Cenpc1 (NM_007683) Mouse Tagged ORF Clone

CAT#: MG223561

Cenpc1 (tGFP-tagged) - Mouse centromere protein C1 (Cenpc1), (10ug)

Product Images

Circular map for MG223561

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cenpc1
• Synonyms: CENP-C; Cenpc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223561 representing NM_007683
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGTTCCATCTGGATCATCTAAAGAACTACCACAGAAGATATTGCCGGTCTTCCAGAGCACCCA
  ACATTCATACAAAAAAAGGCCAGAACATGCTGGAAATCTTACAAGATTGTTTTGAAGATCAGAGTAAGGC
  TTCATTTCTTGATGATTTTACAGAGTCATTGACTTCTTCAACACAAAAAAAGAAAGCCAATTATAGTCAA
  TCATCAAGCAAAAAGTGCCCAGAGTCACATTCCAAACCAGTTCCAGTGTCTTCAAGGACAGGAGAAGCTT
  CTCTGCAAGCGTCTGCAGAGCCCAGTGAAGCCGCTGGTGGATCAGTTCAGGCTAATGAAGTTCACCATGG
  TGCGTCTGATGAATTGGACTTATGTGTTGGCTCCCCTGTGGTTCTCCTGGATGCAAACGTCAACACGTTA
  CAGAAAGCCGCTTCACCTGCCGGCCAAAAGAGAGTGGCTTCTGTCTCAAGGAGTCCAGTAGATAGGCAGG
  CTTCAAATAAAAACATTTCTTTTAAAACCAGGAAAAGGTTAAACTTTGAAGATAAAGTTACTTTGAGCAC
  AGCAGAAACAGAGAACAGTGTGTTACAAGTAGAGGATAACTTATCCAAGGGACAAGAAGGGACATCATCA
  GAAATCACTCAGAAAAGAGACGATCTGAGTTCTGATGTCCAATCACGTTCTAAGAAGAATTTTTCGGAGT
  TGTTTTTAGAGACTGTCAAGAGGAAAAGTAAATCCAGTTCTGTGGTCAGGCACACAGCAGCAGTTCCATT
  TTCTCCGCCTCCTCCAAGTGACATGAAATTATTAGAAGATGAATTTATTATTGATCGGTCAGATAGAAGT
  TTTTCAAGTCGACTTTGGGTGATGATACCAAGCAAAGACAGGCATCTGAGCGCCCACAAGCCATCTCCTG
  AAAACACTGCACTGCTTCAAGGTAAGAAGTCAAGAGAGAAATCTCATAGTTTATCTGCAATGACGTTTGC
  AAGAAACACACAGTCTGATAAGGCTCACCCAATAGAGGAAGCTCAGCTCTCTGTTGAAGAAAACCCGGCA
  ACTACTTGTACAGATGAGTTGGAAAATGATTGTCGATCTCCTGAAAATAAAATGCAATCTGAGACTGCCA
  AAACCCCACCCGCATGGGAAAGGACTACGAAACAGAGCCAGAGGAGAGTGTCTAAGCCTAAAGCAGCTGA
  AGAGCTCAGGAAGGGACAGAGTAGCTGGGAAAATAGCAATGTGTCAAACACTGGCCAAGACAAGCTACAG
  ATCAACTCGAAAAGAAATATGAAGGACTGTGAAGAGGTGAGGAATGAGCCTAATCCCAAGAAACAGAAGC
  CAGCTCTTGAAAATAAGAAAAAAACCAATAGCACCCAAACCAATAAGGAAAAATCTGGAAAGAAATTTTT
  TTCTGGTGGGTCCAAGAACAAATTTGTTCCCAAAAAAGTAACTTTGACTTCCAGGAGAAGTTGTAGAATT
  TCTCAGCGTCCATCTGAGTGGTGGAGGGTAAAATCAGATGAAAGTTCTGTTGATAGAAATCCTTCAAAAG
  AAAATAACTCACCAGTGGTATATCCCAATAAAAAGAAGCAGACTAAGAGAAATCATGTATCTAAGAGGGC
  TGGGAAGAAACCTGGTTCATCAAAAAGACAGAAGACAGAGATGAGCCCAAGAGTACAGAAGTCTTTAAAT
  GTAAAGGATTCTGGAGGAACTGTTAGTGGCCATGATGACACTTCTCGTTCCCAGAGGAAGCCATTGAAAA
  TCATTGAGGCAGACCCAACTCAGAAGAGCCTTGCTATTTCTCGACCTAAAAGAGGCTGTAAGTATCGAAA
  CAATGTTATGACATCACCGAATGTTCATTTAAAGTCTCATACTGAGGAATATACAAGCAAGACACAAATG
  GAGTCTGCTTCCAATTCAGAAATGTCTAAACGTTCAGTGTGGGAAGAAAGTGGACCTTCCAGGTTCAAGA
  ATTATGAAATGCCAGGGAGCAGTAATTCTGAGATGGGTGATGAACAGGACCAGAAGAGTTTGCATTTTAC
  AACAAGAAGTTTTAATATGGTACCAGATAAAAAATTACATCACAAATTAGTACTGCCCTCCAACTCACCA
  AATGTTCGTCGATCTAATAGAATACGTTTGAAACCTCTGGAGTATTGGCGAGGGGAACGGGTAGATTATC
  AAGAAAGTTCGTCGGGACAACTTGTGCTTGAAATAATATCCCCATCTTCAGTACCCACGAAAATAAAGGC
  ACAAAGAAACCTCGGCAAAGTCAACAAAAAAGTTACCAAGAAGCCGACCCATCTCAATAGCCATGAAAAG
  GCTAAGATGGAGCTGCCTCTGGATATGCGTCTGGGAGATCCCTTTCAGGCAACATTGGCGAAGGACCCAG
  AAACCGCAGAGCTTGTCCCCATGGATCTTATAAGACCACGAGATACATACCGCTTTTTTGTTGAGCAACA
  TGGTTTGAAAGTTTTTAAAACATTGGATACAATATATTTTTCTACTGGAAAATTGGTTTTAGGACCTTAT
  GAAGAGAAAGGAAAACAGCATGTTGGCCAAGATATATTGGTTTTTTATGTTAACTTTGGTGACCTTTTAT
  GTACATTACATGAAACACCTTATAAGTTAACTACTGGGGATTCATTCTATGTTCCTTCAGGTAATCATTA
  CAACATTAAAAACCTCTTGAATGTGGAAAGTAGTCTTCTTTTTACTCAAATAAAAAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223561 representing NM_007683
  Red=Cloning site Green=Tags(s)
  
  MASFHLDHLKNYHRRYCRSSRAPNIHTKKGQNMLEILQDCFEDQSKASFLDDFTESLTSSTQKKKANYSQ
  SSSKKCPESHSKPVPVSSRTGEASLQASAEPSEAAGGSVQANEVHHGASDELDLCVGSPVVLLDANVNTL
  QKAASPAGQKRVASVSRSPVDRQASNKNISFKTRKRLNFEDKVTLSTAETENSVLQVEDNLSKGQEGTSS
  EITQKRDDLSSDVQSRSKKNFSELFLETVKRKSKSSSVVRHTAAVPFSPPPPSDMKLLEDEFIIDRSDRS
  FSSRLWVMIPSKDRHLSAHKPSPENTALLQGKKSREKSHSLSAMTFARNTQSDKAHPIEEAQLSVEENPA
  TTCTDELENDCRSPENKMQSETAKTPPAWERTTKQSQRRVSKPKAAEELRKGQSSWENSNVSNTGQDKLQ
  INSKRNMKDCEEVRNEPNPKKQKPALENKKKTNSTQTNKEKSGKKFFSGGSKNKFVPKKVTLTSRRSCRI
  SQRPSEWWRVKSDESSVDRNPSKENNSPVVYPNKKKQTKRNHVSKRAGKKPGSSKRQKTEMSPRVQKSLN
  VKDSGGTVSGHDDTSRSQRKPLKIIEADPTQKSLAISRPKRGCKYRNNVMTSPNVHLKSHTEEYTSKTQM
  ESASNSEMSKRSVWEESGPSRFKNYEMPGSSNSEMGDEQDQKSLHFTTRSFNMVPDKKLHHKLVLPSNSP
  NVRRSNRIRLKPLEYWRGERVDYQESSSGQLVLEIISPSSVPTKIKAQRNLGKVNKKVTKKPTHLNSHEK
  AKMELPLDMRLGDPFQATLAKDPETAELVPMDLIRPRDTYRFFVEQHGLKVFKTLDTIYFSTGKLVLGPY
  EEKGKQHVGQDILVFYVNFGDLLCTLHETPYKLTTGDSFYVPSGNHYNIKNLLNVESSLLFTQIKR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007683
• ORF Size: 2718 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007683.4
• RefSeq Size: 3192 bp
• RefSeq ORF: 2721 bp
• Locus ID: 12617
• UniProt ID: P49452
• Cytogenetics: 5 E1
• Gene Summary: This gene encodes a centromeric protein component of a nucleosome-associated complex that plays a central role in kinetochore protein assembly, mitotic progression and chromosome segregation. The human ortholog encodes a protein with DNA-binding activity, that associates constitutively to kinetochores throughout the cell cycle, as part of a prekinetochore complex, together with centromeric protein-A and centromeric protein-B. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]