Agpat1 (NM_001163379) Mouse Tagged ORF Clone

CAT#: MG223779

Agpat1 (tGFP-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase alpha) (Agpat1) transcript variant 1, (10ug)

Product Images

Circular map for MG223779

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Agpat1
• Synonyms: 1-AGP; 1-AGPAT; AW047140
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223779 representing NM_001163379
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTGGCCCGGGGCCTGGACGGCGCTGCTGCAGCTGCTGCTCCTGCTGCTGTCCACCCTGTGGT
  TCTGCAGCTCCAGTGCCAAGTATTTCTTTAAGATGGCCTTCTACAACGGCTGGATCCTCTTCCTGGCGAT
  CCTCGCAATCCCCGTGTGTGCTGTGCGAGGGCGCAATGTCGAGAACATGAAGATCTTGCGTCTGCTGCTG
  CTCCACGTCAAATACCTGTATGGGATCCGAGTGGAGGTGCGCGGGGCTCACCACTTCCCTCCCACGCAGC
  CCTACGTGGTTGTGTCCAACCACCAGAGTTCCCTCGACCTGCTTGGAATGATGGAGGTCCTGCCAGATCG
  TTGTGTGCCCATTGCCAAGCGTGAGCTACTATGGGCTGGCTCTGCTGGGCTGGCCTGCTGGCTGGCAGGA
  ATCATCTTCATTGACCGGAAACGCACAGGGGACGCCATCAGTGTCATGTCCGAGGTGGCTCAGACCTTGC
  TCACCCAGGATGTGAGAGTCTGGGTTTTTCCTGAAGGAACAAGAAACCACAATGGCTCCATGCTGCCCTT
  CAAACGAGGCGCCTTCCACCTTGCAGTACAGGCCCAGGTCCCCATCATCCCCATCGTCATGTCCTCCTAT
  CAAGACTTCTACTCCAAAAAGGAAAGACGCTTCACCTCGCCTGGACGTTGTCAGGTGCGGGTGCTGCCTC
  CAGTGTCCACAGAAGGGCTGACACCAGATGACGTCCCAGCTCTGGCAGACAGCGTCCGGCACTCCATGCT
  CACCATCTTCCGGGAGATCTCCACTGACGGCCTGGGTGGTGGCGACTGTCTGAAGAAGCCTGGGGGAGCG
  GGCGAAGCCCGGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223779 representing NM_001163379
  Red=Cloning site Green=Tags(s)
  
  MELWPGAWTALLQLLLLLLSTLWFCSSSAKYFFKMAFYNGWILFLAILAIPVCAVRGRNVENMKILRLLL
  LHVKYLYGIRVEVRGAHHFPPTQPYVVVSNHQSSLDLLGMMEVLPDRCVPIAKRELLWAGSAGLACWLAG
  IIFIDRKRTGDAISVMSEVAQTLLTQDVRVWVFPEGTRNHNGSMLPFKRGAFHLAVQAQVPIIPIVMSSY
  QDFYSKKERRFTSPGRCQVRVLPPVSTEGLTPDDVPALADSVRHSMLTIFREISTDGLGGGDCLKKPGGA
  GEARL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163379
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163379.1, NP_001156851.1
• RefSeq Size: 2190 bp
• RefSeq ORF: 858 bp
• Locus ID: 55979
• UniProt ID: O35083
• Cytogenetics: 17 18.18 cM
• Gene Summary: Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.[UniProtKB/Swiss-Prot Function]