF: 3096 bp
• Locus ID: 71310
• UniProt ID: Q3UYK3
• Cytogenetics: 8 C2
• Gene Summary: May act as a GTPase-activating protein for Rab family protein(s).[UniProtKB/Swiss-Prot Function]