Ano5 (NM_177694) Mouse Tagged ORF Clone

CAT#: MG223543

Ano5 (tGFP-tagged) - Mouse anoctamin 5 (Ano5), (10ug)

Product Images

Circular map for MG223543

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ano5
• Synonyms: 9330162L24; Gdd1; Tmem16e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223543 representing NM_177694
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGCAGGAAGGCTTAACAGCCAAAGAAATAGACTATGCTTTCCAACAGAATGAGAACCTGGGCT
  CCAAAGAGACCAGCTTTCTCATCCCTGAAGACTTACAGTCACCGCCTGAGAAGCGATTCAATTTGTTCCT
  GAGGAGGCGTCTTATGTTCCAGAGAAGTGAGCACAGCAAAGACTCGGTCTTCTTCAGAGATGGAATCCGG
  CAGATCGACTTTGTCCTGTCCTACGTGGAGGATTTGAAGAAAGATGGAGAGCTGAAGGCAGAAAGAAGAC
  GAGAGTTTGAACAAAATCTCAGAAAAACAGGTCTTGACTTGGAAACAGAAGACAAATTGAACTCTGAAGA
  TGGGAAAACCTATTTTGTGAAGATTCACGCCCCATGGGAAGTCCTGGTCACTTACGCTGAAGTGCTGGGA
  ATCAAGATGCCTATTAAGTTGAGTGACATCCCACGGCCAAAGTACCCACCCCTGTCTTACATGCTTGGGG
  CGGTGAAGCTTCCATCCTCTGTGAAGTATCCTACTCCTGAGTATTTCACTGCCCAGTTCAGCAGACACCG
  TCAAGAACTCTTCCTCATTGAAGATGAGGCCACTTTCTTTCCATCCTCAACAAGGAACAGAATTGTTTAC
  TATATTCTGTCACGATGCCCTTTTGGAGTGGAAGAGGGGAAGAAAAAGATTGGGATCGAGAGACTGCTGA
  ACTCGAACACTTATTTATCTGCCTACCCGCTCCATGATGGCCAGTATTGGAAACCATCAAAAACTACTCG
  CCCCAATGAAAGGTACAACCTTTGCAAGAACTGGGCTCGATTTTCCTACTTTTACAAGGAGCAACCTTTT
  CATTTGATTCGGAATTATTTTGGAGAAAAAATCGGTATCTATTTTGTATTTCTCGGGTATTACACAGAAA
  TGCTGTTGTTTGCGGCTCTGGTCGGCTTAGCCTGCTTCATCTATGGCTTGTTATCAATGGAAAATAACCG
  AACTAGCACTGAAATCTGTGACCCTGACATCGGAGGTCAGATGATCATGTGCCCACTTTGCGATGAAGTG
  TGTGATTACTGGAGACTGAACACCACGTGTTTGCATTCAAAGTTCTCCCATCTGTTTGATAACGAGTCGA
  CTGTGTTCTTTGCGCTCTTCATGGGGATCTGGGTCACATTGTTTCTGGAGTTTTGGAAGCAGCGACAAGC
  CAGGCTTGAGTACGAATGGGATCTGGTGGACTTTGAAGAAGAACAGCAGCAGCTCCAGCTTCGGCCTGAA
  TTTGAAGCTATGTGTAAACACAAGAAGATGAATCCTGTGACAAAGGAAATGGAGCCACACATGCCTCTAT
  GCCATCGCATCCCATGGTACTTTGTGTCAGGGACCACAGTGACCTTTGGGATGGCTCTCCTCCTCAGCAG
  CATGGTGTCCATCCTCATATACCGCCTGTCAGTCTTTGCCACTTTTGCCAGCTTCATGGAAAGCGAAGCC
  ACCCTGCAGAGCGTGAAGAGCTTCTTTACTCCACAGCTGGCTACGGCTCTCTCTGGATCATGCCTGAACT
  GCATTGTCATTTTGATACTGAATTTTTTTTATGAGAAGATATCTGCCTGGATTACGAAAATGGAAATACC
  ACGAACTCACCAGGAGTATGAGAGCAGCCTCACTCTGAAGATGTTTCTCTTCCAATTTGTGAACTATTAC
  TCATCTTGCTTCTATGTGGCCTTCTTTAAAGGGAAGTTCGTGGGATATCCTGGGAGTTACACATATATGT
  TCAACATATGGAGAAGCGAAGAGTGTGGTCCTGCAGGCTGTCTGATAGAGCTGACCACCCAGCTAACCAT
  CATTATGATTGGAAAACAGATTTTTGGAAACATCCATGAAGCCTTTCAACCCTTGATTTTTAATTGGTGG
  AGACGCCGAAGAGCCCGAACCCACTCTGAGAAACTGTACAGTCGTTGGGAGCAAGATCATGACCTTCAGG
  TTTATGGACATCGTGGGCTGTTTTATGAGTACTTGGAAACAGTTATCCAGTTTGGGTTTGCCACGCTGTT
  TGTGGCCTCTTTTCCTCTGGCACCCCTGTTTGCTCTCATGAACAACATCATGGGAATCCGAGTGGATGCC
  TGGAAGCTGACAACTCAGTACAGGAGACCTGTCGCAGCGAAAGCCCACTCCATAGGTGTCTGGCAAGACA
  TTCTTTTTGGAATGGCCATCGTTTCTGTTGCAACTAATGCCTTCATTGTCTCATTTACATCTGACATCAT
  CCCCCGGTTAGTTTACTTCTATGCCTACTCAACGAATTCCACAGAGCCCTTGAGTGGATACGTCAATAAC
  AGCCTGTCAGTGTTCCTGATAGCTGACTTTCCCAACCACACAGTGCCCATGGAGAAAAAAGACTTCGTCA
  CCTGCAGGTACAGAGATTATAGGTACCCTCCTGATCATGAGGATAAGTATAGCCATAACATGCAGTTCTG
  GCATGTTCTTGCTGCCAAGATGACCTTCATCATAGTGATGGAACACGTCGTGTTTCTGTTTAAATTTTTA
  TTAGCCTGGCTGATACCAGATGTTCCTAAAGATGTTGTGGAGAAAATCAAACGAGAAAAGTTAATGACTA
  TCAAAATTATTCATGACTTTGAGCTCAACAAGTTGAAAGAAAACTTGGATGTTGAATATGGTAACATCAT
  GAAGAATGTTTTAGTAGATGAAGACAATTCCCTGAAGGCTAAAACTACAGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223543 representing NM_177694
  Red=Cloning site Green=Tags(s)
  
  MVEQEGLTAKEIDYAFQQNENLGSKETSFLIPEDLQSPPEKRFNLFLRRRLMFQRSEHSKDSVFFRDGIR
  QIDFVLSYVEDLKKDGELKAERRREFEQNLRKTGLDLETEDKLNSEDGKTYFVKIHAPWEVLVTYAEVLG
  IKMPIKLSDIPRPKYPPLSYMLGAVKLPSSVKYPTPEYFTAQFSRHRQELFLIEDEATFFPSSTRNRIVY
  YILSRCPFGVEEGKKKIGIERLLNSNTYLSAYPLHDGQYWKPSKTTRPNERYNLCKNWARFSYFYKEQPF
  HLIRNYFGEKIGIYFVFLGYYTEMLLFAALVGLACFIYGLLSMENNRTSTEICDPDIGGQMIMCPLCDEV
  CDYWRLNTTCLHSKFSHLFDNESTVFFALFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPE
  FEAMCKHKKMNPVTKEMEPHMPLCHRIPWYFVSGTTVTFGMALLLSSMVSILIYRLSVFATFASFMESEA
  TLQSVKSFFTPQLATALSGSCLNCIVILILNFFYEKISAWITKMEIPRTHQEYESSLTLKMFLFQFVNYY
  SSCFYVAFFKGKFVGYPGSYTYMFNIWRSEECGPAGCLIELTTQLTIIMIGKQIFGNIHEAFQPLIFNWW
  RRRRARTHSEKLYSRWEQDHDLQVYGHRGLFYEYLETVIQFGFATLFVASFPLAPLFALMNNIMGIRVDA
  WKLTTQYRRPVAAKAHSIGVWQDILFGMAIVSVATNAFIVSFTSDIIPRLVYFYAYSTNSTEPLSGYVNN
  SLSVFLIADFPNHTVPMEKKDFVTCRYRDYRYPPDHEDKYSHNMQFWHVLAAKMTFIIVMEHVVFLFKFL
  LAWLIPDVPKDVVEKIKREKLMTIKIIHDFELNKLKENLDVEYGNIMKNVLVDEDNSLKAKTTV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177694
• ORF Size: 2712 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177694.6, NP_808362.2
• RefSeq Size: 7785 bp
• RefSeq ORF: 2715 bp
• Locus ID: 233246
• UniProt ID: Q75UR0
• Cytogenetics: 7 B4
• Gene Summary: This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. Elevated levels of this protein were found in dystrophic mice. In humans, mutations of this gene are associated with with musculoskeletal disorders such as myopathies, muscular dystrophy and gnathodiaphyseal dysplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]