Ube3a (NM_173010) Mouse Tagged ORF Clone

CAT#: MG223606

Ube3a (tGFP-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a) transcript variant 1, (10ug)

Product Images

Circular map for MG223606

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ube3a
• Synonyms: 4732496B02; 5830462N02Rik; A130086L21Rik; Hpve6a; mKIAA4216
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223606 representing NM_173010
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCATCAGTTAACTGAGGGCTGTGGAAATG
  AGGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAACAATGCAGCAGCTAT
  TAAAGCCCTTGAGCTTTATAAAATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGC
  TCAGCTTACCTTGAGAACTCAAAAGGTGCATCTAACAACTCAGAGATAAAAATGAACAAGAAGGAAGGAA
  AAGATTTTAAAGATGTGATTTACCTAACTGAAGAGAAAGTATATGAAATTTATGAATTTTGTAGAGAGAG
  TGAGGATTATTCCCCTTTAATTCGTGTAATTGGAAGAATATTTTCTAGTGCTGAGGCACTGGTTCTGAGC
  TTTCGGAAAGTCAAACAGCACACAAAGGAGGAATTGAAATCTCTTCAAGAAAAGGATGAAGACAAGGATG
  AAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCTTCAAG
  GATGGGTGATAGTTCACAGGGAGACAACAATGTACAAAAATTAGGTCCTGATGATGTGACTGTGGATATT
  GATGCTATTAGAAGGGTCTACAGCAGTTTGCTCGCTAATGAAAAATTAGAAACTGCCTTCCTGAATGCAC
  TTGTATATCTGTCACCTAACGTGGAATGTGATTTGACATATCATAATGTGTATACTCGAGATCCTAATTA
  TCTCAATTTGTTCATTATTGTAATGGAGAATAGTAATCTCCACAGTCCTGAATATCTGGAAATGGCGTTG
  CCATTATTTTGCAAAGCTATGTGTAAGCTACCCCTTGAAGCTCAAGGAAAACTGATTAGGCTGTGGTCTA
  AATACAGTGCTGACCAGATTCGGAGAATGATGGAAACATTTCAGCAACTTATTACCTACAAAGTCATAAG
  CAATGAATTTAATAGCCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCAAAGTGTTTGAAA
  ATGGTTTACTATGCAAATGTAGTGGGAGGGGATGTGGACACAAATCATAATGAGGAAGATGATGAAGAAC
  CCATACCTGAGTCCAGCGAATTAACACTTCAGGAGCTTCTGGGAGATGAAAGAAGAAATAAGAAAGGTCC
  TCGAGTGGATCCACTAGAAACCGAACTTGGCGTTAAAACTCTAGACTGTCGAAAACCACTTATCTCCTTT
  GAAGAATTCATTAATGAACCACTGAATGATGTTCTAGAAATGGACAAAGATTATACCTTTTTCAAAGTTG
  AAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATCTGGGATT
  ATATTATGACAATAGAATTCGCATGTACAGTGAAAGAAGAATCACTGTTCTTTACAGCCTAGTTCAAGGA
  CAGCAGTTGAATCCGTATTTGAGACTCAAAGTCAGACGTGACCATATTATAGATGATGCACTGGTCCGGC
  TAGAGATGATTGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACA
  AGGAGTAGATGAGGGAGGCGTTTCCAAAGAGTTTTTTCAGTTGGTTGTGGAGGAAATTTTTAATCCAGAT
  ATTGGTATGTTCACATATGATGAAGCTACGAAATTATTTTGGTTTAATCCATCTTCTTTTGAAACTGAGG
  GTCAGTTTACTCTGATTGGCATAGTCCTGGGTCTGGCTATTTACAATAATTGTATACTGGATGTCCATTT
  TCCCATGGTTGTATACAGGAAGCTAATGGGGAAAAAAGGAACCTTTCGTGACTTGGGAGACTCTCACCCA
  GTTTTATATCAGAGTTTAAAGGATTTATTGGAATATGAAGGGAGTGTGGAAGATGATATGATGATCACTT
  TCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAAGAAAATGGTGATAAAAT
  TCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTCTATTCAGACTACATTCTCAATAAATCTGTA
  GAAAAACAATTCAAGGCATTTCGCAGAGGTTTTCATATGGTGACTAATGAATCGCCCTTAAAATACTTAT
  TCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAGGCACTAGAAGAAAC
  TACAGAGTATGACGGTGGCTATACGAGGGAATCTGTTGTGATTAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223606 representing NM_173010
  Red=Cloning site Green=Tags(s)
  
  MKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGAS
  SAYLENSKGASNNSEIKMNKKEGKDFKDVIYLTEEKVYEIYEFCRESEDYSPLIRVIGRIFSSAEALVLS
  FRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDI
  DAIRRVYSSLLANEKLETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENSNLHSPEYLEMAL
  PLFCKAMCKLPLEAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLK
  MVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGDERRNKKGPRVDPLETELGVKTLDCRKPLISF
  EEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQG
  QQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPD
  IGMFTYDEATKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHP
  VLYQSLKDLLEYEGSVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSV
  EKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173010
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173010.3, NP_766598.1
• RefSeq Size: 3888 bp
• RefSeq ORF: 2289 bp
• Locus ID: 22215
• UniProt ID: O08759
• Cytogenetics: 7 33.95 cM
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B (PubMed:20211139, PubMed:24728990). Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo (By similarity). Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990). Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC (PubMed:20211139). Synergizes with WBP2 in enhancing PGR activity (By similarity).[UniProtKB/Swiss-Prot Function]