Etv6 (NM_007961) Mouse Tagged ORF Clone

CAT#: MG223566

Etv6 (tGFP-tagged) - Mouse ets variant gene 6 (TEL oncogene) (Etv6), (10ug)

Product Images

Circular map for MG223566

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Etv6
• Synonyms: AW123102; AW557856; Te; Tel
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223566 representing NM_007961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGACTCCTGCTCAGTCTAGCATTAAGCAGGAACGAATTTCATACACGCCCCCAGAGAGTCCAG
  TGGCAAGCCACCGTTCCTCGACTCCGCTTCATGTTCACACAGTGCCTCGAGCGCTCAGGATGGAGGAAGA
  CTCGATCCACCTGCCAACACACCTGCGTTTGCAGCCGATTTACTGGAGCAGAGATGACGTAGCCCAGTGG
  CTCAAGTGGGCAGAAAATGAGTTTTCTTTAAGGCCCATTGAGAGCAACAAGTTCGAAATGAATGGCAAGG
  CCCTCCTGCTGCTGACCAAAGAGGATTTCCGCTACCGATCTCCTCATTCAGGCGACGTGCTCTATGAACT
  CCTTCAGCATATCCTGAAGCAGAGGAAATCTCGAATGCTCTTCTCACCATTCTTCCCCCCTGGGGACTCT
  ATCCACACCAAGCCAGAGGTCCTCCTGCATCAGAACCATGACGAAGATAACTGTGTCCAGAGGACACCCA
  GGACGCCCGCGGAGAGCGTGCACCACAACCCTCCCACCATCGAACTCTTACATCGCCCTAGGTCACCCAT
  CACCACAAACCACAGGCCTTCTCCTGACCCCGAACAGCAGCGGCCCCAGCGGTCCCCCCTAGACAACATG
  AGCCGCCGCCTCTCGCCAGTGGAGAAAGCCCAGGGGCCCAGGCTACAGCAGGAGAACAACCACCAGGAAA
  CGTACCCCCTGTCAGTGTCTCCTGTCGAGAATAATCACTGCCTGCCCTCAAGCCCCTGGCAGGAGAGCAC
  TCGAGTGATCCAGCTGATGCCCAGCCCCATCATGCACCCTTTGATCCTGAACCCCCGGCACTCGCACTCG
  GTGGACTTCAAACAGTCCCGGCACTCCGAGGATGGGATGAATCGGGAAGGGAAGCCCATCAACCTGTCTC
  ATCGGGAGGACCTGGCTTACTTGAACCACATCATGGTCTCTATGTCCCCACCGGAAGAGCACGCCATGCC
  CATTGGGAGAATAGCAGACTGTAGACTGCTTTGGGATTATGTCTATCAGTTGCTGTCTGACAGCCGGTAC
  GAAAACTTCATCCGATGGGAGGACAAAGAATCCAAAATATTCCGGATAGTGGATCCCAACGGACTGGCTC
  GACTCTGGGGAAACCATAAGAACAGAACAAACATGACCTATGAGAAAATGTCCAGAGCCCTGCGCCACTA
  CTACAAACTAAACATTATCAGGAAGGAGCCCGGACAAAGGCTTTTGTTCAGGTTCATGAAAACCCCAGAT
  GAGATCATGAGTGGCCGGACAGACCGTCTAGAACACCTCGAGTCTCAAGTGCTGGATGAACAAACGTACC
  AAGAGGATGAACCTACCATAGCCTCACCGGTGGGCTGGCCAAGAGGAAACCTGCCCACGGGGACCGCAGG
  AGGCGTGATGGAAGCAGGCGAGCTAGGGGTGGCTGTAAAGGAAGAGACCCGGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223566 representing NM_007961
  Red=Cloning site Green=Tags(s)
  
  MSETPAQSSIKQERISYTPPESPVASHRSSTPLHVHTVPRALRMEEDSIHLPTHLRLQPIYWSRDDVAQW
  LKWAENEFSLRPIESNKFEMNGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKSRMLFSPFFPPGDS
  IHTKPEVLLHQNHDEDNCVQRTPRTPAESVHHNPPTIELLHRPRSPITTNHRPSPDPEQQRPQRSPLDNM
  SRRLSPVEKAQGPRLQQENNHQETYPLSVSPVENNHCLPSSPWQESTRVIQLMPSPIMHPLILNPRHSHS
  VDFKQSRHSEDGMNREGKPINLSHREDLAYLNHIMVSMSPPEEHAMPIGRIADCRLLWDYVYQLLSDSRY
  ENFIRWEDKESKIFRIVDPNGLARLWGNHKNRTNMTYEKMSRALRHYYKLNIIRKEPGQRLLFRFMKTPD
  EIMSGRTDRLEHLESQVLDEQTYQEDEPTIASPVGWPRGNLPTGTAGGVMEAGELGVAVKEETRE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007961
• ORF Size: 1455 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007961.4, NP_031987.3
• RefSeq Size: 5541 bp
• RefSeq ORF: 1458 bp
• Locus ID: 14011
• UniProt ID: P97360
• Cytogenetics: 6 64.58 cM
• Gene Summary: This gene encodes a transcriptional repressor belonging to the ETS family of proteins. Knockout of this gene in mice results in embryonic lethality due to defective angiogenesis. In humans, this gene is often involved in chromosome rearrangements associated with specific cancers. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]